The aim of the proposed study was to validate a rapid, uncomplicated, accurate, robust and sensitive bio-analytical method for quantifying asciminib using ivosidenib as an internal standard in rat plasma. This work provides a summary of recent advances in bio-analytical HPLC methodologies employing a Luna phenyl hexyl column (150 × 4.6 mm, 3.5) with an organic mobile phase of potassium dihydrogen phosphate (adjusted to pH 3.0 with orthophosphoric acid) and acetonitrile in the ratio 40:60. With a correlation coefficient of 0.999, the asciminib calibration curve was linear over the range of 100-2000 ng/mL. Using liquid-liquid extraction, the percent of recovered ASB ranged between 97.8 and 99.9%. The plasma stability of the bench-top and auto sampler remained stable for 24 h in the auto sampler. The T1/2 is 5 h and Tmax is 3 h, as calculated from the pharmacokinetic parameters. The application indicates that all parameters of system suitability, specificity, linearity and accuracy are in good agreement with the prescribed limits; consequently, the current method is highly stable, rapid and capable of analyzing plasma samples. This method is ideally adapted for determining asciminib in pure or dose form due to its novel technology.

Aim: The aim of this study was to simultaneously enhance the solubility and stability of bacogenins hydrolyzed bacoside rich extract by a ternary system comprised of hydrogenated soy lecithin and a third auxiliary substance, fulvic acid.Method: Both ternary and binary complexes were prepared using the solvent evaporation method were characterized by Fourier transform infrared technique, differential scanning calorimeter and scanning electron microscope. The entrapment efficacy in both binary and ternary system was calculated and the effect on the solubility, dissolution and stability of bacogenins was found out. Furthermore, the prepared complexes were subjected to behavioural pharmacological studies.Results : FTIR, DSC, and SEM studies in totality confirmed the formation of binary and ternary complexes. Enhancement in solubility was observed, and the order of release characteristics was found to be BHFS> BHSL>BHF> BH when the dissolution studies were carried out in 40% aqueous solution of ethanol. A significant improvement in the memory and antioxidant capacity was noticed in both binary, ternary complexes and fulvic acid treatment groups.Conclusion: The results revealed that the ternary complex could be a promising drug delivery system to improve the oral bioavailability of the bacogenins.

The aim of the project was to investigate Peat-derived Fulvic acid for its propensity to form co-crystals with quercetin and curcumin and characterize it by using different analytical techniques. The formation of co-crystals generally enhances water solubility and the overall bioavailability of molecules. Co-crystals were synthesized using a 1:1 stoichiometric ratio of fulvic acid with quercetin and curcumin, respectively, using solvent crystallization techniques taking tetrahydrofuran and water in a 1:1 v/v ratio. The co-crystals were characterized by spectroscopic methods, FTIR and Differential scanning calorimetry. Further confirmation was made by morphological studies using SEM. A structural analysis was also carried out, using ¹³C solid-state NMR analysis. The studies confirmed the formation of semi crystalline forms. Furthermore, the saturation solubility displayed the enhancement in solubility of up to 10, 5-folds for Quercetin and Curcumin, respectively. The in vitro dissolution results showed that T50% was achieved within 30 min for both the drugs. The literature supports that the nutraceutical co-crystals offer advantages, particularly in the improvement of biopharmaceutical properties and addressing the challenges of the lab and manufacturing scale process. Both the semi crystalline powders exhibited enhanced solubility and a better dissolution profile.