Cross-view geo-localization (CVGL) involves determining the localization of aerial images by retrieving the most similar GPS-tagged satellite images. However, the imaging gaps between platforms are often significant and the variations in viewpoints are substantial, which limits the ability of existing methods to effectively associate cross-view features and extract consistent and invariant characteristics. Moreover, existing methods often overlook the problem of increased computational and storage requirements when improving model performance. To handle these limitations, we propose a lightweight enhanced alignment network, called the multilevel embedding and alignment network (MEAN). The MEAN framework uses a progressive multilevel enhancement strategy, global-to-local associations, and cross-domain alignment, enabling feature communication across levels. This allows MEAN to effectively connect features at different levels and learn robust cross-view consistent mappings and cross-view invariant features. Moreover, MEAN adopts a shallow backbone network combined with a lightweight branch design, effectively reducing parameter count and computational complexity. Experimental results on the University-1652 and SUES-200 datasets demonstrate that MEAN reduces parameter count by 62.17% and computational complexity by 70.99% compared with state-of-the-art models, while maintaining competitive or even superior performance. Our code is available at https://github.com/ISChenawei/MEAN

Background: Chronic kidney disease (CKD) and non-alcoholic fatty liver disease (NAFLD) are closely related to immune and inflammatory pathways. This study aimed to explore the diagnostic markers for CKD patients with NAFLD.Methods: CKD and NAFLD microarray data sets were screened from the GEO database and analyzed the differentially expressed genes (DEGs) in GSE10495 of CKD date set. Weighted Gene Co-Expression Network Analysis (WGCNA) method was used to construct gene coexpression networks and identify functional modules of NAFLD in GSE89632 date set. Then obtaining NAFLD-related share genes by intersecting DEGs of CKD and modular genes of NAFLD. Then functional enrichment analysis of NAFLD-related share genes was performed. The NAFLD-related hub genes come from intersection of cytoscape software and machine learning. ROC curves were used to examine the diagnostic value of NAFLD related hub genes in the CKD data sets and GSE89632 date set of NAFLD. CIBERSORTx was also used to explore the immune landscape in GSE104954, and the correlation between immune infiltration and hub genes expression was investigated.Results: A total of 45 NAFLD-related share genes were obtained, and 4 were NAFLD-related hub genes. Enrichment analysis showed that the NAFLD-related share genes were significantly enriched in immune-related pathways, programmed cell death, and inflammatory response. ROC curve confirmed 4 NAFLD-related hub genes in CKD training set GSE104954 and other validation sets. Then they were used as diagnostic markers for CKD. Interestingly, these 4 diagnostic markers of CKD also showed good diagnostic value in the NAFLD date set GSE89632, so these genes may be important targets of NAFLD in the development of CKD. The expression levels of the 4 diagnostic markers for CKD were significantly correlated with the infiltration of immune cells.Conclusion: 4 NAFLD-related genes (DUSP1, NR4A1, FOSB, ZFP36) were identified as diagnostic markers in CKD patients with NAFLD. Our study may provide diagnostic markers and therapeutic targets for CKD patients with NAFLD.

Non-Gaussian noises always exist in the nonlinear system, which usually lead to inconsistency and divergence of the regression and identification applications. The conventional evolving fuzzy systems (EFSs) in common sense have succeeded to conquer the uncertainties and external disturbance employing the specific variable structure characteristic. However, non-Gaussian noises would trigger the frequent changes of structure under the transient criteria, which severely degrades performance. Statistical criterion provides an informed choice of the strategies of the structure evolution, utilizing the approximation uncertainty as the observation of model sufficiency. The approximation uncertainty can be always decomposed into model uncertainty term and noise term, and is suitable for the non-Gaussian noise condition, especially relaxing the traditional Gaussian assumption. In this article, a novel incremental statistical evolving fuzzy inference system (SEFIS) is proposed, which has the capacity of updating the system parameters, and evolving the structure components to integrate new knowledge in the new process characteristic, system behavior, and operating conditions with non-Gaussian noises. The system generates a new rule based on the statistical model sufficiency which gives so insight into whether models are reliable and their approximations can be trusted. The nearest rule presents the inactive rule under the current data stream and further would be deleted without losing any information and accuracy of the subsequent trained models when the model sufficiency is satisfied. In our article, an adaptive maximum correntropy extend Kalman filter is derived to update the parameters of the evolving rules to cope with the non-Gaussian noises problems to further improve the robustness of parameter updating process. The parameter updating process shares an estimate of the uncertainty with the criteria of the structure evolving process to make the computation less of a burden dramatically. The simulation studies show that the proposed SEFIS has faster learning speed and is more accurate than the existing evolving fuzzy systems (EFSs) in the case of noise free and noisy conditions.

MicroRNAs exert regulatory effects on a number of genes, thereby contributing to both physiological and pathological processes. The functions of microRNAs in tumorigenesis are becoming increasingly clear. In the present study, we investigated the role of microRNA-125b (miR‑125b), previously implicated in prostate and breast cancer, in gastric cancer, particularly regarding proliferation and apoptosis of gastric cancer cells. The expression of miR‑125b was measured in 50 samples of gastric cancer tissues and corresponding para-cancerous tissues by real-time PCR. The levels of miR‑125b expression in the gastric cancer tissues were significantly higher compared to the adjacent normal tissues (P<0.05). To begin to understand how the increased expression of miR‑125b may promote gastric cancer, the miR‑125b mimic was transfected into the gastric cancer cell line, HGC‑27, for the determination of proliferation (CCK8) and apoptosis (Annexin V) by flow cytometry. The results demonstrated that the proliferation significantly increased and apoptosis significantly decreased in the HGC‑27 cells following transfection with the miR‑125b mimic, compared to the untreated and scramble‑treated controls (P<0.05). Thus, miR‑125b may act as an oncogene in gastric cancer by dysregulating gastric cell proliferation and apoptosis.

In this paper, a novel self-evolving data cloud-based PID-like controller (SEDCPID) is proposed for uncertain nonlinear systems. The proposed SEDCPID controller is constructed by using fuzzy rules with non-parametric data cloud-based antecedence and PID-like consequence. The antecedent data clouds adopt the relative data density to represent the fuzzy firing strength of input variables instead of the explicit design of the membership functions in the classical sense. The proposed SEDCPID controller has the advantages of evolving structure and adapting parameter concurrently in an online manner. The density and distance information of data clouds are proposed to achieve the addition and deletion of data clouds and also a stable recursive method is proposed to update the parameters of the PID-like sub-controllers for the fast convergence performance. Based on the Lyapunov stability theory, the stability of the proposed controller is proven and the proof shows the tracking errors converge to a small neighborhood. Numerical and experimental results illustrate the effectiveness of the proposed controller in handling the uncertain nonlinear dynamic systems.