Purpose: This study aims to examine the effects of red 649 nm 4 J/cm²and blue 403 nm 8 J/cm²diode laser treatment for post-extraction wounded healing in rats through histopathological and immunohistochemical analysis.Methods: Samples of 54 Wistar rats were divided into six groups: C- control group without treatment; C + wounded group without treatment; TB wound group with Povidone-iodine treatment; TD wounded group with doxycycline treatment; TLB wounded group with 403 nm diode laser treatment; and TLR wounded group with 649 nm diode laser treatment. Mandibular samples were observed for the number of lymphocytes and fibroblasts cells, new blood vessels formation, Interleukin 1β, and Collagen 1α expression level.Results: Based on the histopathological test results, red laser diode treatment significantly increased the number of lymphocyte, fibroblast cells and the formation of new blood vessels. Meanwhile, immunohistochemical tests showed an increase in the expression of the Colagen-1α protein which plays a role in the formation of collagen for new tissues formation after damage, as well as a decrease in Interleukin-1β expression level. Blue laser is also able to show a positive effect on wound healing even though its penetration level into the tissue is lower compared to red laser.Conclusion: The red diode laser 649 nm has been shown to accelerate the process of proliferation in wound healing post molar extraction based on histopathological and immunohistochemical test results.

Imprinted zeolite modified carbon paste (carbon paste-IZ) electrode had been developed as a sensor to analyze blood glucose content by potentiometry. The used zeolite was Lynde Type A (LTA) that synthesized with a mole ratio of Na2O, Al2O3, SiO2 and H2O of 4:1:1.8:270, respectively while non-imprinted zeolite was prepared with a mole ratio of glucose/Si of 0.0306. Glucose was then extracted from the zeolite framework using hot water (80 °C) to produce imprinted zeolite (IZ). The carbon paste-IZ electrode prepared from activated carbon, paraffin pastilles, and IZ with a mass ratio of 5:4:1 showed the best performance. The modified electrode demonstrated the measurement range of 10–4-10–2 M, the Nernst factor of 29.55 mV/decade, the response time less than 120 s, and the detection limit of 5.62 × 10–5 M. Ascorbic acid, uric acid, urea and creatinine did not interfere on the glucose analysis by potentiometry. Comparison test with spectrophotometry showed an accuracy of (90.7 ± 1.4)% (n = 5), while the application of the electrode to analyze five spiked serum samples showed recovery of (92.2 ± 1.3)% (n = 5). The electrode was stable for up to 9 weeks (168 times usage). Based on its performance, the developed electrode can be applied to analyze glucose in human serum sample and recommended for used in the medical field.

Carbon paste electrode-modified imprinted zeolite X has been developed as a potentiometry and voltammetry sensor to monitor cholesterol levels in the body. This is crucial to detect early health risks caused by high cholesterol levels. The modified electrode was fabricated with a mass ratio of activated carbon, paraffin, and imprinted zeolite X of 12:7:1. Potentiometric measurement produced a linear dynamic range of 10−6–10−3 M, Nernst factor of 27.12 mV/decade, a detection limit of 1.12 × 10−6 M, precision of 99.7% (n = 3), and accuracy of 99.8% (n = 5). Using the electrode for up to 56 measurements over 6 weeks did not significantly decrease its performance. The presence of glucose did not interfere with cholesterol analysis by potentiometry. The modified electrode was applied to analyze cholesterol voltammetrically at the optimum deposition potential of 0.4 V, deposition time of 60 s, and a scan rate of 100 mV/s. Voltammetric analysis of cholesterol resulted in a detection limit of 7.2 mg/L (1.86 µM), precision of 96–99%, accuracy of 74–114%, sensitivity of 7.1 nA.L/mg/cm2, and recovery of 87.2% (n = 3). The glucose and urea in various concentrations cause less than 5% current deviations.

A new compound (E)-3-[3-(4-morpholinophenyl)acryloyl]-2H-chromen-2-one, a coumarin based chalcone derivative, has been successfully synthesized employing a molecular hybridization method through the reaction between 3-acetylcoumarin and 4-morpholinobenzaldehyde using a Claisen–Schmidt reaction using pTSA as a catalyst. The structure of the title compound was established using spectroscopic data FTIR, HRESI-MS, ¹H- and ¹³C-NMR. The anticancer activity against breast cancer cells line T47D and cervix cancer cells line HeLa was determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.

The development of sensors based on imprinted zeolite X to detect blood glucose through potentiometry was performed. In this study, the sensor was made of a mixture of carbon paste and imprinted zeolite X. Zeolite X was synthesized using a sol–gel-hydrothermal method at a temperature of 100 °C with basic materials of NaAlO₂, NaOH, TEOS, and distilled water. The characterization results of XRD showed the presence of specific peaks, which were confirmed with standard zeolite X. Imprinted zeolite X exhibited a 20 times greater adsorption capacity size, and an adsorption efficiency 3 times greater than that of zeolite X. This is thought to be due to the presence of a molecular template within it. The IZ–carbon paste electrode showed optimum performance due to a mass ratio of carbon, paraffin, and imprinted zeolite X of 12:7:1. The electrode performance was expressed by the Nernst factor value of 30 mV/decade, the measuring range of 10⁻⁴–10⁻² M, the upper detection limit of 1.38 × 10⁻² M, and the lower detection limit of 1.28 × 10⁻⁴ M, so this electrode can be used for glucose analysis with a normal concentration (70–110 mg/dL or equivalent to 3.8 × 10⁻³–6.1 × 10⁻³ M), as well as the glucose concentration of people with diabetes mellitus (>200 mg/dL or about 10⁻² M). This electrode showed precision values of 97.14–99.02%, accuracy values of 98.65–99.39%, and electrode response times of 10–13 s. The electrodes showed high stability for more than 5 weeks with 141 uses. The electrodes also showed high selectivity for glucose in the matrix of uric acid, urea, NaCl, and KCl. Therefore, its use as an alternative electrode for routine glucose analysis in the medical field is recommended.