Biogas plants are the most complex systems and are heavily studied in the field of renewable energy. A biogas system is mainly influenced by biological and technical parameters that strongly interact with each other. One recommended practice when operating a biogas plant is the recirculation of the substrate from the second fermenter into the first fermenter, which extends the recirculation amount (RA) and, in turn, the recirculation rate (RR). This technique should be applied to support and secure the biogas process. In this investigation, the RA was varied, starting with the recommended “best practice” of 10.0 m³/d (RR 40%). Every ten days, the RA was reduced in steps of 1.5 m³/d, with 5.5 m³/d (RR 27%) being the final value. The basic question to be addressed concerns to what extent the RR influences the methane yield and thereby influence the efficiency of a manure-based biogas plant in practice. Diverting the “best practice” to a RR of 27% stabilised the fermentation process and lead to significantly higher methane yields with smaller standard deviations. In addition, with a reduced RR, the standard optimal acid concentration within the biogas substrate was approximately reached.