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In the present work, a rapid, accurate and cost-effective method has been developed for the simultaneous quantification of phenolic compounds in oil using mixed-mode solid-phase extraction (SPE) coupled with chemical labeling UHPLC-MS/MS. Mix-mode SPE weak cation cartridges were selected to enrich and purify phenolic compounds in oil, and hydroxyl moiety was dansylation as stable-isotope internal standard. The major parameters that affected the extraction and chemical labeling efficiency were investigated, and the method was fully validated. The limit of quantifications and the limit of detections were 0.002 µg kg−1 ~ 0.10 µg kg−1 and 0.006 µg kg−1 ~ 0.30 µg kg−1, respectively. The recoveries were 61.2% ~ 129.3% with intra-day and inter-day precision less than 12%. The results for 38 rapeseed oils revealed that 14 phenolic compounds, including canolol, phenolic acids, phenolic alcohols, tyrosol and vanillin from trace levels to relatively high content.
Xu Yu; Li Yu; Fei Ma; Peiwu Li. Quantification of phenolic compounds in vegetable oils by mixed-mode solid-phase extraction isotope chemical labeling coupled with UHPLC-MS/MS. Food Chemistry 2020, 334, 127572 .
AMA StyleXu Yu, Li Yu, Fei Ma, Peiwu Li. Quantification of phenolic compounds in vegetable oils by mixed-mode solid-phase extraction isotope chemical labeling coupled with UHPLC-MS/MS. Food Chemistry. 2020; 334 ():127572.
Chicago/Turabian StyleXu Yu; Li Yu; Fei Ma; Peiwu Li. 2020. "Quantification of phenolic compounds in vegetable oils by mixed-mode solid-phase extraction isotope chemical labeling coupled with UHPLC-MS/MS." Food Chemistry 334, no. : 127572.
Graphene oxide wrapped silica nanocomposites were synthesized and selected as solid phase extraction adsorbents for high performance liquid chromatography analysis of aflatoxins. The major parameters affecting extraction efficiency were optimized, including the amount of adsorbents, extraction time and desorption conditions. The limit of detections and the limit of quantifications were from 0.1 to 0.3 µg/kg and from 0.3 to 1.0 µg/kg, respectively. The recoveries of aflatoxins in the spiked maize and rice samples were in the range of 76.8-104.7% and 81.1-106.9%, respectively, and with the RSDs less than 12.4%. The proposed method was proven to be simple, rapid and reliable for routine analysis of aflatoxins in crops.
Li Yu; Fei Ma; Xiaoxia Ding; Hengling Wang; Peiwu Li. Silica/graphene oxide nanocomposites: Potential adsorbents for solid phase extraction of trace aflatoxins in cereal crops coupled with high performance liquid chromatography. Food Chemistry 2018, 245, 1018 -1024.
AMA StyleLi Yu, Fei Ma, Xiaoxia Ding, Hengling Wang, Peiwu Li. Silica/graphene oxide nanocomposites: Potential adsorbents for solid phase extraction of trace aflatoxins in cereal crops coupled with high performance liquid chromatography. Food Chemistry. 2018; 245 ():1018-1024.
Chicago/Turabian StyleLi Yu; Fei Ma; Xiaoxia Ding; Hengling Wang; Peiwu Li. 2018. "Silica/graphene oxide nanocomposites: Potential adsorbents for solid phase extraction of trace aflatoxins in cereal crops coupled with high performance liquid chromatography." Food Chemistry 245, no. : 1018-1024.
