Wax is an acellular structural substance attached to the surface of plant tissues. It forms a protective barrier on the epidermis of plants and plays an important role in resisting abiotic and biotic stresses. In this paper, nonheading Chinese cabbage varieties with and without wax powder were observed using scanning electron microscopy, and the surface of waxy plants was covered with a layer of densely arranged waxy crystals, thus differentiating them from the surface of waxless plants. A genetic analysis showed that wax powder formation in nonheading Chinese cabbage was controlled by a pair of dominant genes. A preliminary bulked segregant analysis sequencing (BSA-seq) assay showed that one gene was located at the end of chromosome A09. Within this interval, we identified BraA09000626, encoding an AP2 transcription factor homologous to Arabidopsis AtSHINE3, and we named it BrSHINE3. By comparing the CDS of the gene in the two parental plants, a 35 bp deletion in the BrSHINE3 gene of waxless plants resulted in a frameshift mutation. Tissue analysis showed that BrSHINE3 was expressed at significantly higher levels in waxy plant rosette stage petioles and bolting stage stems than in the tissues of waxless plants. We speculate that this deletion in BrSHINE3 bases in the waxless material may inhibit wax synthesis. The overexpression of BrSHINE3 in Arabidopsis induced the accumulation of wax on the stem surface, indicating that BrSHINE3 is a key gene that regulates the formation of wax powder in nonheading Chinese cabbage. The analysis of the subcellular localization showed that BrSHINE3 is mainly located in the nucleus and chloroplast of tobacco leaves, suggesting that the gene may function as a transcription factor. Subsequent transcriptome analysis of the homology of BrSHINE3 downstream genes in nonheading Chinese cabbage showed that these genes were downregulated in waxless materials. These findings provide a basis for a better understanding of the nonheading Chinese cabbage epidermal wax synthesis pathway and provide important information for the molecular-assisted breeding of nonheading Chinese cabbage.

Cytokinins (CKs) are among the hormones that regulate plants’ growth and development, and the CKX and IPT genes, which are CK degradation and biosynthesis genes, respectively, play important roles in fine-tuning plants’ cytokinin levels. However, the current research on the function of IPT and CKX in cucumber’s growth, development, and response to abiotic stress is not specific enough, and their regulatory mechanisms are still unclear. In this study, we focused on the IPT and CKX genes in cucumber, analyzed the physiological and biochemical properties of their encoded proteins, and explored their expression patterns in different tissue parts and under low light, salt stress, and drought stress. Eight CsCKX and eight CsIPT genes were identified from the cucumber genome. We constructed a phylogenetic tree from the amino acid sequences and performed prediction analyses of the cis-acting elements of the CsCKX and CsIPT promoters to determine whether CsCKXs and CsIPTs are responsive to light, abiotic stress, and different hormones. We also performed expression analysis of these genes in different tissues, and we found that CsCKXs and CsIPTs were highly expressed in roots and male flowers. Thus, they are involved in the whole growth and development process of the plant. This paper provides a reference for further research on the biological functions of CsIPT and CsCKX in regulating the growth and development of cucumber and its response to abiotic stress.

Nickel (Ni) is an essential trace element for plant growth and a component of the plant body that has many different functions in plants. Although it has been confirmed that nickel ions (Ni²⁺) havea certain regulatory effect on nitrogen (N) metabolism, there are not enough data to prove whether exogenous Ni²⁺ can increase the carbon (C) and N metabolism in the roots of tomato seedlingsunder low-nitrogen (LN) conditions. Therefore, through the present experiment, we revealed the key mechanism of Ni²⁺-mediated tomato root tolerance to LN levels. Tomato plants were cultured at two different N levels (7.66 and 0.383 mmol L⁻¹) and two different Ni²⁺ levels (0 and 0.1 mg L⁻¹ NiSO₄ 6H₂O) under hydroponic conditions. After nine days, we collected roots for physiological, biochemical, and transcriptome sequencing analyses and found that the activities of N assimilation-related enzymes decreased at LN levels. In contrast, Ni²⁺ significantly increased the activities of N assimilation-related enzymes and increased the contents of nitrate (NO₃⁻), ammonium (NH₄⁺), and total amino acids. Through root transcriptomic analysis, 3738 differentially expressed genes (DEGs) were identified. DEGs related to C and N metabolism were downregulated after LN application. However, after Ni²⁺ treatment, PK, PDHB, GAPDH, NR, NiR, GS, GOGAT, and other DEGs related to C and N metabolism were significantly upregulated. In conclusion, our results suggest that Ni²⁺ can regulate the C and N metabolism pathways in tomato roots to alleviate the impact of LN levels.

