This study was conducted to reveal the prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products including powdered infant formula, raw milk, pasteurized milk, ultra-high-temperature milk, and cheese. Five hundred samples collected from 5 provinces in China were analyzed in overall experiments. Multilocus sequence typing, distribution of toxin genes, and antibiotic susceptibility of the isolates were analyzed. Fifty-four B. cereus strains were detected; of these, 13 isolates (26%) were from raw milk, 12 isolates (12%) from pasteurized milk, 10 isolates (10%) from cheese, 12 isolates (8%) from ultra-high-temperature milk, and 7 isolates (7%) from powdered infant formula. These isolates were divided into 24 sequence types (ST); among them, ST24, ST26, ST82, ST142, ST377, ST857, and ST1046 were the main dominant ST. The results of detection of toxin genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, bceT, hlyII, and cesB) showed that 94.4% isolates carried nheABC genes, whereas only 11.1% of the isolates contained the hblACD gene cluster. In addition, detection rates of cytK, bceT, entFM, hlyII, and cesB genes were 75.9, 77.8, 85.2, 53.7, and 11.1%, respectively. The antibiotic susceptibility test indicated that most of B. cereus isolates were resistant to ampicillin, penicillin, cefepime, cephalothin, and oxacillin, and were susceptible to gentamicin, chloramphenicol, imipenem, tetracycline, ciprofloxacin, trimethoprim-sulfamethoxazole, erythromycin, kanamycin, and cefotetan. Therefore, this study revealed the prevalence and characteristics of B. cereus isolated from dairy products in China, indicating the potential risk and contributing to the effective prevention and control of this pathogen.

The genus Homoneura comprises over 700 described species in eight known subgenera distributed worldwide and has the highest species richness of Lauxaniidae. Five subgenera and more than 200 species have currently been recorded from China. Despite its high diversity, the monophyly of Homoneura and its subgenera, and the phylogenetic relationships among its subgenera remain to be investigated. One maximum-parsimony tree was generated based on 105 morphological characters scored from 24 species, representing all five subgenera of Homoneura recorded from China. The results did not support the monophyly of the genus Homoneura and subgenus Homoneura. The subgenus Chaetohomoneura is a sister to subgenus Neohomoneura. The monophyly of the subgenera Euhomoneura and Neohomoneura is supported. Much of the current classification of the genus Homoneura needs a revision before taxonomy can reflect natural groupings.

This study performed mitochondrial genome sequencing on Minettia omei, which belongs to Lauxaniinae, a subfamily of Lauxaniidae. The complete mitochondrial genome spans 16,201 bp and comprises 37 genes, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region. Base composition: A 38.81%, T 38.81%, C 12.93%, G 9.44% , A + T bias 77.62%. COI uses start codon CAA; COI, COII, NAD2, NAD1, NAD4 have incomplete T + tRNA stop codons. Phylogenetic analysis elucidates the species' evolutionary relationships, establishing a critical genetic foundation for future studies on Lauxaniidae.

In order to reveal the fermentation microflora and fermentation metabolites of traditional mung bean sour liquid (MBSL) in Luoyang area, China, this experiment was sampled from four administrative districts of Luoyang, and volatile metabolites and non-targeted metabolites were detected and analyzed by HS-SPME-GC-MS and LC-MS, and bacterial and fungal sequencing were analyzed by Illumina MiSeq high-throughput sequencing technology. And the correlation between microorganisms and metabolites was conducted. The results showed that 42 volatiles were detected in four MBSL samples named Jianxi (JX), Liujia (Liu), LiJia (LJ), Majia (MJ), with 11 identical volatile flavor subtances, and the highest content of esters was found in JX, Liu, and LJ, and the highest content of acids was found in MJ. A total of 1703 non-targeted metabolites were identified, and there were more types of amino acids, carbohydrates, fatty acids and their complexes, flavonoids, carbonyl compounds, and organic acids, accounting for 40.93%. High-throughput sequencing results showed that there were nine bacterial and fungal genera with an average abundance of more than 1%, and the dominant genera mainly belonged to lactic acid bacteria and yeast. The composition of dominant genera was different in different workshop samples, and the abundance of fungal genera differed greatly. Among the volatile substances, Methyleugenol, a volatile component, was related to more bacteria, and ketones and hydrocarbons may be more closely associated with bacteria. Acetic acid and Oxalic acid may be more closely related to fungi, while some esters were more closely related to both fungal and bacterial genera. For non-target metabolites, amino acid and alcohol metabolites may be more influenced by bacteria, and organic acids and flavonoids may be more influenced by fungi.

The genus Sapromyza Fallén (Diptera: Lauxaniidae) represents the second most species-rich genus within the family, exhibiting a broad distribution across major zoogeographical regions (Papp et al. ...