Adulteration of edible oils has attracted attention from more researchers and consumers in recent years. Complex multispecies adulteration is a commonly used strategy to mask the traditional adulteration detection methods. Most of the researchers were only concerned about single targeted adulterants, however, it was difficult to identify complex multispecies adulteration or untargeted adulterants. To detect adulteration of edible oil, identification of characteristic markers of adulterants was proposed to be an effective method, which could provide a solution for multispecies adulteration detection. In this study, a simple method of multispecies adulteration detection for camellia oil (adulterated with soybean oil, peanut oil, rapeseed oil) was developed by quantifying chemical markers including four isoflavones, trans-resveratrol and sinapic acid, which used liquid chromatography tandem mass spectrometry (LC-MS/MS) combined with solid phase extraction (SPE). In commercial camellia oil, only two of them were detected of daidzin with the average content of 0.06 ng/g while other markers were absent. The developed method was highly sensitive as the limits of detection (LODs) ranged from 0.02 ng/mL to 0.16 ng/mL and the mean recoveries ranged from 79.7% to 113.5%, indicating that this method was reliable to detect potential characteristic markers in edible oils. Six target compounds for pure camellia oils, soybean oils, peanut oils and rapeseed oils had been analyzed to get the results. The validation results indicated that this simple and rapid method was successfully employed to determine multispecies adulteration of camellia oil adulterated with soybean, peanut and rapeseed oils.
Xinjing Dou; Jin Mao; Liangxiao Zhang; Huali Xie; Lin Chen; Li Yu; Fei Ma; Xiupin Wang; Qi Zhang; Peiwu Li. Multispecies Adulteration Detection of Camellia Oil by Chemical Markers. Molecules 2018, 23, 241 .
AMA StyleXinjing Dou, Jin Mao, Liangxiao Zhang, Huali Xie, Lin Chen, Li Yu, Fei Ma, Xiupin Wang, Qi Zhang, Peiwu Li. Multispecies Adulteration Detection of Camellia Oil by Chemical Markers. Molecules. 2018; 23 (2):241.
Chicago/Turabian StyleXinjing Dou; Jin Mao; Liangxiao Zhang; Huali Xie; Lin Chen; Li Yu; Fei Ma; Xiupin Wang; Qi Zhang; Peiwu Li. 2018. "Multispecies Adulteration Detection of Camellia Oil by Chemical Markers." Molecules 23, no. 2: 241.
Capsaicin and dihydrocapsaicin were selected as adulteration markers to authenticate vegetable oils. In this study, a method of immunoaffinity chromatography (IAC) combined with liquid chromatography-tandem mass spectrometry was established for the determination of capsaicin and dihydrocapsaicin in vegetable oils. In this method, immunosorbents were obtained by covalently coupling highly specific capsaicinoid polyclonal antibodieswith CNBr-activated Sepharose 4B, and then packed into a polyethylene column. In this paper, the major parameters affecting IAC extraction efficiency, including loading, washing and eluting conditions, were also investigated. The IAC column displayed high selectivity for capsaicin and dihydrocapsaicin with the maximum capacity of 240ng. The limit of detection (LOD) and limit of quantification (LOQ) for capsaicin were calculated as 0.02 and 0.08μgkg(-1), and for dihydrocapsaicin were 0.03 and 0.10μgkg(-1). The recoveries of capsaicin and dihydrocapsaicin in oil samples were in the range of 87.3-95.2% with the relative standard deviation (RSD) of less than 6.1%. The results indicated that capsaicinoid compounds could not be found in edible vegetable oils. Therefore, the proposed method is simple, reliable and adequate for routine monitoring of capsaicinoid compounds in vegetable oils and has an excellent potential for detection of adulteration with inedible waste oil.
Fei Ma; Qingqing Yang; Bertrand Matthäus; Peiwu Li; Qi Zhang; Liangxiao Zhang. Simultaneous determination of capsaicin and dihydrocapsaicin for vegetable oil adulteration by immunoaffinity chromatography cleanup coupled with LC–MS/MS. Journal of Chromatography B 2016, 1021, 137 -144.
AMA StyleFei Ma, Qingqing Yang, Bertrand Matthäus, Peiwu Li, Qi Zhang, Liangxiao Zhang. Simultaneous determination of capsaicin and dihydrocapsaicin for vegetable oil adulteration by immunoaffinity chromatography cleanup coupled with LC–MS/MS. Journal of Chromatography B. 2016; 1021 ():137-144.
