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Wen Zhang
Quality Inspection & Test Center for Oilseed Products, Ministry of Agriculture, Wuhan 430062, China

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Journal article
Published: 29 October 2020 in Toxins
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Ochratoxin A (OTA) is a known food contaminant that affects a wide range of food and agricultural products. The presence of this fungal metabolite in foods poses a threat to human health. Therefore, various detection and quantification methods have been developed to determine its presence in foods. Herein, we describe a rapid and ultrasensitive tracer-based fluorescence polarization immunoassay (FPIA) for the detection of OTA in rice samples. Four fluorescent tracers OTA-fluorescein thiocarbamoyl ethylenediamine (EDF), OTA-fluorescein thiocarbamoyl butane diamine (BDF), OTA-amino-methyl fluorescein (AMF), and OTA-fluorescein thiocarbamoyl hexame (HDF) with fluorescence polarization values (δFP = FPbind-FPfree) of 5, 100, 207, and 80 mP, respectively, were synthesized. The tracer with the highest δFP value (OTA-AMF) was selected and further optimized for the development of an ultrasensitive FPIA with a detection range of 0.03–0.78 ng/mL. A mean recovery of 70.0% to 110.0% was obtained from spiked rice samples with a relative standard deviation of equal to or less than 20%. Good correlations (r2 = 0.9966) were observed between OTA levels in contaminated rice samples obtained by the FPIA method and high-performance liquid chromatography (HPLC) as a reference method. The rapidity of the method was confirmed by analyzing ten rice samples that were analyzed within 25 min, on average. The sensitivity, accuracy, and rapidity of the method show that it is suitable for screening and quantification of OTA in food samples without the cumbersome pre-analytical steps required in other mycotoxin detection methods.

ACS Style

Xiaorong Huang; Xiaoqian Tang; Abdoulie Jallow; Xin Qi; Wen Zhang; Jun Jiang; Hui Li; Qi Zhang; Peiwu Li. Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice. Toxins 2020, 12, 682 .

AMA Style

Xiaorong Huang, Xiaoqian Tang, Abdoulie Jallow, Xin Qi, Wen Zhang, Jun Jiang, Hui Li, Qi Zhang, Peiwu Li. Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice. Toxins. 2020; 12 (11):682.

Chicago/Turabian Style

Xiaorong Huang; Xiaoqian Tang; Abdoulie Jallow; Xin Qi; Wen Zhang; Jun Jiang; Hui Li; Qi Zhang; Peiwu Li. 2020. "Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice." Toxins 12, no. 11: 682.

Journal article
Published: 23 April 2020 in Toxins
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Anti-idiotypic nanobodies, usually expressed by gene engineering protocol, has been shown as a nontoxic coating antigen for toxic compound immunoassays. We here focused on how to increase immunoassay sensitivity by changing the nanobody’s primary sequence. In the experiments, two anti-idiotype nanobodies against monoclonal antibody 1H2, which is specific to ochratoxin A, were obtained and named as nontoxic coating antigen 1 (NCA1) and nontoxic coating antigen 2 (NCA2). Three differences between the nanobodies were discovered. First, there are six amino acid residues (AAR) of changes in the complementarity determining region (CDR), which compose the antigen-binding site. One of them locates in CDR1 (I–L), two of them in CDR2 (G–D, E–K), and three of them in CDR3 (Y–H, Y–W). Second, the affinity constant of NCA1 was tested as 1.20 × 108 L mol−1, which is about 4 times lower than that of NCA2 (5.36 × 108 L mol−1). Third, the sensitivity (50% inhibition concentration) of NCA1 for OTA was shown as 0.052 ng mL−1, which was 3.5 times lower than that of nontoxic coating antigen 2 (0.015 ng mL−1). The results indicate that the AAR changes in CDR of the anti-idiotypic nanobodies, from nonpolar to polar, increasing the affinity constant may enhance the immunoassay sensitivity. In addition, by using the nontoxic coating antigen 2 to substitute the routine synthetic toxic antigen, we established an eco-friendly and green enzyme-linked immunosorbent assay (ELISA) method for rapid detection of ochratoxin A in cereals. The half-maximal inhibitory concentration (IC50) of optimized ELISA was 0.017 ng mL−1 with a limit of detection (LOD) of 0.003 ng mL−1. The optimized immunoassay showed that the average recoveries of spiked corn, rice, and wheat were between 80% and 114.8%, with the relative standard deviation (RSD) ranging from 3.1–12.3%. Therefore, we provided not only basic knowledge on how to improve the structure of anti-idiotype nanobody for increasing assay sensitivity, but also an available eco-friendly ELISA for ochratoxin A in cereals.

