Background: Conventional nanoparticle synthesis methods involve harsh conditions, high costs, and environmental pollution. In this context, researchers are actively searching for sustainable, eco-friendly alternatives to conventional chemical synthesis methods. This has led to the development of green synthesis procedures among which the exploration of the plant-mediated synthesis of nanoparticles experienced a great development. Especially, because plant extracts can work as reducing and stabilizing agents. This opens up new possibilities for cost-effective, environmentally-friendly nanoparticle synthesis with enhanced size uniformity and stability. Moreover, bio-inspired nanoparticles derived from plants exhibit intriguing pharmacological properties, making them highly promising for use in medical applications due to their biocompatibility and nano-dimension. Objective: This study investigates the role of specific phytochemicals, such as phenolic compounds, terpenoids, and proteins, in plant-mediated nanoparticle synthesis together with their influence on particle size, stability, and properties. Additionally, we highlight the potential applications of these bio-derived nanoparticles, particularly with regard to drug delivery, disease management, agriculture, bioremediation, and application in other industries. Methodology: Extensive research on scientific databases identified green synthesis methods, specifically plant-mediated synthesis, with a focus on understanding the contributions of phytochemicals like phenolic compounds, terpenoids, and proteins. The database search covered the field’s development over the past 15 years. Results: Insights gained from this exploration highlight plant-mediated green synthesis for cost-effective nanoparticle production with significant pharmacological properties. Utilizing renewable biological resources and controlling nanoparticle characteristics through biomolecule interactions offer promising avenues for future research and applications. Conclusion: This review delves into the scientific intricacies of plant-mediated synthesis of nanoparticles, highlighting the advantages of this approach over the traditional chemical synthesis methods. The study showcases the immense potential of green synthesis for medical and other applications, aiming to inspire further research in this exciting area and promote a more sustainable future.

Cancer is a highly complex disease that affects lives and causes millions of deaths worldwide.

The increasing global energy demand and environmental concerns have highlighted the need for sustainable and renewable energy sources. Lignocellulosic biomass (LCB), rich in cellulose, hemicellulose, and lignin, is a promising resource for biofuel production. However, the recalcitrant nature of lignin poses a significant challenge by obstructing effective LCB decomposition. This review provides a comprehensive analysis of recent advancements in genetic and metabolic engineering techniques, focusing on directed and random mutagenesis to enhance cellulase production in fungi. It explores how these techniques can overcome challenges in lignin degradation and improve LCB conversion efficiency. Lignin's high resistance to degradation, due to its complex association with cellulose and hemicelluloses, necessitates the development of advanced fungal strains through mutagenesis. Fungi, which are efficient lignin degraders, benefit from these practices to enhance enzyme production and address environmental pollution from burning LCB. The review emphasizes engineering cellulolytic fungi through mutagenesis, gene-editing, and synthetic biology, highlighting CRISPR-Cas innovations and nanoparticle-based delivery systems for precise CRISPR-Cas application. It also discusses the role of transcription factors in boosting enzyme production and the practical applications of these techniques for in-situ LCB biodegradation. Effective implementation of these advancements could foster a sustainable economy and mitigate the negative environmental impacts of current agricultural practices.