Industrial production of novel microalgal isolates is key to improving the current portfolio of available strains that are able to grow in large-scale production systems for different biotechnological applications, including carbon mitigation. In this context, Tetraselmis sp. CTP4 was successfully scaled up from an agar plate to 35- and 100-m³ industrial scale tubular photobioreactors (PBR). Growth was performed semi-continuously for 60 days in the autumn-winter season (17^th October – 14^th December). Optimisation of tubular PBR operations showed that improved productivities were obtained at a culture velocity of 0.65–1.35 m s⁻¹ and a pH set-point for CO₂ injection of 8.0. Highest volumetric (0.08 ± 0.01 g L⁻¹ d⁻¹) and areal (20.3 ± 3.2 g m⁻² d⁻¹) biomass productivities were attained in the 100-m³ PBR compared to those of the 35-m³ PBR (0.05 ± 0.02 g L⁻¹ d⁻¹ and 13.5 ± 4.3 g m⁻² d⁻¹, respectively). Lipid contents were similar in both PBRs (9–10% of ash free dry weight). CO₂ sequestration was followed in the 100-m³ PBR, revealing a mean CO₂ mitigation efficiency of 65% and a biomass to carbon ratio of 1.80. Tetraselmis sp. CTP4 is thus a robust candidate for industrial-scale production with promising biomass productivities and photosynthetic efficiencies up to 3.5% of total solar irradiance.