ACS Style

Caixia Zhang; Weiqi Zhang; Xiaoqian Tang; Qi Zhang; Wen Zhang; Peiwu Li. Change of Amino Acid Residues in Idiotypic Nanobodies Enhanced the Sensitivity of Competitive Enzyme Immunoassay for Mycotoxin Ochratoxin A in Cereals. Toxins 2020, 12, 273 .

AMA Style

Caixia Zhang, Weiqi Zhang, Xiaoqian Tang, Qi Zhang, Wen Zhang, Peiwu Li. Change of Amino Acid Residues in Idiotypic Nanobodies Enhanced the Sensitivity of Competitive Enzyme Immunoassay for Mycotoxin Ochratoxin A in Cereals. Toxins. 2020; 12 (4):273.

Chicago/Turabian Style

Caixia Zhang; Weiqi Zhang; Xiaoqian Tang; Qi Zhang; Wen Zhang; Peiwu Li. 2020. "Change of Amino Acid Residues in Idiotypic Nanobodies Enhanced the Sensitivity of Competitive Enzyme Immunoassay for Mycotoxin Ochratoxin A in Cereals." Toxins 12, no. 4: 273.

Review
Published: 01 January 2020 in Toxins
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Aflatoxin contamination has been causing great concern worldwide due to the major economic impact on crop production and their toxicological effects to human and animals. Contamination can occur in the field, during transportation, and also in storage. Post-harvest contamination usually derives from the pre-harvest infection of aflatoxigenic molds, especially aflatoxin-producing Aspergilli such as Aspergillus flavus and A. parasiticus. Many strategies preventing aflatoxigenic molds from entering food and feed chains have been reported, among which biological control is becoming one of the most praised strategies. The objective of this article is to review the biocontrol strategy for inhibiting the growth of and aflatoxin production by aflatoxigenic fungi. This review focuses on comparing inhibitory behaviors of different antagonistic microorganisms including various bacteria, fungi and yeasts. We also reviewed the bioactive compounds produced by microorganisms and the mechanisms leading to inhibition. The key factors influencing antifungal activities of antagonists are also discussed in this review.

ACS Style

Xianfeng Ren; Qi Zhang; Wen Zhang; Jin Mao; Peiwu Li. Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors. Toxins 2020, 12, 24 .

AMA Style

Xianfeng Ren, Qi Zhang, Wen Zhang, Jin Mao, Peiwu Li. Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors. Toxins. 2020; 12 (1):24.

Chicago/Turabian Style

Xianfeng Ren; Qi Zhang; Wen Zhang; Jin Mao; Peiwu Li. 2020. "Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors." Toxins 12, no. 1: 24.

Journal article
Published: 09 August 2019 in Foods
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Phytosterols are important micronutrients in human diets. Evidence has shown that phytosterols play an essential role in the reduction of cholesterol in blood and therefore decrease cardiovascular morbidity. In this study, the content and composition of phytosterols in different kinds of vegetable oils were analyzed, and the total phytosterol intake and contribution of foods to intake were estimated based on consumption data. The results showed that the phytosterol contents of rice bran oil, corn oil, and rapeseed oil were higher than those of other vegetable oils and the intake of phytosterol in the Chinese diet was about 392.3 mg/day. The main sources of phytosterols were edible vegetable oils (46.3%), followed by cereals (38.9%), vegetables (9.2%), nuts (2.0%), fruits (1.5%), beans and bean products (1.4%), and tubers (0.8%). Among all vegetable oils, rapeseed oil was the main individual contributor to phytosterol intake (22.9%), especially for the southern residents of China.

