Androgenetic Alopecia (AGA) occurs due to over-response to androgens causing severe hair loss on the scalp, and requires the development of new and efficient drugs to treat this condition. This study explores and identifies secondary metabolites from Sansevieriatrifasciata Prain using the LC-MS/MS and in-silico method. The inhibitory activity of bioactive compounds from S. trifasciata Prain against androgen receptors (PDB ID: 4K7A) was evaluated molecularly using docking and dynamics studies by comparing their binding energies, interactions, and stability with minoxidil. The results of the LC-MS/MS analysis identified Methyl pyrophaeophorbide A (1), Oliveramine (2), (2S)-3′, 4′-Methylenedioxy-5, 7-dimethoxyflavane (3), 1-Acetyl-β-carboline (4), Digiprolactone (5), Trichosanic acid (6) and Methyl gallate (7) from the leaves subfraction of this plant. Three alkaloid compounds (compounds 1, 3, and 4), and one flavonoid (compound 2), had lower docking scores of −7.0, −5.8, −5.2, and −6.3 kcal/mol, respectively. The prediction of binding energy using the MM-PBSA approach ensured that the potency of the four compounds was better than minoxidil, with energies of −66.13, −59.36, −40.39, and −40.25 kJ/mol for compounds 1, 3, 2, and 4, respectively. The dynamics simulation shows the stability of compound 1 based on the trajectory analysis for the 100 ns simulation. This research succeeded in identifying the compound and assessing the anti-alopecia activity of Sansevieria trifasciata Prain. Seven compounds were identified as new compounds never reported in Sansevieria trifasciata Prain. Four compounds were predicted to have better anti-alopecia activity than minoxidil in inhibiting androgen receptors through an in silico approach.

Lansium domesticum Coor. is a fruit species from the Meliaceae family, which is a tropical plant native to Southeast Asia. Local citizens call it langsat, longkong, or duku and have used it as traditional medicine. The seeds of L. domesticum are used as a fever medicine, its bark is used to treat scorpion sting, and its leaves are used to repel mosquitoes. Because of its various uses, it is necessary to explore the antiradical potential of L. domesticum seeds. This study aims to determine the antiradical potential of L. domesticum seeds extract and fractions by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and to discover compounds that act as antiradical. Lansium domesticum seed powder was macerated with methanol, and then the extract was concentrated using a rotary evaporator and fractionated by n-hexane and ethyl acetate. The antiradical assay was conducted on extract and fractions by using DPPH radicals. Phenolic and flavonoid contents from extract and fractions were also tested. The ethyl acetate fraction obtained strong antiradical potential with an IC50 value of 8.938 � 0.031 �g/mL. Total phenolic and flavonoid contents of ethyl acetate fraction were higher with values ??of 58.25 � 0.501 mgGAE/g sample and 75.123 � 0.175 mgQE/g sample, respectively. Correlation of phenolic and flavonoid contents, which inhibited radicals had R2 values ??of 0.9182 and 0.7658. Ethyl acetate fraction of L. domesticum seeds had very strong antiradical activity. Further isolation is expected to be conducted to discover which compounds are the most responsible as antiradical.

Sansevieria trifasciata Prain holds great potential as a valuable asset in pharmaceutical development. In this study, our focus is to explore and assess the antibacterial activity of various components derived from this plant, including extracts, fractions, subfractions, and isolates, explicitly targeting two common bacteria: Escherichia coli and Streptococcus aureus. The isolated compound, identified as a derivative pyridone alkaloid (5-methyl-11-(2-oxopyridin-1(2H)-yl)undecaneperoxoicacid), demonstrates notable antibacterial effects. The extracts, fractions, subfractions, and isolates reveal significant bacterial growth reductions (p < 0.05). The minimum inhibitory concentration (MIC) values for Escherichia coli were 1.95 ppm, 3.9 ppm, 15.62 ppm, and 7.81 ppm, respectively, while the MIC values for Streptococcus aureus were 1.95 ppm, 1.95 ppm, 15.62 ppm, and 7.81 ppm, respectively. Computational analysis showed the isolates’ interaction with key residues on the active site of β-ketoacyl-ACP synthase from Escherichia coli and TyrRS from Streptococcus aureus. The findings indicate that the isolates exhibit a strong affinity for specific residues, including His333, Cys163, and Phe392 in β-ketoacyl-ACP synthase, as well as Arg88, His117, Glu160, and Gln213 in TyrRS. Comparative energy calculations using MMPBSA demonstrate the isolates’ favorable binding energy (−104,101 kJ/mol for β-ketoacyl-ACP synthase and −81,060 kJ/mol for TyrRS) compared to ciprofloxacin. The elucidated antibacterial activity and molecular interactions of the isolates present valuable knowledge for future in vitro studies, facilitating the development of novel antibacterial agents targeting diverse bacterial strains.

