Royal jelly is an essential substance for the development of bees from larval to adult stages. Studies have identified a group of key proteins in royal jelly, denominated major royal jelly proteins (MRJPs). The group currently consists of nine proteins (MRJP1–MRJP9), with MRJP1 being the most abundant and MRJP3 being used as a microsatellite marker for the selection of queens with a greater production of royal jelly. The diet of bees is mostly composed of proteins, and supplementing this intake to encourage a higher production of their primary product is important for producers. It is estimated that, by adding probiotic and prebiotic organisms to their diets, the benefits to bees will be even greater, both for their immune systems and primary responses to stress. Circumstances that are adverse compared to those of the natural habitat of bees eventually substantially interfere with bee behavior. Stress situations are modulated by proteins termed heat shock proteins (HSPs). Among these, HSP70 has been shown to exhibit abundance changes whenever bees experience unusual situations of stress. Thus, we sought to supplement A. mellifera bee colony diets with proteins and prebiotic and probiotic components, and to evaluate the expression levels of MRJP3 and HSP70 mRNAs using qRT-PCR. The results revealed that differences in the expression of MRJP3 can be attributed to the different types of feed offered. Significant differences were evident when comparing the expression levels of MRJP3 and HSP70, suggesting that protein supplementation with pre/probiotics promotes positive results in royal jelly synthesis carried out by honey bee nurses.

In the light of the connectivism theory, the objective was to analyze the perception of use and satisfaction of accounting students about Edpuzzle, a digital video application. This tool was employed in four accounting courses using the cell phones of 92 students. A questionnaire was administered on-site and was structured in two parts. The first contained questions about the respondent's profile, and the second about the use and satisfaction with Edpuzzle. The results indicated that students liked to use Edpuzzle and felt satisfied, especially those under 21 years old. We also noticed significant correlations among the questions. For example, the positive association between interactivity and appropriateness of use of the Edpuzzle stands out. It is concluded, therefore, that the results corroborate the connectivism theory, which sustains that knowledge must suffer updates as the environment changes and that sharing is crucial for the creation of networks that feed each other. Edpuzzle helps in this regard.

Potencial fisiológico de sementes de soja submetidas ao tratamento industrial com bioestimulante antes e após armazenamento / Physiological potential of soybean seeds submitted to industrial treatment with biostimulant before and after storage

O objetivo deste estudo foi analisar o perfil dos solicitantes dos pedidos de acesso à informação a partir dos dados da plataforma Fala.BR. Como suporte teórico, foi utilizada a literatura sobre o comportamento de busca informacional, além de aspectos da lei de acesso à informação (LAI), transparência e accountability. A coleta de dados foi realizada em maio/2021 e considerou o período de 2012 a 2021. Mais de 260.000 observações, incluindo pessoas físicas e jurídicas, foram examinadas. Os resultados apontaram que o perfil do solicitante – pessoa física, em geral, é caracterizado por ser do sexo masculino, com idade de 30 a 47 anos, com ensino superior completo, servidor público ou acadêmico e que reside na região Sudeste. Quanto ao solicitante - pessoa jurídica, a maioria é brasileira, está localizada na região Sudeste e é de pequeno ou médio porte. Apesar destes achados, é pertinente apontar que o perfil do solicitante – pessoa física vem se alterando nos últimos anos. Houve um crescimento proporcional dos pedidos feitos por pessoas do sexo feminino, com ensino médio e da faixa de idade de 12 a 29 anos. Futuras investigações podem avaliar os motivos do aumento dos pedidos por indivíduos com estas características.