Chicago/Turabian StyleFei Ma; Qingqing Yang; Bertrand Matthäus; Peiwu Li; Qi Zhang; Liangxiao Zhang. 2016. "Simultaneous determination of capsaicin and dihydrocapsaicin for vegetable oil adulteration by immunoaffinity chromatography cleanup coupled with LC–MS/MS." Journal of Chromatography B 1021, no. : 137-144.
A novel magnetic carboxylated multi-walled carbon nanotubes (c-MWCNT-MNPs) was proposed for magnetic solid-phase extraction coupled with liquid chromatography–tandem mass spectrometry to determine phenolic compounds in sesame oil. In this study, c-MWCNT-MNPs were acquired by simply dispersing Fe3O4 magnetic nanoparticles into carboxylated multi-walled carbon nanotubes. The major parameters affecting extraction efficiency were optimized, including the type and volume of desorption solvents, extraction and desorption time, washing solution, and sorbent amount. The limit of quantifications and limit of detections were from 0.03 μg/kg to 43.00 μg/kg and from 0.01 μg/kg to 13.60 μg/kg, respectively. The recoveries of phenolic compounds in vegetable oils were in the range of 83.8–125.9% with inter-day and intra-day precisions of less than 13.2%. It was confirmed that this method was simple, rapid and reliable with an excellent potential for routine analysis of phenolic compounds in oil samples.
Rao Wu; Fei Ma; Liangxiao Zhang; Peiwu Li; Guangming Li; Qi Zhang; Wen Zhang; Xiuping Wang. Simultaneous determination of phenolic compounds in sesame oil using LC–MS/MS combined with magnetic carboxylated multi-walled carbon nanotubes. Food Chemistry 2016, 204, 334 -342.
AMA StyleRao Wu, Fei Ma, Liangxiao Zhang, Peiwu Li, Guangming Li, Qi Zhang, Wen Zhang, Xiuping Wang. Simultaneous determination of phenolic compounds in sesame oil using LC–MS/MS combined with magnetic carboxylated multi-walled carbon nanotubes. Food Chemistry. 2016; 204 ():334-342.
Chicago/Turabian StyleRao Wu; Fei Ma; Liangxiao Zhang; Peiwu Li; Guangming Li; Qi Zhang; Wen Zhang; Xiuping Wang. 2016. "Simultaneous determination of phenolic compounds in sesame oil using LC–MS/MS combined with magnetic carboxylated multi-walled carbon nanotubes." Food Chemistry 204, no. : 334-342.
To ensure authenticity of vegetable oils, isoflavones (genistein, genistin, daidzein and daidzin) and resveratrols (cis-resveratrol and trans-resveratrol) were selected as the putative markers for adulteration of soybean and peanut oils. Firstly, mixed mode solid-phase extraction coupled with liquid chromatography tandem mass spectrometry (mixed-mode SPE LC–MS/MS) method was developed to analyze isoflavones and resveratrols in vegetable oils. The concentration of marker compounds in vegetable oils were 0.08–1.47 mg kg−1 for daidzein, ND-78.9 μg kg−1 for daidzin, 0.40–5.89 mg kg−1 for genistein, 1.2–114.9 μg kg−1 for genistin, 3.1–85.0 μg kg−1 for trans-resveratrol and 1.9–51.0 μg kg−1 for cis-resveratrol, which are compatible with the raw materials for oil press. Additionally, the applicability of this method has been successfully tested in thirteen vegetable oils from the market. Mixed-mode SPE LC–MS/MS method can simultaneously detect isoflavones and resveratrols in vegetable oils and assess adulteration and quality of soybean and peanut oils.
Xin Zhao; Fei Ma; Peiwu Li; Guangming Li; Liangxiao Zhang; Qi Zhang; Wen Zhang; Xiupin Wang. Simultaneous determination of isoflavones and resveratrols for adulteration detection of soybean and peanut oils by mixed-mode SPE LC–MS/MS. Food Chemistry 2015, 176, 465 -471.
