Coiled planar capillary chromatography columns (0.9 mm I.D. × 60 cm L) were 3D printed in stainless steel (316L), and titanium (Ti–6Al–4V) alloys (external dimensions of ∼5 × 30 × 58 mm), and either slurry packed with various sized reversed-phase octadecylsilica particles, or filled with an in situ prepared methacrylate based monolith. Coiled printed columns were coupled directly with 30 × 30 mm Peltier thermoelectric direct contact heater/cooler modules. Preliminary results show the potential of using such 3D printed columns in future portable chromatographic devices.

This review covers the latest developments and applications of nano-materials in stationary phase development for various modes of high-performance liquid chromatography. Specific attention is placed upon the development of new composite phases, including the synthetic and immobilisation strategies used, to produce either encapsulated nano-particles, or surface attached nano-particles, layers, coatings and other structures. The resultant chromatographic applications, where applicable, are discussed with comment upon enhanced selectivity and/or efficiency of the nano-particle modified phases, where such effects have been identified. In the main this review covers developments over the past five years and is structured according to the nature of the nano-particles themselves, including carbonaceous, metallic, inorganic, and organopolymer based materials.

This review covers the latest developments and applications of porous layer open tubular columns in capillary liquid chromatography. Here, the authors provide a concise background on the fundamentals of porous layer open tubular columns, their fabrication and application. Over the past two decades, growing interest in the areas of proteomics and hyphenated LC-MS techniques have played a large part in the development of porous layer structures within capillary formats due to their high permeability, excellent efficiency and exceptional peak capacity. This review gives a brief overview of open tubular columns in general, however, it focuses predominantly on the applications of covalently bonded porous layer open tubular columns in liquid chromatography. Open tubular columns containing non-bonded or electrostatically attached layers will not be discussed in detail.