This page has only limited features, please log in for full access.
Penicillium expansum is an important postharvest pathogen of pomaceous fruit and a causal agent of blue mold or soft rot. In this study, we investigated the effect of ambient pH on growth, ultrastructure alteration, and pathogenicity of P. expansum, as well as accumulation of patulin and expression of genes involved in patulin biosynthesis. Under different pH, the fungus was routinely cultured and collected for growth, pathogenicity, patulin production, and gene expression studies using transmission electron microscopy, apple inoculation, HPLC, and RT-qPCR methods. Different ambient pH had significant impact on expression of genes and growth factors involved in patulin biosynthesis. Under same range of pH, gene expression profile, growth factors, and patulin accumulation (in vivo and in vitro) all showed similar changing trends. A well-developed cell was observed in addition to upregulation of genes at pH between pH 5.0 and 7.0, while the opposite was observed when pH was too basic (8.5) or too acid (2.5). Additionally, ambient pH had direct or indirect influence on expression of PecreaA, PelaeA, and PepacC. These findings will help in understanding the effect of ambient pH on growth, pathogenicity, and patulin production and support the development of successful methods for combating P. expansum infection on apple fruits.
Carelle Jimdjio; Huali Xue; Yang Bi; Mina Nan; Lan Li; Rui Zhang; Qili Liu; Lumei Pu. Effect of Ambient pH on Growth, Pathogenicity, and Patulin Production of Penicillium expansum. Toxins 2021, 13, 550 .
AMA StyleCarelle Jimdjio, Huali Xue, Yang Bi, Mina Nan, Lan Li, Rui Zhang, Qili Liu, Lumei Pu. Effect of Ambient pH on Growth, Pathogenicity, and Patulin Production of Penicillium expansum. Toxins. 2021; 13 (8):550.
Chicago/Turabian StyleCarelle Jimdjio; Huali Xue; Yang Bi; Mina Nan; Lan Li; Rui Zhang; Qili Liu; Lumei Pu. 2021. "Effect of Ambient pH on Growth, Pathogenicity, and Patulin Production of Penicillium expansum." Toxins 13, no. 8: 550.
A newly synthesized fluorescent chemosensor H6L was explored for detecting B4O72−, characterized by 1H NMR spectrum, mass spectrum and fluorescence spectra. During the detection process of B4O72−, the fluorescence is significantly enhanced and naked eye recognition can be performed under 365 nm UV light without any interference by other typical anions. The limit of detection is as low as 6.97 × 10-10 M. In addition, in order to broaden the application of salamo-based fluorescence sensors in the field of biology, except for the fluorescence imaging of HeLa cells, the first attempt of exogenous detection in zebrafish was conducted successfully.
Lu-Mei Pu; Lan Wang; Zhi-Li Wei; Zhuang-Zhuang Chen; Hai-Tao Long; Jia-Xi Ru; Xiu-Yan Dong. A newly synthesized visual bis(salamo)-based fluorescent chemosensor for exogenous detection of B4O72− in living cells and zebrafish. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2020, 249, 119263 .
AMA StyleLu-Mei Pu, Lan Wang, Zhi-Li Wei, Zhuang-Zhuang Chen, Hai-Tao Long, Jia-Xi Ru, Xiu-Yan Dong. A newly synthesized visual bis(salamo)-based fluorescent chemosensor for exogenous detection of B4O72− in living cells and zebrafish. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2020; 249 ():119263.
Chicago/Turabian StyleLu-Mei Pu; Lan Wang; Zhi-Li Wei; Zhuang-Zhuang Chen; Hai-Tao Long; Jia-Xi Ru; Xiu-Yan Dong. 2020. "A newly synthesized visual bis(salamo)-based fluorescent chemosensor for exogenous detection of B4O72− in living cells and zebrafish." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 249, no. : 119263.
Two newly designed tetranuclear transition metal(II) complexes, [{Co(L)(μ-OAc)Co(CH3OH)2}2] and [{Ni(L)(DMF)(μ-OAc)Ni}2] derived from an unsymmetrical salamo-like donor-N2O4 ligand (H3L: 6-hydroxy-6′-methoxy-2, 2′-[ethylenediyldioxybis(nitrilomethylidyne)]diphenol) were synthesized and characterized by elemental analysis, FT-IR, UV-vis, fluorescence spectroscopy, X-ray crystallography and Hirshfeld surface analysis. The Co(II) complex includes two fully deprotonated (L)3− moieties, two μ-acetato ligands and four coordinated methanol molecules, bearing a highly symmetrical tetranuclear structure. The terminal Co(II) ions are penta-coordinated and have slightly twisted triangular bipyramidal geometries, and the central Co(II) ions are hexa-coordinated and possess slightly twisted octahedral geometries. The Co(II) complex was self-assembled by adequate intermolecular hydrogen bonding interactions giving rise to an infinite two-dimensional supramolecular structure. The Ni(II) complex adopts a symmetric tetranuclear structure including two fully deprotonated (L)3− units, two coordinated DMF molecules, and two μ-acetato ligands. All the Ni(II) ions are hexa-coordinated and have slightly twisted octahedral geometries. At the same time, the fluorescent properties and Hirshfeld surfaces analyses of the Co(II) and Ni(II) complexes were also investigated in detail.
Lu-Mei Pu; Meng Yu; Yu Zhang; Hai-Tao Long; Wei-Bing Xu; Wen-Kui Dong. Tetranuclear cobalt(II) and nickel(II) complexes with an unsymmetrical salamo-like ligand: Structural characterization, Hirshfeld analysis and fluorescent properties. Inorganica Chimica Acta 2019, 500, 119238 .
