To ensure the better production and sustainable management of natural resources, a chemometric investigation was conducted to examine the effect of cooperative and harvesting periods on the crop yields and chemical compositions of Salvia rosmarinus Spenn essential oils in the Oriental region of Morocco. The samples were collected from three cooperatives over nine time periods from January 2018 to April 2019. The chemical composition of Salvia rosmarinus Spenn essential oils was analyzed by gas chromatography coupled with mass spectrometry. The data from this study were processed by multivariate analyses, including principal component analysis (PCA) and hierarchical cluster analysis (HCA). The disc diffusion technique and a determination of the minimal inhibitory concentration were performed to study the antibacterial properties of the oils. Statistical analysis showed that the cooperative and harvest period have a significant effect on yields. The highest yield of essential oil was recorded in April 2019 at cooperative C1. The PCA and the HCA results were divided into two groups: Group A for the summer season and group B for the winter season. The samples collected during summer were characterized by a high amount of 1,8-cineole component and a high yield of essential oil, whereas the samples collected during winter were qualified by a high amount of α-pinene component and a low yield of essential oil. The antibacterial activity of Salvia rosmarinus Spenn essential oils showed that Mycobacterium smegmatis ATCC23857 and Bacillus subtilis ATCC 23857 are the most susceptible strains, stopping growth at 1/500 (v/v). The least susceptible strain is Escherichia coli ATCC25922, with an MIC value corresponding to 1/250 (v/v). The findings of this study could have a positive economic impact on the exploitation of rosemary in the Oriental region, especially during the best harvest periods, as they indicate how to obtain the best yields of oils richest in 1,8-cineole and α-pinene chemotypes.

The present study investigates the insecticidal effect of plant extract such as Mentha pulegium and Rosmarinus officinalis essential oils and some of their major compounds; these plants are well known for their many biological activities. The fumigant toxicity was evaluated, using glass jars, against female adults of Culex pipiens that constitute a mosquito vector of important diseases such as the West Nile virus. The adulticidal test showed that both essential oils and monoterpenes presented an insecticidal effect better than the chemical insecticide (Deltamethrin). The highest mortality percentages for the two essential oils have occurred at 312.5 µL/L air (between 56.14 ± 1.7% and 97.71 ± 3.03% after 24 h and 48 h of treatment). Moreover, all tested monoterpenes (carvone, R(+)-pulegone, 1,8-cineole, camphor and α-pinene) have produced high mortalities that varied depending on the time of the treatment and the concentrations used. Lethal concentrations (LC₅₀) obtained for the essential oils and the main compounds have also varied according to the exposure time. M. pulegium and R. officinalis essential oil exhibited the lowest LC₅₀ values after 24 h (72.94 and 222.82 µL/L air, respectively) and after 48 h (25.43 and 55.79 µL/L air, respectively) while the pure molecules revealed the lowest LC₅₀ values after 48 h (between 84.96 and 578.84 µL/L air). This finding proves that the two essential oils and their main compounds have an insecticidal potential, which could help to develop natural toxic fumigants that may be used as an eco-friendly alternative in integrated and sustainable vector management.

The Culex pipiens mosquito (Diptera: Culicidae) is highly suspected to be the vector responsible for the spread of several parasitic and viral diseases. The use of synthetic insecticides is generally the preferred method of controlling these mosquitoes proliferation. However, it has led to resistance problems in target mosquitoes and environmental damage. Hence, diverse plant extracts could be considered as an alternative and potential source as mosquito control agents. In this study, essential oils of Lavandula angustifolia subsp. angustifolia and Lavandula dentata spp. dentata that are growing in Morocco were examined for their insecticidal effects on Culex pipiens larvae. The bioassay was performed according to a methodology inspired by the standard protocol of the World Health Organization. The mortality rate was determined after 24 hours of exposure, and probit regression analysis was used to calculate LC₅₀ and LC₉₀. The chemical analysis revealed that the principal compounds of L. angustifolia subsp. essential oils include linalool, linalyl acetate, geraniol, lavandulyl acetate, camphor, β-caryophyllene, terpinen-4-ol, β-myrcene, and 1,8-cineole, while the essential oil of L. dentata spp. was mainly composed of 1,8-cineole, camphor, α-pinene, trans-pinocarveol, linalool, and borneol. These volatile compounds have shown a toxic effect against Culex pipiens larvae, with lethal concentrations LC₅₀ and LC₉₀ being, respectively, 140µg/ml and 450µg/ml, for the L. angustifolia subsp. essential oil. Meanwhile, they were estimated at 2670µg/ml and 7400µg/ml, respectively, for the L. dentata spp. essential oil. These results suggest using essential oils of two species of Lavandula to control the Culex pipiens mosquito. It could be useful for the study of new natural larvicidal compounds.

