Due to rapid urbanization and economic development, the natural environment and ecological processes have been significantly affected by human activities. Especially in ecologically fragile karst areas, the ecosystems are more sensitive to external disturbances and have a hard time recovering, thus studies on the ecosystem services in these areas are significant. In view of this, we took Guizhou (a typical karst province) as the research area, evaluated the ecosystem service value (ESV) according to reclassified land uses and revised equivalent factors, and investigated the determinants of ecosystem services based on geographic detection. It was found that the total ESV showed a prominent increase trend, increasing from 152.55 billion CNY in 2000 to 285.50 billion CNY in 2020. The rise of grain prices due to growing social demands was the main factor in driving the increase of ESV. Spatially, the ESVs of central and western Guizhou were lower with cold spots appearing around human gathering areas, while that of southern and southeastern Guizhou were higher with hot spots that formed in continually distributed woodland. Moreover, the ESV per unit area and its change rate in karst regions were always lower than that in non-karst areas. Precipitation and temperature were the dominant nature factors while cultivation and population density were the main anthropogenic effects driving the evolution of ecosystem services. Therefore, positive human activities as well as rational and efficient land-use should be guided to promote the coordinated and high-quality development of ecology and the economy.

Lake wetlands play a crucial role in mitigating climate change. Human activities and climate change impact the carbon sequestration capacity of lake wetlands. However, this process is intricate. Clarifying the decisive factors that affect carbon sequestration is crucial for preserving, utilizing, and enhancing the carbon sequestration capacity of plateau lake wetlands. Here we analyzed the regulatory role of land use under policy guidance on the carbon sequestration capacity of the plateau lake wetland of Caohai (CHLW), SW China. The results show that: (1) The cumulative carbon sequestration varied significantly from 1990 to 2020, with the highest carbon sequestration of 15.80 × 10⁵ t C in 1995 and the lowest of 3.18 × 10⁵ t C in 2020, mainly originating from endogenous carbon sequestration within the plateau lake wetlands. (2) As of 2020, the carbon stock of CHLW was approximately 2.54 × 10⁸ t C. (3) The carbon sequestration in CHLW experienced a dynamic change process of decrease-increase-decrease over 30 years, mainly influenced by land use changes under policy regulation, with human and natural factors accounting for 91% and 9%, respectively. (4) Under three simulated scenarios (Q1, Q2, and Q3), the ecological priority scenario exhibited positive regulation on the carbon sequestration of CHLW and the entire protected area in 2030 and 2060, with the highest increase in carbon sequestration. This scenario is consistent with the current conservation policy, indicating that the current protection policy for CHLW is scientifically reasonable. This research demonstrates how land use and climate changes impact carbon storage in wetlands, with consideration of policy guidance. It provides references for utilizing and conserving lake wetlands worldwide, ultimately achieving the dual goals of wetland conservation and carbon neutrality.

Black soldier fly have been shown to be one of the optimal alternatives to fishmeal, but there are few reports on the effects of zymolytic black soldier fly larvae (ZBSFL) on the growth and digestion of crustaceans. An 8-week feeding trial was conducted to evaluate the effects of different replacement levels of ZBFLS on growth performance, body composition, and digestive enzyme activity of the mud crab larvae. Four diets were formulated by replacing fishmeal with 0%, 5%, 10% and 15% ZBSFL in the basal diet. Crab larvae were randomly divided into four groups of three replicates each and fed twice daily. The results showed that the SR of crab larvae was higher than that of the no-substitution group when the substitution rate reached 5% (P < 0.05). There was no significant change in SR when the substitution rate was further increased. Weight growth rate and Specific growth rate were similar, both highest at 10% substitution ratio. The crude protein content of whole crab larvae gradually increased as the proportion of FM substituted by ZBSFL increased. The lipid content of whole crab larvae in the 5% substitution ratio group was significantly higher than that in all other groups (P < 0.05). Meanwhile. The activities of amylase, protease and lipase gradually increased. In this experiment, when the percentage of ZBSFL substitution for FM reached 10%, its growth performance was optimal, with higher SR, less negative effects and more balanced indicators in all aspects. When the substitution rate was further increased, it might increase the digestive burden of the crab and negatively affect its growth.

Ecological civilisation has become China’s national strategy to promote harmonious coexistence between man and nature. China is a country that contains vast amounts of wilderness, while facing the threats from reduction and degradation of wilderness areas. As ecological civilisation provides new opportunities for rewilding, this chapter analyses how to incorporate rewilding into the core actions of ecological civilisation, including the National Territory Spatial Planning System (NTSPS), national parks and protected areas, and ecological protection and restoration projects of Mountains-Rivers-Forests-Farmlands-Lakes-Grasslands (MRFFLG). Rewilding and ecological civilisation are both developing concepts, and integrated thinking on these two concepts will promote related research and practice.