An H-shaped photonic crystal fiber (PCF)-based surface plasmon resonance (SPR) sensor is proposed for detecting large refractive index (RI) range which can either be higher or lower than the RI of the fiber material used. The grooves of the H-shaped PCF as the sensing channels are coated with gold film and then brought into direct contact with the analyte, which not only reduces the complexity of the fabrication but also provides reusable capacity compared with other designs. The sensing performance of the proposed sensor is investigated by using the finite element method. Numerical results show that the sensor can work normally in the large analyte RI (n_a) range from 1.33 to 1.49, and reach the maximum sensitivity of 25,900 nm/RIU (RI units) at the n_a range 1.47–1.48. Moreover, the sensor shows good stability in the tolerances of ±10% of the gold-film thickness.

A surface plasmon resonance (SPR) sensor based on a dual-side polished microstructured optical fiber (MOF) with a dual core is proposed for a large analyte refractive index (RI; n_a) detection range. Gold is used as a plasmonic material coated on the polished surface, and analytes can be directly contacted with the gold film. The special structure not only facilitates the fabrication of the sensor, but also can work in the n_a range of 1.42–1.46 when the background material RI is 1.45, which is beyond the reach of other traditional MOF-SPR sensors. The sensing performance of the sensor was investigated by the wavelength and amplitude interrogation methods. The detailed numerical results showed that the proposed sensor can work effectively in the n_a range of 1.35–1.47 and exhibits higher sensitivity in the n_a range of 1.42–1.43.

Retinitis pigmentosa (RP) is a group of retinal diseases that cause the progressive death of retinal photoreceptor cells and eventually blindness. Mutations in the β-domain of the phosphodiesterase 6 (Pde6b) gene are the most identified causes of autosomal recessive RP. Clinically, there is no effective treatment so far that can stop the progression of RP and restore the vision. Here, we report a base editing approach in which adeno-associated virus (AAV)-mediated adenine base editor (ABE) delivering to postmitotic photoreceptors was conducted to correct the Pde6b mutation in a retinal degeneration 10 (rd10) mouse model of RP. Subretinal delivery of AAV8-ABE corrected Pde6b mutation with averaging up to 20.79% efficiency at the DNA level and 54.97% efficiency at the cDNA level without bystanders, restored PDE6B expression, preserved photoreceptors, and rescued visual function. RNA-seq revealed the preservation of genes associated with phototransduction and photoreceptor survival. Our data have demonstrated that base editing is a potential gene therapy that could provide durable protection against RP.