The COVID-19 pandemic has led to a global disruption of several services, including routine immunizations. This effect has been described in several countries, but there are few detailed studies in Latin America and no reports in Ecuador. Therefore, this work aims to quantify the reduction in routine immunizations for infants during the 2020 COVID-19 pandemic in Ecuador. 2018, 2019, and 2020 data were obtained from the Ministry of Health, Ecuador. The number of doses and the extent of immunization coverage was descriptively compared for four vaccines: rotavirus (ROTA), poliovirus (PV), pneumococcal (PCV), and pentavalent (PENTA) vaccines. There was no significant difference in doses applied during the 2018 and 2019 years. However, a significant (p < 0.05) drop of 137,000 delivered doses was observed in 2020 compared to the pre-pandemic years. Reductions in the percentage of coverage were more pronounced for the PENTA vaccine (17.7%), followed by PV (16.4%), ROTA (12%), and PCV vaccines (10.7%). Spatial analysis shows a severe impact on vaccination coverage on provinces from the Coast and Highland regions of the country. The pandemic has significantly impacted the immunization programs for infants across Ecuador. This retrospective analysis shows an urgent need to protect vulnerable zones and populations during public health emergencies.

El agua subterránea es una de las principales fuentes de abastecimiento para los habitantes de las parroquias de Limoncocha y de Pompeya, ubicadas en la Amazonía ecuatoriana donde, además, se encuentra la Reserva Biológica Limoncocha (RBL) y su área de influencia (AI), cuyo ecosistema subterráneo recibe un aporte de agua proveniente de flujos externos a su límite. Tradicionalmente, el agua subterránea ha sido considerada protegida por el subsuelo, sin embargo, la disposición de contaminantes estables en zonas de infiltración favorable, podría causar que este recurso se contamine, poniendo en riesgo la salud de la población y la sostenibilidad de la reserva. Por ello, se propuso evaluar el peligro de contaminación del agua subterránea de dicha reserva, a través de la superposición de un mapa de vulnerabilidad del acuífero aplicando el método GODS, con un inventario de cargas contaminantes usando el método POSH y en función a esto, priorizar medidas en la protección de este recurso. Se determinó que, en la mayor parte del área de estudio, no existe peligro de contaminación del agua subterránea, no obstante, también se encontraron zonas de moderado y alto peligro para las que se propusieron medidas para prevenir el deterioro de la calidad del agua subterránea.

This research represents a study in an Amazonian village that has similar structures to others Kichwa Amazonian villages of Ecuador. We evaluated the diversity, ecology, possibility of mosquitoes/ pathogens translocation from forest to urban area, and the vulnerability by potential mosquito vectors of diseases through an intensive and fast method done January 2017. Our analyses registered a high diversity of mosquitos in Limoncocha village (33 spp, H’ 2.76), which includes four new records of species for Ecuador. We propose the biological reserve and the lagoon are determinant environmental factors for the high mosquito diversity, plus the socio-economic characteristics related with a deficient water pipeline supply and lack of solid waste system. Furthermore, the high diversity of sylvan mosquitoes registered throughout the area, that include several potential vectors, suggest a moderate to high vulnerability for the transference of pathogens from the Biological Reserve to the urbanized area, which may increase the circulation of little-known arboviruses (Mayaro, Ilheus, St Louis encephalitis) across Ecuador.

INTRODUCTION. Phytotelmata are a botanical group with water-impounding structures formed by non-aquatic plants, such as leaf axils, perforated internodes, stem holes or depressions, rot-holes in tree trunks or branches (tree-holes), flowers, open fruits, among others. These plants offer suitable conditions for aquatic or semi-aquatic animals, in particular arthropods. Immature mosquitoes (Diptera: Culicidae) are well represented among the inhabitants of phytotelmata. OBJECTIVE. Carry out a rapid assessment of the biodiversity of mosquitoes present in different land uses in the population and biological reserve of Limoncocha, to assess the vulnerability and risk of transmission of pathogens that cause emerging diseases. METHODS. Immature mosquitoes were collected in different phytotelmata including Ananas comosus leaf axils in a deforested area with small crops nearby of the Limoncocha Biological Reserve. RESULTS. Fourth instar larvae of Wyeomyia (Wyeomyia) medioalbipes Lutz were found in this plant to confirm their presence in the country and to the cultivated pineapples as phytotelmata in Ecuador. DISCUSSION AND CONCLUSIONS. This is the first report for Ecuador of Ananas comosus as phytotelmata and the associated mosquito species. Additionally, the presence of Wy. medioalpes in the country is confirmed. This information is added in the epidemiological evaluation of vulnerability and risk for the transmission of pathogens with a biogeographical and ecological vision.

This research presents a significant contribution to the methodologies and protocols for studying diatom communities in cryoconite holes on glaciers. Cryoconite holes are unique microenvironments found on glacial surfaces that support intricate microbial ecosystems, with diatoms playing a pivotal role in these communities. The refined methodologies developed in this study include optimizing sampling techniques to ensure that collections are both representative and diverse, which is crucial for accurate ecological assessments. Additionally, advanced digestion processes have been implemented to effectively isolate and purify diatom samples while minimizing contaminants, thereby improving sample integrity. Improved microscopic mounting techniques enhance visual clarity, facilitating more precise identifications of diatoms under the microscope. Furthermore, integrating DNA-based taxonomy broadens the taxonomic scope, providing valuable molecular insights into the diversity and evolutionary relationships of diatoms. Collectively, these protocols aim to enhance the reliability, depth, and multidimensional understanding of diatom ecology in cryoconite holes and broader glacial ecosystems, ultimately contributing to the field of glaciology and microbial ecology.