The rice weevil, Sitophilus oryzae (L.), is the most destructive insect pest of stored cereals worldwide. The current study was conducted to determine the lethal, reproductive, and histological effects of gamma irradiation on S. oryzae adults. In addition, the impact on germination, chlorophyll, and proline content in wheat seedlings from treated grains was determined. Wheat grains were infested with rice weevil adults and then irradiated by gamma rays. Gamma radiation was applied at a dosage of 0.10, 0.25, 0.50, and 1.00 kGy. Mortality percentage and LD₅₀ were recorded after 48, 72, 96, and 120 h of treatment. The dosage of 1.00 kGy caused 100% mortality after 96 h of irradiation. The required dosage of gamma radiation to kill 50% (LD₅₀) of adults after 48 h was 1.51 kGy. All tested doses caused complete sterility to 24 h old adults. A histological alteration was noticed at a dosage of 1.00 kGy, which showed cytoplasmic vacuolization, tissues exhibiting signs of putrefaction, and necrosis of cells; furthermore, gamma irradiation affected chlorophyll a and b. The highest amounts were detected in wheat seedlings from grains irradiated at 0.10 kGy. There was a significant increase in plant proline content at the higher doses (0.50 and 1.00 kGy) compared with seedlings from nonirradiated grains. It could be concluded that gamma radiation can be used as an eco-friendly trend to control stored-product pests without any residual effects.

The silverleaf whitefly (Bemisia tabaci) is one of the most harmful insects attacking several economic plant crops worldwide, and it has developed a resistance toward several conventional insecticides. This study was conducted to estimate the impact of potassium phosphite (PK), effective microorganisms (EMs), and salicylic acid (SA) as plant inducers, and imidacloprid (IMI) as a synthetic insecticide on the systemic acquired resistance of sweet pepper (Capsicum annuum var. annuum) crop, whitefly population, and crop yield under greenhouse conditions. The treatment plots were sprayed with IMI, PK, EMs, SA, and water (control) on the 27th day after planting, and dinotefuran was applied when the whitefly-infestation ratio reached 3.00%. The enzymes responsible for the internal defence system, whitefly population, and crop yield were determined. Our results confirmed the idea that the PK, EMs, and SA may induce the synthesis of plant enzymes responsible for the internal defence system. The IMI, PK, EMs, and SA significantly suppressed the whitefly population compared with the control. Moreover, the reduction percentages of the whitefly population were significantly higher when using IMI and PK than EMs and SA. The IMI, PK, EMs, and SA improved the crop yield. It could be concluded that PK, EMs, and SA enhanced the systemic acquired resistance in sweet pepper crop causing high defence against the population of whitefly and might be a potent alternative to conventional insecticides and compatible with an integrated pest management program.

The cotton leafworm, Spodoptera littoralis (Bosid.), is a major pest in African and Asian nations that attacks a wide variety of host plants. This study was conducted to assess the effectiveness of effective microorganisms (EMs) on the biological and physiological features of S. littoralis larvae. Five concentrations (100, 200, 300, 400, and 500 ppm) of EMs were tested. Antifeedant activity, food consumption index, the efficiency of converting digested food, the efficiency of converting ingested food, relative growth rate, and approximate body tissue of the fourth larval instar of S. littoralis were determined. Moreover, carbohydrate enzyme activities (amylase, trehalose, and invertase), total protein, and total lipids of S. littoralis larvae were measured to elucidate the mode of action of the tested agent in the S. littoralis’s larval stage. The EMs at 500 ppm had a substantial impact on antifeedant activity, nutritional indices, egg deposit reduction, and hatchability in S. littoralis during the five days. All concentrations interrupted S. littoralis’s life cycle and developmental phases. Furthermore, all concentrations were quite useful in lengthening the developmental stages of S. littoralis. In addition, Ems affected the biochemical activities of larvae, leading to disturbances in carbohydrate, lipid, and protein levels. From this study, EMs can be used as a bioinsecticide alternative to traditional insecticides against S. littoralis and may be compatible with integrated pest management approaches.

Under laboratory conditions, the sub-lethal effects of four agents [silica bulk, silica nanoparticles (NPs), two synthesis agents (silica bulk-abamectin, silica NPs-abamectin)] were compared to one synthetic acaricide (abamectin) against Tetranychus urticae immature stages. Sublethal concentrations (LC25) of the tested agents against adult females of T. urticae were determined using standard bioassay procedures. The effects of sublethal concentrations of the investigated compounds on egg deposition and egg hatchability were measured over five days at 22 and 28 degrees Celsius, respectively. The effects of the investigated substances on the duration (in hours) of T.urticae developmental phases were evaluated at two different temperatures 22 and 28 oC. The results showed that silica nanoparticles (NPs) coated with abamectin had a significant influence on egg deposited decrease by T. urticae adult females. By the way, silica NPs-abamectin was the most effective therapy for egg deposition and hatchability over five days at 22 and 28 oC respectively, followed by abamectin. Furthermore, silica NPs-abamectin was found to be very beneficial in prolonging the length (in hours) of T.urticae developmental stages at different temperatures. This research reveals that silica bulk and silica nanoparticles (NP) might be employed as replacements for conventional acaricides and are compatible with IPM approaches. They could be useful in the future for pest management.