Using the two-tooth difference swing-rod movable teeth transmission system satisfying the drive function of large optical instruments as the study object, the influence of each component error on system transmission error is analysed. Each component error is presented by the vector method, and then it is transformed into equivalent error in the direction of the meshing action line based on the equivalent meshing error principle. The instantaneous transmission ratio of the system is obtained by the instantaneous velocity center method, and the system transmission error model is established. Using numerical analysis, the influence of each component error on the system transmission error is obtained. The transmission error test platform is used to test and analyze the transmission error of the two-tooth difference swing-rod movable teeth transmission system. The research results show that the swing-rod length error and the wave generator eccentric error have a great influence on the transmission error of the system among the six types of error factors, so they should be strictly controlled during design, processing and assembly. This study provides a theoretical basis for the rational allocation of machining errors and assembling errors of the two-tooth difference swing-rod movable teeth transmission system.