ACS Style

Ruinan Yang; Li Xue; Liangxiao Zhang; Xuefang Wang; Xin Qi; Jun Jiang; Li Yu; Xiupin Wang; Wen Zhang; Qi Zhang; Peiwu Li. Phytosterol Contents of Edible Oils and Their Contributions to Estimated Phytosterol Intake in the Chinese Diet. Foods 2019, 8, 334 .

AMA Style

Ruinan Yang, Li Xue, Liangxiao Zhang, Xuefang Wang, Xin Qi, Jun Jiang, Li Yu, Xiupin Wang, Wen Zhang, Qi Zhang, Peiwu Li. Phytosterol Contents of Edible Oils and Their Contributions to Estimated Phytosterol Intake in the Chinese Diet. Foods. 2019; 8 (8):334.

Chicago/Turabian Style

Ruinan Yang; Li Xue; Liangxiao Zhang; Xuefang Wang; Xin Qi; Jun Jiang; Li Yu; Xiupin Wang; Wen Zhang; Qi Zhang; Peiwu Li. 2019. "Phytosterol Contents of Edible Oils and Their Contributions to Estimated Phytosterol Intake in the Chinese Diet." Foods 8, no. 8: 334.

Journal article
Published: 01 August 2019 in Metabolites
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Rapeseed is an important oilseed with proper fatty acid composition and abundant bioactive components. Canada and China are the two major rapeseed-producing countries all over the world. Meanwhile, Canada and Mongolia are major importers of rapeseed due to the great demand for rapeseed in China. To investigate the metabolites in rapeseeds from three countries, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics was employed to analyze rapeseeds from China, Canada, and Mongolia. As results, 67, 53, and 68 metabolites showed significant differences between Chinese and Canadian, Chinese and Mongolian, and Canadian and Mongolian rapeseeds, respectively. Differential metabolites were mainly distributed in the metabolic pathways including phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, and ubiquinone and other terpenoid-quinone biosynthesis. Among the differential metabolites, contents of sinapate and sinapine were higher in Chinese rapeseeds, while the contents of brassicasterol, stigmasterol, and campestanol were higher in Canadian rapeseeds. These findings might provide insight into the metabolic characteristics of rapeseeds from three countries to guide processing and consumption of the products of rapeseed.

ACS Style

Ruinan Yang; Ligang Deng; Liangxiao Zhang; Xiaofeng Yue; Jin Mao; Fei Ma; Xiupin Wang; Qi Zhang; Wen Zhang; Peiwu Li. Comparative Metabolomic Analysis of Rapeseeds from Three Countries. Metabolites 2019, 9, 161 .

AMA Style

Ruinan Yang, Ligang Deng, Liangxiao Zhang, Xiaofeng Yue, Jin Mao, Fei Ma, Xiupin Wang, Qi Zhang, Wen Zhang, Peiwu Li. Comparative Metabolomic Analysis of Rapeseeds from Three Countries. Metabolites. 2019; 9 (8):161.

Chicago/Turabian Style

Ruinan Yang; Ligang Deng; Liangxiao Zhang; Xiaofeng Yue; Jin Mao; Fei Ma; Xiupin Wang; Qi Zhang; Wen Zhang; Peiwu Li. 2019. "Comparative Metabolomic Analysis of Rapeseeds from Three Countries." Metabolites 9, no. 8: 161.

