Rosmarinus officinalis belongs to the Lamiaceae family, and its constituents show antioxidant, anti-inflammatory, antidepressant, antinociceptive, and antibacterial properties. The aim of this study was to develop a topical formulation with R. officinalis extract that had antimicrobial and antioxidant activity. Maceration, infusion, Soxhlet, and ultrasound were used to produce rosemary extracts, which were submitted to antioxidant, compound quantification, cell viability, and antimicrobial assays. Infusion and Soxhlet showed better results in the DPPH assay. During compound quantification, infusion showed promising metabolite extraction in phenolic compounds and tannins, although maceration was able to extract more flavonoids. The infusion and ultrasound extracts affected more strains of skin bacteria in the disk diffusion assays. In the minimum inhibitory concentration assay, the infusion extract showed results against S. aureus, S. oralis, and P. aeruginosa, while ultrasound showed effects against those three bacteria and E. coli. The infusion extract was chosen to be incorporated into a green emulsion. The infusion extract promoted lower spreadability and appropriated the texture, and the blank formulation showed high levels of acceptance among the volunteers. According to the results, the rosemary extract showed promising antioxidant and antimicrobial activity, and the developed formulations containing this extract were stable for over 90 days and had acceptable characteristics, suggesting its potential use as a phytocosmetic. This paper reports the first attempt to produce an oil-in-water emulsion using only natural excipients and rosemary extract, which is a promising novelty, as similar products cannot be found on the market or in the scientific literature.

Thirteen Haemophylus influenzae invasive strains isolated from patients at Clinical Hospital of State University of Campinas, from May 2013 through August 2019, was submitted to Illumina genome sequencing HiSeq platform. Further in silico analysis of serogroup and Multi Locus Sequence Typing (MLST) from whole DNA sequencing had demonstrated the actual clonal distribution in the Campinas Metropolitan region. Thus, results showed the existence of a new ST Haemophilus influenzae found in the Brazilian territory and an increase of strains belonging to serogroup a (three strains also belonging to ST23). In conclusion, we observed an increase of non-typable H. influenzae (NTHi) and a strain involved in invasive diseases in the Campinas – São Paulo region after frequent detection of those serotypes and genotypes in other Brazilian regions.

Several vaccine prototypes were made using variations in concentration, volume, time and speed of agitation. From these prototypes, some characterization assays were carried out, such as the analysis of particle size and zeta potential, where it was possible to determine the size of the nanoparticles, the polydispersity index, with the most promising samples having a size of 252.1nm ± 71.2 nm. The polydispersity index was 0.572 ±0.02, the standard deviation being a little high due to the presence of free viral particles and some associated vesicles. It is noteworthy that the isolated vesicle has a size of 140 to 160nm and the polydispersity index of 0.513 demonstrates that we do not have interference in the sample of our vesicle (it is known that the size of the viral particle of Zika virus is between 40 and 60nm). Cytotoxicity testing was done to analyze whether they have cytotoxic profiles and also demonstrate that the prototype's neutralization and inactivation method works correctly. The analysis of the protein profile of some of the samples revealed that there are two major Zika virus proteins in the fused proteasome, namely the E protein, which is directly involved in the recognition of host cell receptors, and the C protein, which is related to capsid assembly, in addition to playing an important role in the immune response.

The fifty-two bacteria were isolated from urinary tract infections in University Hospital in Sumaré, São Paulo State, Brazil. These isolates were analyzed about their antibiotic resistance and their bacterial characteristics. Around percentage from these isolates were identified as belonging to Escherichia coli strains, followed by Klebsiella pneumoniae and Pseudomonas aeruginosa. The multiresistance profile (resistance profile major than four 4 antibiotic classes) were viewed in thirteen (13) strains of Escherichia coli, sixteen (16) strains in Klebsiella pneumoniae (being two (2) carbapenem resistant suggesting an existence of KPC strain). The results had demonstrated an indiscriminate antibiotic use concentrated in the increase of quinolones resistance (principally in ciprofloxacin use), and also the rise of KPC super bacteria.