AMA StyleLu-Mei Pu, Meng Yu, Yu Zhang, Hai-Tao Long, Wei-Bing Xu, Wen-Kui Dong. Tetranuclear cobalt(II) and nickel(II) complexes with an unsymmetrical salamo-like ligand: Structural characterization, Hirshfeld analysis and fluorescent properties. Inorganica Chimica Acta. 2019; 500 ():119238.
Chicago/Turabian StyleLu-Mei Pu; Meng Yu; Yu Zhang; Hai-Tao Long; Wei-Bing Xu; Wen-Kui Dong. 2019. "Tetranuclear cobalt(II) and nickel(II) complexes with an unsymmetrical salamo-like ligand: Structural characterization, Hirshfeld analysis and fluorescent properties." Inorganica Chimica Acta 500, no. : 119238.
A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%–104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.
Mina Nan; Yang Bi; Huali Xue; Sulin Xue; Haitao Long; Lumei Pu; Guorui Fu. Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly. Toxins 2019, 11, 71 .
AMA StyleMina Nan, Yang Bi, Huali Xue, Sulin Xue, Haitao Long, Lumei Pu, Guorui Fu. Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly. Toxins. 2019; 11 (2):71.
Chicago/Turabian StyleMina Nan; Yang Bi; Huali Xue; Sulin Xue; Haitao Long; Lumei Pu; Guorui Fu. 2019. "Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly." Toxins 11, no. 2: 71.
A highly selective fluorescent sensor H4L based on a bis(salamo)-type compound with two N2O2 chelating moieties as ionophore was successfully developed. Sensor H4L was found to have excellent selectivity for B4O72− over many other anions (Br−, CI−, CN−, CO32−, HCO3−, H2PO4−, HSO4−, NO3−, OAc−, S2O3−, SCN−, SO42−, Hcy (homocysteine) and H2O2), and it exhibited an approximately 150-fold enhancement of the fluorescence response to B4O72− in Tris-HCl buffer (DMF/H2O = 9:1, v/v, pH = 7) solutions. Significantly, its fluorescence intensity was enhanced in a linear fashion with increasing concentrations of B4O72−. The detection limit of sensor H4L towards B4O72− was 8.61 × 10−7 M. The test strips could conveniently, efficiently and simply detect B4O72− ions in Tris-HCl buffer (DMF/H2O = 9:1, v/v, pH = 7) solutions. Furthermore, sensor H4L showed excellent membrane permeability in living cells, and it was successfully used to monitor intracellular B4O72− by confocal luminescence imaging.
Lu-Mei Pu; Xiao-Yan Li; Jing Hao; Yin-Xia Sun; Yang Zhang; Hai-Tao Long; Wen-Kui Dong. Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B4O72− in water-containing systems and living cells. Scientific Reports 2018, 8, 1 -9.
AMA StyleLu-Mei Pu, Xiao-Yan Li, Jing Hao, Yin-Xia Sun, Yang Zhang, Hai-Tao Long, Wen-Kui Dong. Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B4O72− in water-containing systems and living cells. Scientific Reports. 2018; 8 (1):1-9.
Chicago/Turabian StyleLu-Mei Pu; Xiao-Yan Li; Jing Hao; Yin-Xia Sun; Yang Zhang; Hai-Tao Long; Wen-Kui Dong. 2018. "Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B4O72− in water-containing systems and living cells." Scientific Reports 8, no. 1: 1-9.
[Co2(L)Ce(OAc)3(CH3CH2OH)]·1.5CH3OH∙0.5CH2Cl2, a heterotrinuclear Co(II)-Ce(III) bis(salamo)-type complex with a symmetric bi(salamo)-type ligand H4L and an acyclic naphthalenediol moiety, was designed, synthesized and characterized by elemental analyses, FT-IR, UV-Vis and fluorescence spectroscopy and X-ray crystallography. The X-ray crystallographic investigation revealed the heterotrinuclear complex consisted of two Co(II) atoms, one Ce(III) atom, one (L)4‒ unit, three μ2-acetate ions, one coordinated ethanol molecule, one and half crystallization methanol molecule and half crystallization dichloromethane molecule. Two Co(II) atoms located in the N2O2 coordination spheres, are both hexacoordinated, with slightly distorted octahedral geometries. The Ce(III) atom is nine-coordinated and located in the O6 cavity possesses a single square antiprismatic geometry. In addition, supramolecular interactions exist in the Co(II)-Ce(III) complex. Two infinite 2D supramolecular structures are built via intermolecular O–H···O, C–H···O and C–H···π interactions, respectively.
Lu-Mei Pu; Qing Zhao; Ling-Zhi Liu; Han Zhang; Hai-Tao Long; Wen-Kui Dong. Synthesis and Fluorescence Properties of a New Heterotrinuclear Co(II)-Ce(III)Complex Constructed from a bis(salamo)-Type Tetraoxime Ligand. Molecules 2018, 23, 804 .
AMA StyleLu-Mei Pu, Qing Zhao, Ling-Zhi Liu, Han Zhang, Hai-Tao Long, Wen-Kui Dong. Synthesis and Fluorescence Properties of a New Heterotrinuclear Co(II)-Ce(III)Complex Constructed from a bis(salamo)-Type Tetraoxime Ligand. Molecules. 2018; 23 (4):804.
Chicago/Turabian StyleLu-Mei Pu; Qing Zhao; Ling-Zhi Liu; Han Zhang; Hai-Tao Long; Wen-Kui Dong. 2018. "Synthesis and Fluorescence Properties of a New Heterotrinuclear Co(II)-Ce(III)Complex Constructed from a bis(salamo)-Type Tetraoxime Ligand." Molecules 23, no. 4: 804.