Journal article
Published: 11 February 2019 in Toxins
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Deoxynivalenol (DON) is a secondary metabolite produced by Fusarium, which is a trichothecene mycotoxin. As the main mycotoxin with high toxicity, wheat, barley, corn and their products are susceptible to contamination of DON. Due to the stability of this mycotoxin, traditional methods for DON reduction often require a strong oxidant, high temperature and high pressure with more energy consumption. Therefore, exploring green, efficient and environmentally friendly ways to degrade or reduce DON is a meaningful and challenging issue. Herein, a dendritic-like α-Fe2O3 was successfully prepared using a facile hydrothermal synthesis method at 160 °C, which was systematically characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). It was found that dendritic-like α-Fe2O3 showed superior activity for the photocatalytic degradation of DON in aqueous solution under visible light irradiation (λ > 420 nm) and 90.3% DON (initial concentration of 4.0 μg/mL) could be reduced in 2 h. Most of all, the main possible intermediate products were proposed through high performance liquid chromatography-mass spectrometry (HPLC-MS) after the photocatalytic treatment. This work not only provides a green and promising way to mitigate mycotoxin contamination but also may present useful information for future studies.

ACS Style

Huiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins 2019, 11, 105 .

AMA Style

Huiting Wang, Jin Mao, Zhaowei Zhang, Qi Zhang, Liangxiao Zhang, Peiwu Li, Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins. 2019; 11 (2):105.

Chicago/Turabian Style

Huiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. 2019. "Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation." Toxins 11, no. 2: 105.

Journal article
Published: 20 January 2019 in Toxins
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Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B1 (AFB1), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 µg/kg in maize and 0.18, 0.57, and 1.47 µg/kg in peanut with an analytical range of 0.48–20, 1.56–200, and 3.63–300 µg/kg for AFB1, ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24–110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety.

ACS Style

Du Wang; Jianguo Zhu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Jun Jiang; Xiaomei Chen; Xuefang Wang; Peiwu Li. Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation. Toxins 2019, 11, 56 .

AMA Style

Du Wang, Jianguo Zhu, Zhaowei Zhang, Qi Zhang, Wen Zhang, Li Yu, Jun Jiang, Xiaomei Chen, Xuefang Wang, Peiwu Li. Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation. Toxins. 2019; 11 (1):56.

Chicago/Turabian Style

Du Wang; Jianguo Zhu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Jun Jiang; Xiaomei Chen; Xuefang Wang; Peiwu Li. 2019. "Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation." Toxins 11, no. 1: 56.

Journal article
Published: 17 February 2018 in Toxins
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Multiple-mycotoxin contamination has been frequently found in the agro-food monitoring due to the coexistence of fungi. However, many determination methods focused on a single mycotoxin, highlighting the demand for on-site determination of multiple mycotoxins in a single run. We develop a multicolor-based immunochromatographic strip (ICS) for simultaneous determination of aflatoxin B1 (AFB1), zearalenone (ZEN) and T-2 toxin in maize- and cereal-based animal feeds. The nanoparticles with different colors are conjugated with three monoclonal antibodies, which serve as the immunoassay probes. The decrease in color intensity is observed by the naked eyes, providing simultaneous quantification of three mycotoxins. The visible limits of detection for AFB1, ZEN and T-2 are estimated to be 0.5, 2, and 30 ng/mL, respectively. The cut-off values are 1, 10, and 50 ng/mL, respectively. Considerable specificity and stability are found using real samples. The results are in excellent agreement with those from high-performance liquid chromatography/tandem mass spectrometry. The multi-color ICS boasts sensitive and rapid visual differentiation and simultaneous semi-quantification of aflatoxin B1, zearalenone and T-2 toxin in maize- and cereal-based feed samples within 20 min.

ACS Style

Lin Xu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Du Wang; Hui Li; Peiwu Li. An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay. Toxins 2018, 10, 87 .

AMA Style

Lin Xu, Zhaowei Zhang, Qi Zhang, Wen Zhang, Li Yu, Du Wang, Hui Li, Peiwu Li. An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay. Toxins. 2018; 10 (2):87.

Chicago/Turabian Style

Lin Xu; Zhaowei Zhang; Qi Zhang; Wen Zhang; Li Yu; Du Wang; Hui Li; Peiwu Li. 2018. "An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay." Toxins 10, no. 2: 87.

