Bu çalışma, Allium schoenoprasum bitkisinin etanol ektresinin, insan sağlığını etkileyen bazı antioksidan parametreler ve patojen mikroorganizmalar üzerindeki etkisi araştırılmıştır. Bu kapsamda, A. schoenoprasum etanol ekstresinin antioksidan etkisini belirlemek için DPPH (1,1-difenil-2-pikrilhidrazil) yöntemi kullanılmıştır. Lipid peroksidasyonunu önleme aktivitesi, TBA (Tiyobarbitürik asit) yöntemi ile değerlendirilmiştir. Ayrıca bitki ekstresinin bazı patojenik bakteriler üzerindeki antimikrobiyal etkinliği disk difüzyon yöntemi ile araştırılmıştır. Toplam flavonoid bileşenleri belirlemek içinde kuersetin kullanılmıştır. Bulgularımıza göre, 0.2 mg/ml etanol ekstresinde bulunan toplam flavonoid bileşen miktarı 44.465 ± 0.204 μg/ml ile kuersetine eşdeğer olarak bulunmuştur. Ayrıca bitki ekstresinin lipid peroksidasyonunu önleme aktivitesi, pozitif kontrol olan BHA (Beta hidroksi asit) ile kıyaslandığında A. schoenoprasum etkili olduğu görülmüştür. DPPH radikal söndürme aktivitesi en düşük ve en yüksek konsantrasyon değerleri, % 60,125 - 96,285 iken BHA için bu değerler % 82,178 - 97,357 olduğu bulunmuştur. A. schoenoprasum’un etanol ekstresinin, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans gibi bazı patojen mikroorganizmalara karşı antimikrobiyal aktivite gösterdiği belirlenmiştir.

Active substance production at the nano-level attracts attention in the scientific world due to its wide application area. Different methods are used for the biosynthesis of nanoparticles. Recent studies have focused on non-toxic, environmentally friendly synthesis methods. Nanoparticles obtained by green synthesis using various biological elements such as plants, microorganisms and proteins have taken part in many scientific studies. Plants, which have an important potential in active ingredient production, are highly preferred in nanoparticle production. Scanning Electron Microscope and Energy Dispersive X-Ray Analysis (SEM / SEM-EDX), Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD) and Ultraviolet visible light absorption Spectroscopy (UV-vis) techniques were used for the structural and morphological characterization of Zn nanoparticles obtained by green synthesis using Helichrysum arenarium plant extract and ZnO.The antioxidant capacity of Zn NPs/Ha structures was determined by performing the DPPH test. Antimicrobial effects of zinc nanoparticles on six different pathogens (Bacillus cereus ATCC 10876, Escherichia coli ATCC 25952, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATTC 29213, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATTC 90028) were investigated. As a result of this studies, it has been observed that it has an inhibitory effect against some pathogen microorganisms. It has also been found that its antioxidant content is at a significant level.

Metal nanoparticles have been intensely researched and developed in recent years due to their superior properties. There is a growing interest in economical and environmentally friendly techniques. Cu metal is preferred in research studies due to its cheapness and effects on human health (such as production of blood cells, oxidation and reduction). In recent years, the use of plant extracts in nanoparticle synthesis has become quite popular. In this study, we aimed to investigate the antioxidant capacity and protective properties of Cu nanoparticles obtained by using Diplotaenia turcica plant against damage to pBR322 plasmid DNA. In addition, we investigated the antimicrobial effect of Cu nanoparticles against Staphylococcus aureus ATTC 29213, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25952, Candida albicans ATCC 25952. Diplotaenia turcica plant characterization with Cu metal (Cu NPs/Dt) ultraviolet and visible light absorption spectroscopy (UV-vis), fourier-transformed infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive X-ray (EDX) analysis. When looking at the antioxidant activity analysis, it is understood that it is a powerful antioxidant. It was determined that Cu NPs/Dt have significant antimicrobial activity. Its effect against some pathogens was found to be stronger than the positive control antibiotic. It was determined by the obtained DNA images that there is a potential to prevent breaks that may occur in DNA depending on the concentration.

Green synthesis of copper nanoparticles (Cu NPs) is an economical, environmentally friendly and non-toxic approach that has been the subject of research in health and industry. Therefore, in this study, Cu NPs were synthesized using Pimpinella anisum (P.anisum) seed extract and their pharmacological activities were evaluated. Characterization of Cu NPs was performed by UV-vis, FT-IR and SEM-EDX analyses. Copper metal was reduced by reacting with the seed extract and reached the maximum peak at 385 nm in the UV-vis spectra, confirming the surface plasmon resonance. FT-IR spectroscopy showed the participation of phytochemical components in P. anisum in the synthesis. SEM analysis determined that the size of the biosynthesized nanoparticles is 10-20 nm in diameter and has a spherical structure. Strong signals of copper metal were confirmed by EDX analysis. The therapeutic effect of Cu NPs was evaluated by antioxidant and antibacterial assays. The DPPH radical scavenging activity IC50 inhibition values of Cu NPs were better than the seed extract and exhibited strong antioxidant activity. Antibacterial activity was performed by the disk diffusion method and Cu NPs were more effective against gram-positive bacteria. It had the highest zone diameter (18.0±2.8 mm), especially on Bacillus subtilis bacteria. These results showed that Cu NPs may have a selective effect against drug-resistant bacteria as an alternative agent to pharmaceutical applications. This study showed that P. anisum seed extract-mediated bioconjugation of Cu NPs can be done simply, quickly and cost-effectively. As a result, Cu NPs should be supported by more detailed in vivo studies to create antioxidant and antibacterial agents.

The use of nano-agents in the healthcare field is an important alternative in reducing the increasing pathogen resistance. One of the main purposes of the nano mechanism is that the secondary metabolites produced naturally in plants become more effective through metals. Silver (Ag) nanoparticles were obtained by green synthesis using Veronica beccabunga plant extract and AgNO3 in our study. Scanning electron microscopy was used for SEM, SEM / EDX images of synthesized silver nanoparticles. The interaction of V. beccabunga with Ag was explained by X-ray diffraction (XRD) analysis. The characterization process was performed using an ultraviolet-visible (UV-vis) spectrophotometer and Fourier converted infrared spectroscopy (FT-IR). It was determined that silver nanostructures have an important antioxidant potential as a result of 2,2-difenil-1-pikrilhidrazil (DPPH) analysis. The antimicrobial activity of synthesized metal nanoparticles was investigated, against some pathogens causing disease in humans, by the disk diffusion method. Ag NPs /Vb has been found to have antibacterial effects against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, Pseudomonas aeruginosa ATCC 27853 microorganisms. In our current study, Ag NPs / Vb appears to provide a remarkable effect for its use in medicine, pharmacology.