Objective: To study the larvicidal activity of essential oils of two aromatic and medicinal\ud plants, Citrus aurantium (C. aurantium) and Citrus sinensis (C. sinensis) (Rutaceae) cultivated\ud in North Eastern Morocco, against the larvae of the malaria vector Anopheles labranchiae (An.\ud labranchiae) (Diptera: Culicidae).\ud Methods: Biological tests were realized according to a methodology inspired from standard\ud World Health Organization protocol. The mortality counts were made after 24 h and LC50 and\ud LC\ud 90 values were calculated.\ud Results: Bioassays revealed that these oils had remarkable larvicidal properties. The minimum\ud levels necessary to achieve 100% mortality of An. labranchiae larvae were evaluated at\ud 160 mg/L for C. aurantium and 640 mg/L for C. sinensis. Essential oil of C. aurantium\ud remained the most efficient (LC50 = 22.64 mg/L, LC90 = 83.77 mg/L), while those of C.\ud sinensis was the least (LC50 = 77.55 mg/L, LC90 = 351.36 mg/L).\ud Conclusions: These results suggest that the essential oils isolated from Citrus plants have the\ud potential to be used as an ideal ecofriendly approach for the control An. labranchiae

Background. Parasitic diseases, in particular leishmaniasis, are still a public health problem in several countries and in Morocco. Methods. The data used are those of epidemiological surveillance collected in the registers of the prefectural epidemiology cell (PEC); however, the socioeconomic data were obtained from the High Commissioner for Planning. The Pearson correlation test was used to determine the correlation between the different variables. Results. In total, 70 cases were recorded by the prefectural epidemiology cell (PEC) during the period from 2009 to 2015. 46% of the cases come from rural areas while 54% of the cases come from urban areas. The Pearson test shows the existence of a significant relationship between the number of cases recorded and the type of environment (r = 0.49, p value = 0.02), and population rate (R = 0.849 and p≤0.001). However, in our case, the poverty rate does not influence CL’s distribution. Conclusion. Our results show that the CL affects the majority of the municipalities with predominance of the urban environment, so the distribution of cases of this pathology is not influenced by the poverty; however, the urbanization and the number of inhabitants have a positive impact on the distribution of this scourge.

Cutaneous leishmaniases (CL) are endemic in Morocco. They are common in the human population in different localities such as Aichoun in Sefrou province, Morocco. This study was carried out in Aichoun locality from April to October 2012 in order to study the spatiotemporal trends of the main Leishmania phlebotomine vectors in this focus. Overall, 1171 sand flies, belonging to four species, were collected by sticky traps. Phlebotomus sergenti was the predominant species (78.4%) followed by Ph. perniciosus (10.5%), Ph. papatasi (7.94%), and Ph. longicuspis (3.16%). Sandflies were active during 6 months (MayOctober). Ph. sergenti, Ph. perniciosus, and Ph. papatasi displayed a bimodal distribution with a first peak in July and a second peak in September, while Ph. longicuspis showed a monophasic trend with a peak in August. The high abundance and the lengthy period of activity of Ph. sergenti and Ph. perniciosus, vectors of L. tropica and L. infantum, respectively, are a cause for concern as they indicate the high potential risk of Leishmania transmission in the studied areas.