AMA StyleXin Zhao, Fei Ma, Peiwu Li, Guangming Li, Liangxiao Zhang, Qi Zhang, Wen Zhang, Xiupin Wang. Simultaneous determination of isoflavones and resveratrols for adulteration detection of soybean and peanut oils by mixed-mode SPE LC–MS/MS. Food Chemistry. 2015; 176 ():465-471.
Chicago/Turabian StyleXin Zhao; Fei Ma; Peiwu Li; Guangming Li; Liangxiao Zhang; Qi Zhang; Wen Zhang; Xiupin Wang. 2015. "Simultaneous determination of isoflavones and resveratrols for adulteration detection of soybean and peanut oils by mixed-mode SPE LC–MS/MS." Food Chemistry 176, no. : 465-471.
This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G1, B1, G2 and B2 were 0.03, 0.07, 0.05 and 0.09 μg·kg−1, and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg−1, respectively. The recoveries of aflatoxins in samples were 90.1%–104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg−1.
Fei Ma; Ran Chen; Peiwu Li; Qi Zhang; Wen Zhang; Xiaofeng Hu. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules 2013, 18, 2222 -2235.
AMA StyleFei Ma, Ran Chen, Peiwu Li, Qi Zhang, Wen Zhang, Xiaofeng Hu. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules. 2013; 18 (2):2222-2235.
Chicago/Turabian StyleFei Ma; Ran Chen; Peiwu Li; Qi Zhang; Wen Zhang; Xiaofeng Hu. 2013. "Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products." Molecules 18, no. 2: 2222-2235.
By introducing long carbon-chain alkyl groups at the C-13 position of berberine and palmatine, 13-n-hexyl/13-n-octyl berberine and palmatine chloride analogues 4a–d were synthesized and examined by MTT assays for cytotoxic activity in seven human cancer cell lines (7701QGY, SMMC7721, HepG2, CEM, CEM/VCR, KIII, Lewis), yielding IC50 values of 0.02 ± 0.01–13.58 ± 2.84 μM. 13-n-Octyl palmatine (compound 4d) gave the most potent inhibitor activity, with an IC50 of 0.02 ± 0.01 μM for SMMC7721. In all cases, the 13-n-alkyl berberine and palmatine analogues 4a–d were more cytotoxic than berberine and palmatine. In addition, compounds 4a–d also exhibited more potent cytotoxicity than berberine and palmatine in mice with S180 sarcoma xenografted in vivo. The primary screening results indicated that the 13-n-hexyl/13-n-octyl berberine and palmatine analogues might be valuable source for new potent anticancer drug candidates.
Lei Zhang; Jingjing Li; Fei Ma; Shining Yao; Naisan Li; Jing Wang; Yongbin Wang; Xiuzhen Wang; Qizheng Yao. Synthesis and Cytotoxicity Evaluation of 13-n-Alkyl Berberine and Palmatine Analogues as Anticancer Agents. Molecules 2012, 17, 11294 -11302.
AMA StyleLei Zhang, Jingjing Li, Fei Ma, Shining Yao, Naisan Li, Jing Wang, Yongbin Wang, Xiuzhen Wang, Qizheng Yao. Synthesis and Cytotoxicity Evaluation of 13-n-Alkyl Berberine and Palmatine Analogues as Anticancer Agents. Molecules. 2012; 17 (10):11294-11302.
Chicago/Turabian StyleLei Zhang; Jingjing Li; Fei Ma; Shining Yao; Naisan Li; Jing Wang; Yongbin Wang; Xiuzhen Wang; Qizheng Yao. 2012. "Synthesis and Cytotoxicity Evaluation of 13-n-Alkyl Berberine and Palmatine Analogues as Anticancer Agents." Molecules 17, no. 10: 11294-11302.