(1) Background: The shade of resin-based materials and ceramic thickness influence the optical color of laminate restorations. The purpose of this study is to evaluate—in vitro—the effect of resin-based cement shade and ceramic thickness on fluorescence of feldspathic laminate veneers; (2) Methods: 180 samples of feldspathic ceramic A2 shade with two different thicknesses (0.5 and 0.8 mm) were obtained. The samples were cemented to composite resin substrates with one of the following materials in different shades (n = 10): resin cement (Variolink Esthetic in Light, Neutral and Warm shades; or RelyX Veneer in B0.5 /white, Translucent and A3 Opaque/yellow opaque shades); flowable composite resin (G-aenial Flo in A2 and A3 shades) or a pre-heated composite resin (Filtek Supreme XTE, A3 body shade). The fluorescence spectra were obtained by means of a spectrofluorometer. Two-way ANOVA, Tukey, and Student’s t-tests were performed (α = 0.05); (3) Results: Fluorescence values were significantly influenced by the resin-based agent tested (p < 0.001), the thickness of ceramic (p < 0.001), and their interaction (p < 0.001). The lowest fluorescence values were achieved by RelyX Veneer resin cement regardless its shade and the ceramic thickness; (4) Conclusions: both the shade of resin-based agent and the feldspathic ceramic thickness influenced the fluorescence of laminate restorations.