To improve the borehole sealing effect, especially that of coal seam with low permeability and micro fissures, this paper takes the expansion rate of the sealing material as the response value and establishes the quadratic model embracing the expansion rate and various experimental factors by designing orthogonal experiments. The response surface is used to further determine the significance order of each key factor according to the expansion rate and adjust the admixture content to obtain the optimal ratio of the sealing material. For the research investigating a sealing material, the optimal ratio of the sealing material is obtained: the content of water reducing agent of 0.5%, the content of retarder of 0.04%, water‐cement ratio of 0.8, and the content of expansion agent of 10%. At this time, the expansion rate reaches 3.136%. Besides, a scanning electron microscope is used to observe the microscopic morphology of the material. According to the scanning electron microscopy analysis of new borehole sealing materials, the surface of the new borehole sealing material shows no holes and possesses compactness; and a large amount of ettringite is formed on the surface of the hydration product of hardened cement slurry. The ettringite improves the expansibility of the material. The new sealing material provides a new idea for gas sealing, which is of great significance to improve the efficiency of borehole extraction, improve the utilization rate of resources, and prevent gas accidents.

NCED proteins play a critical role in drought, salt, and cold stress responses through ABA biosynthesis in plants. However, little information is currently available regarding the NCED gene family in Brassica species. Twenty-six putative BjNCED genes were identified in the genome of Brassica juncea, and found to be distributed on 15 chromosomes. Phylogenetic analysis suggested that these members could be classified into six subfamilies. The putative cis-elements were identified in the promoter regions of these BjNCED genes, and thought to be related to phytohormones, light, and abiotic stress responses. qRT-PCR analysis of five genes (BjNCED1, BjNCED4, BjNCED7, BjNCED16 and BjNCED18) revealed that, under salt stress, BjNCED16 and BjNCED18 showed transient induction, whereas exogenous ABA produced gene-specific and time-dependent expression patterns, including pronounced induction of BjNCED16 at 24 h. Low-temperature treatment strongly induced four of the five examined genes at 6 h. Analysis of the BjNCED genes in this study provides a valuable foundation for future investigations into the functional roles of the BjNCED family in response to growth, development and stress.

The ratio of red to far-red (FR) light (the R/FR ratio) is a core signal regulating shade-induced internode elongation in plants, but the underlying mechanism in cucumber remains not fully elucidated. Therefore, in the present study, cucumber (Cucumis sativus L. ‘Zhongnong No. 26′) was used as the material, and through a combination of physiological, transcriptomic, and proteomic analyses, we investigated the mechanism underlying internode elongation via R/FR ratio in cucumber plants. Low R/FR ratio significantly promoted cucumber seedling internode elongation by enhancing the longitudinal growth of internode cells. Hormonal profiling decreased the contents of indole-3-acetic acid (IAA), salicylic acid (SA), cytokinin (CTK; tZR, T-zeatin, and iPR), and ethylene (Eth), whereas jasmonic acid (JA) and gibberellin (GA) contents were elevated in cucumber seedling internodes. Bioinformatics analysis revealed that low R/FR ratio upregulated GA biosynthesis-related genes (KAO, GA20ox), and the expression of genes/proteins encoding cell wall remodeling/biosynthesis enzymes (EXP, XTH and CesA), and aquaporin proteins (AQPs). Exogenous GA4/7 mimicked the promotive effect of low R/FR on internode elongation under white light, while the GA biosynthesis inhibitor paclobutrazol (PAC) reversed this response, confirming a central role for GA in this process. These results reveal the crucial role of cell wall biosynthesis and hormone metabolism-related genes/proteins in low R/FR ratio-induced cucumber internode elongation. Such work may further elucidate the evolutionary and agronomic trade-offs associated with shade avoidance and provide a basis for optimizing canopy architecture and light management in cucumber cultivation.