Viral diseases have not previously been described in lettuce (Lactuca sativa) in Puerto Rico. In April 2013, lettuce samples from a hydroponic greenhouse in Guayanilla were submitted to the Plant Disease Clinic at the University of Puerto Rico's Juana Díaz Experimental Station. Lettuce plants were symptomatic for virus and had thrips. Eight samples reacted with Tomato spotted wilt virus (TSWV) DAS-ELISA (Envirologix, Portland, ME) and lateral flow immunoassay (Envirologix). Further sampling at the hydroponic greenhouse, which had 45,000 lettuce plants in different growth stages, revealed leaf symptoms of necrotic ringspots and browning with an incidence of 38%. Losses were high because plants had to be destroyed, resulting in $160,000 of lost earnings to date. Symptoms appeared in the younger core leaves 5 days after transplanting and consisted of small chlorotic spots that developed into necrotic ringspots. The leaves became pale, then brown and wilted. In 15-day-old plants, lesions coalesced and within 1 to 2 days, leaf tissue appeared burned. Soft rot in the crown was observed in 5% of the affected plants. Stunting was also observed when young plants were affected. Due to recent identification of Tomato chlorotic spot virus (TCSV) in Puerto Rico (4) and known cross reaction of TSWV serological reagents with closely related tospoviruses, plants were tested for TCSV, TSWV, and Groundnut ringspot virus (GRSV) by reverse transcription (RT)-PCR as previously described (4). Total RNA was extracted from representative symptomatic leaves of two lettuce plants using RNeasy Plant Mini Kit (Qiagen, Valencia, CA) and tested by RT-PCR with TCSV-specific nucleocapsid (N) or RNA-dependent RNA polymerase (L) gene primers (4) or movement protein (NSm) gene primers (2). Amplicons of the expected sizes were produced with all three TCSV primer sets from both samples, whereas primers specific for the N gene of TSWV (1) or GRSV (3) did not amplify products from either sample. Three TCSV amplicons (N, L, and NSm) from one sample were gel-purified and cloned (pGEM-T, Promega, Madison, WI). Six clones of each amplicon were sequenced in both directions and consensus sequences were deposited in GenBank (KF819827 to 29). All three genes showed greater than 96% nucleotide identity with all TCSV isolates in GenBank, including 99 to 100% nucleotide identity with previously characterized TCSV isolates from tomato, pepper, and jimsonweed in Puerto Rico (4). Consistent with the identification of TCSV, the known TCSV vector Frankliniella schultzei was identified in the lettuce with an adult population of 10 to 21 thrips per plant. Symptomatic lettuce leaves were used to mechanically inoculate 10-day-old lettuce and 56-day-old tobacco (Nicotiana tabacum) plants. Symptoms reminiscent of the original lettuce developed, and the presence of TCSV was confirmed by RT-PCR as described above. This is the first report of TCSV infection of lettuce in Puerto Rico and demonstrates that TCSV can be a limiting factor to lettuce production here and elsewhere in the Caribbean. References: (1) S. Adkins and E. N. Rosskopf. Plant Dis. 86:1310, 2002. (2) M. S. Silva et al. Arch. Virol. 146:1267, 2001. (3) C. G. Webster et al. Virology 413:216, 2011. (4) C. G. Webster et al. Plant Health Progress doi:10.1094/PHP-2013-0812-01-BR, 2013.

Sweet oranges in Puerto Rico and other citrus-producing areas in the world have been greatly affected by the Huanglongbing disease (HLB). Historically, most of Puerto Rico’s citrus production has been located from 0–600 m above sea level, where fruit acquire a desirable color and flavor due to climatic conditions. However, higher populations of the disease vector Diaphorina citri Kuwayama have been reported at these elevations in Puerto Rico. Although only 6.4% of the land area is located above 600 m, it is composed mainly of environmentally sensitive or non-arable land where Inceptisols are the dominant soil order. For that reason, ‘Marr’s Early’ and ‘Pera’ sweet orange (Citrus sinensis) scions grafted on ‘Carrizo’, ‘HRS 802’, and ‘HRS 812’ rootstocks were planted in Alonso clay series Inceptisol (Oxic Humudepts) at 649 m in 2010. Tree growth parameters (height, diameter, canopy volume) and yield efficiency were measured. Fruit quality was determined from juice content (%), total soluble solids [°Brix], and pH. Leaf tissue analyses showed an optimum range for Ca, Mg, Na, P, B, Cu, and Zn, an indicator of tree health. A few were high (i.e., N and P) or in excess (i.e., Fe), but no clear connection to specific scions or rootstocks could be established. Tree height, tree diameter, fruit production, and juice content were higher in both sweet oranges grafted on ‘HRS 802’ compared with those on ‘HRS 812’ and ‘Carrizo’. Therefore, ‘HRS 802’ rootstock can be recommended to local farmers growing sweet oranges in Alonso series soil.

Tomato chlorotic spot virus (TCSV) has recently been detected in tomato, bell pepper, jimsonweed, and lettuce in Puerto Rico. Observations of weeds and additional crops in 2015 and 2016 revealed TCSV-like symptoms. Testing of these symptomatic plants identified three new hosts of TCSV in Puerto Rico: erect spiderling (Boerhavia erecta); Asian spiderflower (Cleome viscosa); and sweet chili pepper (Capsicum chinense).

Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) se ha encontrado recientemente en todas las regiones productoras de frijol de Puerto Rico. Se observó que Megalurothrips usitatus afecta a las líneas de frijol andino (Phaseolus vulgaris L.), a Glycine max (L.) y también a Phaseolus acutifolius L. Esta es la primera vez que se reporta M. usitatus en Puerto Rico. Dado el alto riesgo que representa esta plaga para las leguminosas en Puerto Rico, es necesario evaluar qué zona de cultivo de habichuela y qué otros cultivos locales podrían verse más afectados.