Journal article
Published: 13 October 2017 in Toxins
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A highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and strong absorption in the whole ultraviolet-visible (UV-Vis) range. The strong coordination interaction between nitrogen functional groups of the AFM1 aptamer and PdNPs brought FAM and PdNPs in close proximity, which resulted in the fluorescence quenching of FAM to a maximum extent of 95%. The non-specific fluorescence quenching caused by PdNPs towards fluorescein was negligible. After the introduction of AFM1 into the FAM-AFM1 aptamer-PdNPs FRET system, the AFM1 aptamer preferentially combined with AFM1 accompanied by conformational change, which greatly weakened the coordination interaction between the AFM1 aptamer and PdNPs. Thus, fluorescence recovery of FAM was observed and a linear relationship between the fluorescence recovery and the concentration of AFM1 was obtained in the range of 5–150 pg/mL in aqueous buffer with the detection limit of 1.5 pg/mL. AFM1 detection was also realized in milk samples with a linear detection range from 6 pg/mL to 150 pg/mL. The highly sensitive FRET aptasensor with simple configuration shows promising prospect in detecting a variety of food contaminants.

ACS Style

Hui Li; Daibin Yang; Peiwu Li; Qi Zhang; Wen Zhang; Xiaoxia Ding; Jin Mao; Jing Wu. Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk. Toxins 2017, 9, 318 .

AMA Style

Hui Li, Daibin Yang, Peiwu Li, Qi Zhang, Wen Zhang, Xiaoxia Ding, Jin Mao, Jing Wu. Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk. Toxins. 2017; 9 (10):318.

Chicago/Turabian Style

Hui Li; Daibin Yang; Peiwu Li; Qi Zhang; Wen Zhang; Xiaoxia Ding; Jin Mao; Jing Wu. 2017. "Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk." Toxins 9, no. 10: 318.

Journal article
Published: 19 May 2017 in Toxins
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Research about antibody specificity spectra was conducted to develop single-specific antibodies or broad-specific antibodies. Aflatoxins, as one class of high-toxicity mycotoxins, were selected as the research targets to investigate the effect of the immunogen dose on antibody specificity spectra. For this aim, 16 monoclonal antibodies were induced by low or high doses of aflatoxin B1-BSA, and 34 monoclonal antibodies were induced by low or high doses of aflatoxin M1-BSA. The specificities of the antibodies induced, whether by aflatoxin B1 conjugate or aflatoxin M1 conjugate, indicated that the low dose of the immunogen induced a narrow spectrum of antibody specificity, while the high dose of the immunogen showed an advantage to form a broad spectrum of antibody specificity. Therefore, this report provides important information for the development of new antibodies against small molecules like aflatoxins.

ACS Style

Peiwu Li; Jing Wu; Li Zhang; Zhiyong Fan; Tingting Yu; Feng Jiang; Xiaoqian Tang; Zhaowei Zhang; Wen Zhang; Qi Zhang. Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin. Toxins 2017, 9, 172 .

AMA Style

Peiwu Li, Jing Wu, Li Zhang, Zhiyong Fan, Tingting Yu, Feng Jiang, Xiaoqian Tang, Zhaowei Zhang, Wen Zhang, Qi Zhang. Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin. Toxins. 2017; 9 (5):172.

Chicago/Turabian Style

Peiwu Li; Jing Wu; Li Zhang; Zhiyong Fan; Tingting Yu; Feng Jiang; Xiaoqian Tang; Zhaowei Zhang; Wen Zhang; Qi Zhang. 2017. "Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin." Toxins 9, no. 5: 172.

Journal article
Published: 13 April 2017 in Toxins
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An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAbAFT) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAbAFT. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out durations were within 10 min. The recoveries for AFT in rice and peanut sample matrices were recorded from 86.25% to 118.0%, with relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety.

ACS Style

Suiyan Ouyang; Zhaowei Zhang; Ting He; Peiwu Li; Qi Zhang; Xiaomei Chen; Du Wang; Hui Li; Xiaoqian Tang; Wen Zhang. An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip. Toxins 2017, 9, 137 .

AMA Style

Suiyan Ouyang, Zhaowei Zhang, Ting He, Peiwu Li, Qi Zhang, Xiaomei Chen, Du Wang, Hui Li, Xiaoqian Tang, Wen Zhang. An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip. Toxins. 2017; 9 (4):137.