Androgenetic Alopecia (AGA) causes more than 90% of alopecia cases. Drugs for the treatment of AGA have toxic and side effects, so it is necessary to find new drugs from the natural product, such as Sansevieria trifasciata. This study aims to determine the hair growth activity of subfraction from Sansevieria trifasciata and characterize the isolate using infrared spectroscopy. The fraction of Sansevieria trifasciata was separated using column chromatography. The hair growth activity using the modified Tanaka method. Skin biopsies of each rabbit stained with hematoxylin and eosin. The characterization of the isolate using infrared spectroscopy (FT-IR). The separation of fractions obtained six subfractions. The hair growth activity from subfraction-C was higher with the hair growth length of 2.80 cm ± 0.00 (p

Ketersediaan obat merupakan komitmen pemerintah dalam melaksanakan pelayanan kesehatan. Ketersediaan obat di rumah sakit menentukan tercapainya pelayanan kesehatan yang optimal. Ketersediaan obat yang optimal adalah ketersediaan obat yang mencukupi kebutuhan bagi pasien. Penelitian ini dilakukan untuk mengevaluasi ketersediaan obat pada era JKN-BPJS Kesehatan di RSUD Kota Kendari Tahun 2015 dengan menggunakan indikator persentase nilai obat kedaluwarsa, persentase stok mati, persentase stok obat kosong (stock out), nilai TOR, dan tingkat ketersediaan obat. Penelitian ini merupakan penelitian deskriptif non eksperimental dengan pengumpulan data secara retrospektif. Data diperoleh dari pengamatan dan observasi dokumen tahun 2015 serta didukung dengan wawancara dengan Kepala Gudang Farmasi dan petugas gudang obat RSUD Kota Kendari. Hasil penelitian menunjukkan bahwa ketersediaan obat di RSUD Kota Kendari pada era JKN-BPJS tahun 2015 belum efisien dengan hasil persentase nilai obat kedaluwarsa sebesar 0,47%, persentase stok mati sebesar 2,27%, persentase stock out 15,07%, nilai TOR sebesar 8,02 kali dan tingkat ketersediaan obat 18,04 bulan. Kata kunci: ketersediaan obat, BPJS, rumah sakit, manajemen, Kendari

Context: The development of novel and potent alpha-glucosidase inhibitors from natural sources holds immense promise for treating diabetes. Aims: To identify the isolated compound, determine its inhibitory potency, explore its molecular interactions with the alpha-glucosidase receptor, and understand the role of specific structural features in its inhibitory activity. Methods: Research began with the isolation and identification of the new gallic acid derivative of the Schleichera oleosa leaf extract. The isolated compound (1) was characterized as 3,5-dihydroxy-4-methoxybenzoic acid using spectroscopic techniques. In vitro studies were carried out to compare the IC50 values of the ethanolic extract, isolate 1, and acarbose. Results: Isolate 1 exhibited significantly higher inhibitory potency against the -glucosidase enzyme compared to acarbose. Subsequently, molecular docking studies were performed to gain insight into the molecular interactions between the gallic acid derivatives and the active site of the -glucosidase receptor. The docking simulations revealed that the protection of the methyl group on the hydroxy group of the gallic acid derivatives played a crucial role in enhancing its binding affinity to the alpha-glucosidase receptor. This molecular interaction insight provides valuable information for the design of more potent and selective -glucosidase inhibitors based on this structural feature. Conclusions: These results collectively suggest that the newly isolated gallic acid derivative has promising potential as a natural -glucosidase inhibitor for diabetes management. Further research and optimization of this compound could lead to the development of novel and effective therapeutics for glycemic control in individuals with diabetes.