A simplified, fast, and effective production method has been developed for the synthesis of manganese ferrite (MnFe₂O₄) magnetic nanoparticles (MNPs). In addition to the wide applicability of MnFe₂O₄ MNPs, this work also reports their application in DNA isolation for the first time. An ultrasonic-cavitation-assisted combustion method was applied in the synthesis of MnFe₂O₄ MNPs at different furnace temperatures (573 K, 623 K, 673 K, and 773 K) to optimize the particles’ properties. It was shown that MnFe₂O₄ nanoparticles synthesized at 573 K consist of a spinel phase only with adequate size and zeta potential distributions and superparamagnetic properties. It was also demonstrated that superparamagnetic manganese ferrite nanoparticles bind DNA in buffer with a high NaCl concentration (2.5 M), and the DNA desorbs from the MNPs by decreasing the NaCl concentration of the elution buffer. This resulted in a DNA yield comparable to that of commercial DNA extraction products. Both the DNA concentration measurements and electrophoresis confirmed that a high amount of isolated bacterial plasmid DNA (pDNA) with adequate purity can be extracted with MnFe₂O₄ (573 K) nanoparticles by applying the DNA extraction method proposed in this article.

Sonochemistry in combination with a combustion procedure was used to prepare superparamagnetic iron oxide nanoparticles. Two different iron precursors, ferrocene and iron(II)-acetate were dispersed in polyethylene-glycol (PEG) with different molecular weight (200–1000) by using a high efficiency ultrasonic technique. The sonochemical treatment initiated the precipitation of iron-hydroxide particles, then the PEG based colloids were burnt to create the iron oxide nanoparticles. Three phases were observed in the samples, magnetite (Fe₃O₄), hematite (α-Fe₂O₃), and maghemite (γ-Fe₂O₃) and their ratio can be modified by changing the iron precursor and the type of PEG. Elektrokinetic (Zeta) potential and the particle size of the synthesized iron oxide nanoparticle samples were measured and the one (A1) based on iron(II)-acetate and PEG200 have been selected to test in DNA purification, because it has negative and small size distribution. The synthesized superparamagnetic nanoparticles have been used successfully in biological isolation (DNA, deoxyribonucleic acid purification), and thus, the developed synthetic procedure is more than suitable for such applications.

The presence of industrially produced chemicals in water is often not monitored, while their passive transport and accumulation can cause serious damage in living cells. Molecular dynamics simulations are an effective way to understand the mechanism of the action of these pollutants. In this paper, the passive membrane transport of 1,4-dioxane, phenol, oxane and morpholine was investigated and analyzed thoroughly from structural and energetic points of view. Free energy profiles for pollutant and water penetration into the bilayer were obtained from well-tempered metadynamics (WT-MD) simulations and a mass density-based approach. It was found that all four investigated compounds can penetrate biological membranes and affect the free energy profile of water penetration. Out of the investigated species, oxane has the thermodynamically most preferred position in the bilayer center, leading to a lower free energy barrier of water molecules by 3 kJ mol⁻¹, resulting in 5 times more water molecules in the bilayer center. The concentration dependence of free energy was tested at two different phenol concentrations using WT-MD, and it was found that the higher phenol concentration lowers the main barrier by 3 kJ mol⁻¹. Density-based free energy calculations were found to reproduce the results of WT-MD within the limits of chemical accuracy.

In this article, we describe the antimicrobial properties of a new composite based on anodic aluminium oxide (AAO) membranes containing propyl-copper-phosphonate units arranged at a predetermined density inside the AAO channels. The samples were prepared with four concentrations of copper ions and tested as antimicrobial drug on four different strains of Escherichia coli (K12, R2, R3 and R4). For comparison, the same strains were tested with three types of antibiotics using the minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. Moreover, DNA was isolated from the analysed bacteria which was additionally digested with formamidopyrimidine-DNA glycosylase (Fpg) protein from the group of repair glycosases. These enzymes are markers of modified oxidised bases in nucleic acids produced during oxidative stress in cells. Preliminary cellular studies, MIC and MBC tests and digestion with Fpg protein after modification of bacterial DNA suggest that these compounds may have greater potential as antibacterial agents than antibiotics such as ciprofloxacin, bleomycin and cloxacillin. The described composites are highly specific for the analysed model Escherichia coli strains and may be used in the future as new substitutes for commonly used antibiotics in clinical and nosocomial infections in the progressing pandemic era. The results show much stronger antibacterial properties of the functionalised membranes on the action of bacterial membranes in comparison to the antibiotics in the Fpg digestion experiment. This is most likely due to the strong induction of oxidative stress in the cell through the breakdown of the analysed bacterial DNA. We have also observed that the intermolecular distances between the functional units play an important role for the antimicrobial properties of the used material. Hence, we utilised the idea of the 2D solvent to tailor them.

Polyurethanes (PUs) are a special class of polymeric materials that differ significantly from most other types of plastic in many aspects. They can be utilized in a wide range of products, including paints, coatings, elastomers, insulators, elastic fibers, and foams. PU foams are especially important as part of various convenience products. PU products often end up in landfills when they are no longer useful and can release toxic compounds when damaged by humans or microbes. Therefore, the ecotoxicological assessment of PU foams is essential. In this paper, five PU foam samples were prepared with different NCO indices (NCO-0.8, 0.9, 1.0, 1.1, and 1.2) and together with the Control sample (a previously tested non-toxic foam sample) were applied to develop toxicity tests procedure, while intentionally prepared Toxic foam has been used to verify the accuracy of the developed testing procedure. Two test organisms were successfully applied, Sinapis alba (white mustard) seeds and Escherichia coli (non-pathogenic) bacterial model organisms, and toxicity tests were adapted for the examination of PU-derived substances. Regarding Sinapis alba test, the highest NCO index (NCO-1.2) significantly reduced root length by 9.8 % compared to the Control sample. In the bacterial test, it was observed that the samples containing NCO-1.1 and NCO-1.2 had lower colony numbers (5.0 × 10⁸ and 4.9 × 10⁸ CFU/mL respectively) in comparison to the Control plate (9.6 × 10⁸ CFU/mL). All in all, two toxicity tests were successfully adapted for PU foams, and both are applicable in their ecotoxicological assessment.

Activated carbon (AC) and biochar (BC) are porous materials with large surface areas and widely used in environmental and industrial applications. In this study, different types of AC and BC samples were produced from Sargassum sp. by a chemical activation and pyrolysis process and compared to commercial activated carbon samples. All samples were characterized using various techniques to understand their structure and functionalities. The metal content of the samples was characterized by using an inductively coupled optical emission spectrometer (ICP-OES). A toxicity test was applied to investigate the effect of AC/BC on organisms, where Sinapis alba seed and Escherichia coli bacteria-based toxicity tests were used. The results revealed that the samples did not negatively affect these two organisms. Thus, it is safe to use them in various applications. Therefore, the samples were tested as fillers in polyurethane composites and, thus, polyurethane-AC/BC samples were prepared. The amounts of AC/BC mixed into the polyurethane formulation were 1%, 2%, and 3%. Mechanical and acoustic properties of these composites were analyzed, showing that by adding the AC/BC to the system an increase in the compression strength for all the samples was achieved. A similar effect of the AC/BC was noticed in the acoustic measurements, where adding AC/BC enhanced the sound adsorption coefficient (α) for all composite materials.