Chicago/Turabian Style

Suiyan Ouyang; Zhaowei Zhang; Ting He; Peiwu Li; Qi Zhang; Xiaomei Chen; Du Wang; Hui Li; Xiaoqian Tang; Wen Zhang. 2017. "An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip." Toxins 9, no. 4: 137.

Journal article
Published: 12 November 2016 in Toxins
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Aflatoxins, a group of extremely hazardous compounds because of their genotoxicity and carcinogenicity to human and animals, are commonly found in many tropical and subtropical regions. Ultraviolet (UV) irradiation is proven to be an effective method to reduce or detoxify aflatoxins. However, the degradation products of aflatoxins under UV irradiation and their safety or toxicity have not been clear in practical production such as edible oil industry. In this study, the degradation products of aflatoxin B1 (AFB1) in peanut oil were analyzed by Ultra Performance Liquid Chromatograph-Thermo Quadrupole Exactive Focus mass spectrometry/mass spectrometry (UPLC-TQEF-MS/MS). The high-resolution mass spectra reflected that two main products were formed after the modification of a double bond in the terminal furan ring and the fracture of the lactone ring, while the small molecules especially nitrogen-containing compound may have participated in the photochemical reaction. According to the above results, the possible photodegradation pathway of AFB1 in peanut oil is proposed. Moreover, the human embryo hepatocytes viability assay indicated that the cell toxicity of degradation products after UV irradiation was much lower than that of AFB1, which could be attributed to the breakage of toxicological sites. These findings can provide new information for metabolic pathways and the hazard assessment of AFB1 using UV detoxification.

ACS Style

Jin Mao; Bing He; Liangxiao Zhang; Peiwu Li; Qi Zhang; Xiaoxia Ding; Wen Zhang. A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B1 in Peanut Oil under UV Irradiation. Toxins 2016, 8, 332 .

AMA Style

Jin Mao, Bing He, Liangxiao Zhang, Peiwu Li, Qi Zhang, Xiaoxia Ding, Wen Zhang. A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B1 in Peanut Oil under UV Irradiation. Toxins. 2016; 8 (11):332.

Chicago/Turabian Style

Jin Mao; Bing He; Liangxiao Zhang; Peiwu Li; Qi Zhang; Xiaoxia Ding; Wen Zhang. 2016. "A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B1 in Peanut Oil under UV Irradiation." Toxins 8, no. 11: 332.

Journal article
Published: 14 July 2016 in Sensors
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Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3–10.0 µg·kg−1, with a limit of detection (LOD) of 0.1 µg·kg−1 and recoveries of 87.2%–114.3%, within 10 min. The results showed good correlation (R2 > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg−1. The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.

ACS Style

Du Wang; Zhaowei Zhang; Peiwu Li; Qi Zhang; Wen Zhang. Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis. Sensors 2016, 16, 1094 .

AMA Style

Du Wang, Zhaowei Zhang, Peiwu Li, Qi Zhang, Wen Zhang. Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis. Sensors. 2016; 16 (7):1094.

Chicago/Turabian Style

Du Wang; Zhaowei Zhang; Peiwu Li; Qi Zhang; Wen Zhang. 2016. "Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis." Sensors 16, no. 7: 1094.

Journal article
Published: 28 December 2015 in Toxins
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To detect and monitor G-group aflatoxins in agricultural products, we generated class-specific monoclonal antibodies that specifically recognized aflatoxins G1 and G2. Of the final three positive and stable hybridomas obtained, clone 2G6 produced a monoclonal antibody that had equal sensitivity to aflatoxins G1 and G2, and did not cross-react with aflatoxins B1, B2, or M1. Its IC50 values for aflatoxins G1 and G2 were 17.18 ng·mL−1 and 19.75 ng·mL−1, respectively. Using this new monoclonal antibody, we developed a competitive indirect enzyme-linked immunosorbent assay (CI-ELISA); the method had a limit of detection of 0.06 ng·mL−1. To validate this CI-ELISA, we spiked uncontaminated peanut samples with various amounts of aflatoxins G1 and G2 and compared recovery rates with those determined by a standard HPLC method. The recovery rates of the CI-ELISA ranging from 94% to 103% were comparable to those of the HPLC (92% to 102%). We also used both methods to determine the amounts of G-group aflatoxins in five peanut samples contaminated by aflatoxin B1-positive, and their relative standard deviations ranged from 8.4% to 17.7% (under 20%), which demonstrates a good correlation between the two methods. We further used this CI-ELISA to assess the ability of 126 fungal strains isolated from peanuts or field soils to produce G-group aflatoxins. Among these, seven stains producing different amounts of G-group aflatoxins were identified. Our results showed that the monoclonal antibody 2 G6-based CI-ELISA was suitable for the detection of G-group aflatoxins present in peanuts and also those produced by fungi.

