The superfamily Lauxanioidea is a significant dipteran clade including over 2500 known species in three families: Lauxaniidae, Celyphidae and Chamaemyiidae. We sequenced the first five (three complete and two partial) lauxanioid mitochondrial (mt) genomes, and used them to reconstruct the phylogeny of this group. The lauxanioid mt genomes are typical of the Diptera, containing all 37 genes usually present in bilaterian animals. A total of three conserved intergenic sequences have been reported across the Cyclorrhapha. The inferred secondary structure of 22 tRNAs suggested five substitution patterns among the Cyclorrhapha. The control region in the Lauxanioidea has apparently evolved very fast, but four conserved structural elements were detected in all three complete mt genome sequences. Phylogenetic relationships based on the mt genome data were inferred by Maximum Likelihood and Bayesian methods. The traditional relationships between families within the Lauxanioidea, (Chamaemyiidae + (Lauxaniidae + Celyphidae)), were corroborated; however, the higher-level relationships between cyclorrhaphan superfamilies are mostly poorly supported.

Rhopalosole magnispina sp. nov., a new species of R. shaanxiensis Yang & Yang, 1994 species Group of stonefly family Leuctridae is described, illustrated, and compared with related taxa. The female of R. horvati Sivec & Harper, 2008 is associated based on mating pairs collected in the Qinling Mountains of Shaanxi Province. The original female of R. horvati was erroneously associated and considered herein as an unidentified Rhopalosole female of the R. shaanxiensis Group.

The subgenus Homoneura Malloch, 1927 is the most diverse in China, with more than 220 known species. Four new species of Homoneura (Homoneura) henanensis group, that is, Homoneura (Homoneura) apiconcava sp. nov., H. (H.) chongqingensis sp. nov., H. (H.) wuxica sp. nov., and H. (H.) yintiaolingica sp. nov., were described from Yintiaoling Nature Reserve, Chongqing, China. A key to the known species of this species group is presented.

The genus Homoneura comprises over 700 described species in eight known subgenera distributed worldwide and has the highest species richness of Lauxaniidae. Five subgenera and more than 200 species have currently been recorded from China. Despite its high diversity, the monophyly of Homoneura and its subgenera, and the phylogenetic relationships among its subgenera remain to be investigated. One maximum-parsimony tree was generated based on 105 morphological characters scored from 24 species, representing all five subgenera of Homoneura recorded from China. The results did not support the monophyly of the genus Homoneura and subgenus Homoneura. The subgenus Chaetohomoneura is a sister to subgenus Neohomoneura. The monophyly of the subgenera Euhomoneura and Neohomoneura is supported. Much of the current classification of the genus Homoneura needs a revision before taxonomy can reflect natural groupings.

House flies (Muscidae) comprise the most species-rich family of the muscoid grade with over 5000 described species worldwide, and they are abundant in various terrestrial and aquatic ecosystems. The high number of species, varied appearances, complex feeding habits, and wide distributions have hindered researchers from understanding their phylogeny and evolutionary history. Here, we newly sequenced fifteen mitochondrial genomes and reconstructed the phylogenetic relationships and divergence time among eight subfamilies of Muscidae (Diptera). The best phylogenetic tree, which was inferred by IQ-Tree, recovered the monophyly for seven out of eight subfamilies (except for Mydaeinae). Based on phylogenetic analyses and morphological characteristics, we prefer the subfamily status of Azeliinae and Reinwardtiinae, and separate Stomoxyinae from Muscinae. Genus Helina Robineau-Desvoidy, 1830 was synonymized with Phaonia Robineau-Desvoidy, 1830. The divergence time estimation indicated Muscidae originated at 51.59 Ma (early Eocene). Most subfamilies had originated around 41 Ma. We provided a mtgenomic viewpoint on the phylogenetic relationships and divergence time estimation of Muscidae.

A new species of the genus Suwallia Ricker, 1943, S. rostrata sp. nov., from Xizang of southwestern China is described, illustrated and compared with similar species. Alloperla kurentzovi Zhiltzova & Zapekina-Dulkeit, 1977, A. kurilensis Zhiltzova, 1978, A. teleckojensis Šámal, 1939 are reported for the first time from China. The diagnostic features of these species are illustrated with color images.