Apple leaf spot caused by the Alternaria alternata f. sp. mali (ALT1) fungus is one of the most devastating diseases of apple (Malus × domestica). We identified a hairpin RNA (hpRNA) named MdhpRNA277 that produces small RNAs and is induced by ALT1 infection in 9Golden Delicious9 apple. MdhpRNA277 produces mdm-siR277-1 and mdm-siR277-2, which target five resistance (R) genes that are expressed at high levels in resistant apple variety 9Hanfu9 and at low levels in susceptible variety 9Golden Delicious9 following ALT1 infection. MdhpRNA277 was strongly induced in 9Golden Delicious9 but not 9Hanfu9 following ALT1 inoculation. MdhpRNA277 promoter activity was much stronger in inoculated 9Golden Delicious9 vs. 9Hanfu9. We identified a single nucleotide polymorphism (SNP) in the MdhpRNA277 promoter region between 9Golden Delicious9 (pMdhpRNA277-GD) and 9Hanfu9 (pMdhpRNA277-HF). The transcription factor MdWHy binds to pMdhpRNA277-GD, but not to pMdhpRNA277-HF. Transgenic 9GL-39 apple expressing pMdhpRNA277-GD:MdhpRNA277 was more susceptible to ALT1 infection than plants expressing pMdhpRNA277-HF:MdhpRNA277 due to induced mdm-siR277 accumulation and reduced expression of the five target R genes. We confirmed that the SNP in pMdhpRNA277 is associated with Alternaria leaf spot resistance by crossing. This SNP could be used as a marker to distinguish between apple varieties that are resistant or susceptible to Alternaria leaf spot.

Genetic diversity is associated with invasion dynamics during establishment and expansion stages by affecting the viability and adaptive potential of exotics. There have been many reports on the comparison between the genetic diversity of invasive alien species (IAS) in and out of their native habitats, but the conclusions were usually inconsistent. In this work, a standard meta-analysis of the genetic diversity of 19 invasive plants based on 26 previous studies was carried out to investigate the general trend for the change of IASs’ genetic diversity during their invasion process and its real correlation with the invasion fate. Those 26 studies were screened from a total of 3557 peer-reviewed publications from the ISI Web of Science database during the period of January 2000 to May 2022. Based on the selected studies in this work, a general reduction of IASs’ genetic diversity was found in non-native populations compared to that in native ones, while the difference was not significant. This finding suggested that regardless of the change in genetic diversity, it had no substantial effect on the outcome of the invasion process. Therefore, genetic diversity might not serve as a reliable indicator for risk assessment and prediction of invasion dynamic prediction in the case of IASs.

Dendrobium officinale (D. officinale) is a widely used traditional Chinese medicine with high economic value. MicroR159 (miR159) is an ancient and conserved microRNA (miRNA) family in land plants, playing roles in the progress of growth and development, as well as the stress response. In order to find out functions of miR159 in D. officinale, multiple bioinformatic approaches were employed and 10 MIR159 genes were found, localizing on seven chromosomes and an unanchored segment of the D. officinale genome. All of the precursor sequences of Dof-miR159 could form a stable stem-loop structure. The phylogenetic analysis revealed that the MIR159 genes of D. officinale were divided into five clades. Furthermore, the conservation analysis suggested that the 2 to 20 nt region of miR159 mature sequences were highly conserved among family members. The promoter analysis of MIR159s showed that the majority of the predicted cis-elements were related to environmental stress or hormones. In total, five classes of genes were predicted to be the target genes of Dof-miR159s, including GAMYB transcription factors, which had been confirmed in many other land plants. The expression patterns of predicted target genes revealed their potential roles in the growth and development of D. officinale, as well as in cold and drought stress responses. In conclusion, our results illustrated the stress-related miR159-targeted genes in D. officinale, which could provide candidate genes for resistance breeding in the future.

Dendrobium officinale Kimura et Migo is a perennial epiphytic herb in traditional Chinese medicine, showing remarkable resistance to salt stress. Water-soluble sugars serve as important osmoprotectants and play crucial roles in plant stress responses. Previous studies have primarily focused on sugar metabolism in individual tissues under stress, resulting in a limited understanding of the regulatory differences between tissues and the relationship between sugar metabolism and transport. A variety of salt-responsive genes were identified through transcriptome analysis of D. officinale. GO and KEGG enrichment analyses revealed functional differences among the differentially expressed genes (DEGs) between leaves and roots. Expression analysis indicated that sugar metabolic genes and D. officinale Sugars Will Eventually be Exported Transporters (DoSWEETs) displayed distinct expression patterns in leaves and roots under salt stress. Most sugar metabolic genes were up-regulated in the leaves and down-regulated in the roots in response to salt, while DoSWEETs predominantly responded in the roots. Specifically, DoSWEET2a, 6a, 12a, 14, and 16 were confirmed via RT-qPCR. Additionally, positive correlations were observed between certain genes (scrK, INV, SUS) and DoSWEETs, with INV (LOC110096666) showing a strong positive correlation with all detected DoSWEETs in both leaves and roots. Our findings not only illustrated the distinct responses of leaves and roots to salt stress, but also highlighted the relationship between sugar metabolic genes and DoSWEETs in adapting to such stress. This enhances our understanding of the differential responses of plant tissues to salt stress and identified candidate genes for salt-resistance breeding in D. officinale.