ACS Style

Peiwu Li; Qian Zhou; Ting Wang; Haiyan Zhou; Wen Zhang; Xiaoxia Ding; Zhaowei Zhang; Perng-Kuang Chang; Qi Zhang. Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins. Toxins 2015, 8, 5 .

AMA Style

Peiwu Li, Qian Zhou, Ting Wang, Haiyan Zhou, Wen Zhang, Xiaoxia Ding, Zhaowei Zhang, Perng-Kuang Chang, Qi Zhang. Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins. Toxins. 2015; 8 (1):5.

Chicago/Turabian Style

Peiwu Li; Qian Zhou; Ting Wang; Haiyan Zhou; Wen Zhang; Xiaoxia Ding; Zhaowei Zhang; Perng-Kuang Chang; Qi Zhang. 2015. "Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins." Toxins 8, no. 1: 5.

Short communication
Published: 19 December 2015 in Chemometrics and Intelligent Laboratory Systems
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Highly predictive multivariate calibration model depends on samples in training set. In this study, we introduced an outlier detection method and developed its improvement for shorter run time. Improved Monte-Carlo outlier detection (IMCOD) was proposed to establish cross-prediction models for determining normal samples, which were subsequently used to analyze the distribution of prediction errors for all of dubious samples together. Four real datasets were employed to illustrate and validate the performance of IMCOD. After sample selection for training set of NIR of soy flour samples, the Root Mean Square Error of Prediction (RMSEP) of PLS model decreased from 1.4811 to 0.7650. This method benefits the establishment of a good model for QSAR and NIR datasets.

ACS Style

Liangxiao Zhang; Du Wang; Rongrong Gao; Peiwu Li; Wen Zhang; Jin Mao; Li Yu; Xiaoxia Ding; Qi Zhang. Improvement on enhanced Monte-Carlo outlier detection method. Chemometrics and Intelligent Laboratory Systems 2015, 151, 89 -94.

AMA Style

Liangxiao Zhang, Du Wang, Rongrong Gao, Peiwu Li, Wen Zhang, Jin Mao, Li Yu, Xiaoxia Ding, Qi Zhang. Improvement on enhanced Monte-Carlo outlier detection method. Chemometrics and Intelligent Laboratory Systems. 2015; 151 ():89-94.

Chicago/Turabian Style

Liangxiao Zhang; Du Wang; Rongrong Gao; Peiwu Li; Wen Zhang; Jin Mao; Li Yu; Xiaoxia Ding; Qi Zhang. 2015. "Improvement on enhanced Monte-Carlo outlier detection method." Chemometrics and Intelligent Laboratory Systems 151, no. : 89-94.

Journal article
Published: 11 February 2013 in Molecules
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This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G1, B1, G2 and B2 were 0.03, 0.07, 0.05 and 0.09 μg·kg−1, and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg−1, respectively. The recoveries of aflatoxins in samples were 90.1%–104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg−1.

ACS Style

Fei Ma; Ran Chen; Peiwu Li; Qi Zhang; Wen Zhang; Xiaofeng Hu. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules 2013, 18, 2222 -2235.

AMA Style

Fei Ma, Ran Chen, Peiwu Li, Qi Zhang, Wen Zhang, Xiaofeng Hu. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules. 2013; 18 (2):2222-2235.

Chicago/Turabian Style

Fei Ma; Ran Chen; Peiwu Li; Qi Zhang; Wen Zhang; Xiaofeng Hu. 2013. "Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products." Molecules 18, no. 2: 2222-2235.

Review
Published: 05 July 2012 in Sensors
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Chemical contaminants in food have caused serious health issues in both humans and animals. Microarray technology is an advanced technique suitable for the analysis of chemical contaminates. In particular, immuno-microarray approach is one of the most promising methods for chemical contaminants analysis. The use of microarrays for the analysis of chemical contaminants is the subject of this review. Fabrication strategies and detection methods for chemical contaminants are discussed in detail. Application to the analysis of mycotoxins, biotoxins, pesticide residues, and pharmaceutical residues is also described. Finally, future challenges and opportunities are discussed.

ACS Style

Zhaowei Zhang; Peiwu Li; Xiaofeng Hu; Qi Zhang; Xiaoxia Ding; Wen Zhang. Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects. Sensors 2012, 12, 9234 -9252.

AMA Style

Zhaowei Zhang, Peiwu Li, Xiaofeng Hu, Qi Zhang, Xiaoxia Ding, Wen Zhang. Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects. Sensors. 2012; 12 (7):9234-9252.

Chicago/Turabian Style

Zhaowei Zhang; Peiwu Li; Xiaofeng Hu; Qi Zhang; Xiaoxia Ding; Wen Zhang. 2012. "Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects." Sensors 12, no. 7: 9234-9252.

Book chapter
Published: 21 October 2011 in Aflatoxins - Detection, Measurement and Control
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ACS Style

Peiwu Li; Qi Zhang; Daohong Zhang; Di Guan; Xiaoxia; Ding Xuefen; Sufang Fang; Xiupin Wang; Wen Zhang. Aflatoxin Measurement and Analysis. Aflatoxins - Detection, Measurement and Control 2011, 1 .

AMA Style

Peiwu Li, Qi Zhang, Daohong Zhang, Di Guan, Xiaoxia, Ding Xuefen, Sufang Fang, Xiupin Wang, Wen Zhang. Aflatoxin Measurement and Analysis. Aflatoxins - Detection, Measurement and Control. 2011; ():1.

Chicago/Turabian Style

Peiwu Li; Qi Zhang; Daohong Zhang; Di Guan; Xiaoxia; Ding Xuefen; Sufang Fang; Xiupin Wang; Wen Zhang. 2011. "Aflatoxin Measurement and Analysis." Aflatoxins - Detection, Measurement and Control , no. : 1.

Journal article
Published: 04 January 2010 in Molecules
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Five generic haptens of pyrethoid insecticides, which were classified as three types, were designed and synthesized: the first (hapten 1) is for type I pyrethroids without a cyano group, the second (hapten 2 and XQ) for type II pyrethroids with a cyano group, and the third (hapten 4 and 5) for both types of pyrethroids with loss of the ester group. The hapten structures were confirmed by MS and 1H-NMR. Hapten 1 and 2 were conjugated with BSA respectively and haptens 1-5 were conjugated with OVA. Four polyclonal antisera were raised against BSA conjugates including a mixture conjugate, and twenty antibody/coating conjugate combinations were selected for studies of assay sensitivity and specificity for pyrethroids. The study revealed the best combination, which showed equal high sensitivities (I50 is around 0.02 μg mL-1) to both types of pyrethroids. The immunity results suggest that, with a mixture conjugates, a polyclonal antibody against a group of insecticides can be prepared for multi-residue assays.

ACS Style

Qi Zhang; Wen Zhang; Xiuping Wang; Peiwu Li. Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples. Molecules 2010, 15, 164 -177.

AMA Style

Qi Zhang, Wen Zhang, Xiuping Wang, Peiwu Li. Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples. Molecules. 2010; 15 (1):164-177.

Chicago/Turabian Style

Qi Zhang; Wen Zhang; Xiuping Wang; Peiwu Li. 2010. "Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples." Molecules 15, no. 1: 164-177.