Background: To limit the impact of the downy mildew disease of grapevine and reduce the need to recur to chemical treatments, an effective strategy might be recovering adaptive resistance traits in both cultivated and wildV. viniferagermplasm.Considering that stilbenes represent the most important class of phytoalexins in theVitaceae, the constitutive expression and transcriptional activation of all the functional members of the stilbene synthase gene family were analysed in a group of nine grapevine genotypes following artificial infection with the oomycetePlasmopara viticola, the causal agent of the disease. In addition, in the same genotypes we analyzed the expression of genes encoding for two transcription factors involved in the transcriptional regulation of the stilbene synthase genes, namelyVvMYB14andVvMYB15, and of genes encoding for chalcone synthases.Results: Downy mildew incidence and severity ranged from nihil to high in the grapevine genotypes considered, being low to moderate in a subgroup ofV. viniferagenotypes. The constitutive expression of the stilbene synthase genes as well as the extent of their transcriptional activation followingP. viticolainoculation appeared to be inversely related to the proneness to develop disease symptoms upon infection. In a specular manner, followingP. viticolainoculation all the chalcone synthase genes were up-regulated in the susceptible grapevine genotypes and down-regulated in the resistant ones. The infection brought byP. viticolaappeared to elicit a co-ordinated and sequential transcriptional activation of distinct stilbene synthase genes subsets, each of which may be regulated by a distinct and specific MYB transcription factor.Conclusions: The present results suggest that the induction of stilbene biosynthesis may contribute to the basal immunity against the downy mildew of grapevine, thus representing an adaptive resistance trait to recover, in both cultivated and wildV. viniferagermplasm. During the early stages ofP. viticolainfection, an antagonistic interaction between flavonol and stilbene biosynthesis might occur, whose outcome might determine the subsequent extent of disease symptoms. Further studies are needed to decipher the possible regulatory mechanisms involved in the antagonistic crosstalk between these two metabolic pathways in resistant and susceptible genotypes in response toP. viticola.

Protein stability in bottled white wine is an essential organoleptic property considered by consumers. In this paper, the effectiveness of an early enzymatic treatment was investigated by adding a food-grade microbial protease at two different stages of winemaking: (i) at cold settling, for a short-term and low temperature (10 °C) action prior to alcoholic fermentation (AF); (ii) at yeast inoculum, for a long-lasting and medium temperature (18 °C) action during AF. The results reveal that protease sufficiently preserved its catalytic activity at both operational conditions: 10 °C (during cold settling) and 18 °C (during AF). Furthermore, protease addition (dosage 50–150 μL/L) raised the alcoholic fermentation rate. The treatment at yeast inoculum (dosage 50 μL/L) had a remarkable effect in preventing haze formation, as revealed by its impact on protein instability and haze-active proteins. This minimally invasive, time and resource-saving enzymatic treatment, integrated into the winemaking process, could produce stable white wine without affecting color quality and phenol content.

The European hazelnut is a temperate nut crop, often managed as a multi-stemmed shrub due to its natural aptitude to produce several suckers at the base of the stump, depending on variety. Traditional hazelnut-growing regions such as Italy, Turkey, and Spain typically adopt this architecture, while other hazelnut-producing countries such as the USA, France, and Chile increasingly use single-trunk systems to facilitate orchard management. Multi-stemmed plants allow gradual renewal through sucker selection but may lead to excessively dense canopies, reducing the effectiveness of pest and disease control, increasing biennial bearing, and lowering nut yields in vigorous cultivars. In order to drive the ongoing enlargement of hazelnut cultivation, attempts in designing high-density (HD), and more occasionally super-high-density (SHD), hazelnut orchards are on-going, although these are poorly explored in terms of suitable plant training systems, such that, sometimes, multi-stemmed plant shapes are used; otherwise, single-trunk solutions are adopted. In order to explore new hazelnut planting and training solutions focused on sustainable intensification, a trial was established in 2019 in central Italy to evaluate the eligibility of three training systems (treatment A: regular four-stemmed shrub; treatment B: single-trunk sapling; treatment C: traditional multi-stemmed shrub), applied on unpruned three-year-old plants of the hazelnut cultivar Nocchione, planted in the HD approach (740 plant ha⁻¹). Over five growing seasons (2019–2023), measurements included pruned wood removed, yield, vigor, yield efficiency, nut and kernel traits, and incidence of the main commercial defects. In general, treatment A outperformed other plant-shaping systems, maintaining high yield levels particularly in the two last growing seasons, and showing a mean kernel/nut ratio of 37.7 and a low incidence of defects. Treatment B achieved the highest yield efficiency in 2023 but had lower overall yields. Treatment A demonstrated the most balanced performance, combining high nut quality and stable production, making it the most promising plant training system for HD hazelnut orchards with planting densities above 700 plants per hectare. Future research will assess the long-term adaptability of this plant training system under varying environmental and management conditions.

Most historical sources that describe the presence of Romanesco variety in vine-growing areas of Lazio Region (Italy) highlighted the variability of morphological traits within the variety. This partly justifies the presence of different synonyms, true or presumed, reported by many authors for this grape variety. With the aim of analysing this variability, eight accessions related to the variety, collected in Lazio Region and grown in the DAFNE grape germplasm collection, have been characterized over five productive seasons. The ampelographic description was carried out using 50 OIV morphological descriptors and ampelometric analyses were carried out on mature leaves by SuperAmpelo software. The DNA of the different accessions, extracted from young leaves, was analyzed using 14 microsatellite loci. Furthermore, at harvest, the grapes of each accession were sampled to assess main compositive characteristics. Results showed differences among accessions on some ampelographic descriptors of the mature leaf, of the bunch, and on phenological stages. Microsatellite profiles allowed for classification of the accessions into three distinct groups. Qualitative analysis of the berry skin showed differences among accessions in the content of the main classes of phenolic compounds.

The wide ampelographic treasure of Italian wine grape varieties is driving research towards suitable approaches for the varietal authenticity control of wine. In this paper, Aglianico, Negroamaro, Primitivo and Uva di Troia red wines, which were produced experimentally by single-grape winemaking from non-aromatic grapes native to southern Italy, were analyzed with respect to berry markers, namely anthocyanins, hydroxycinnamic acids (HPLC-DAD), shikimic acid (HPLC-UV) and glycosidic aroma precursors (GC-MS). The study confirms that, just as for the berries, useful varietal authenticity markers for red wine, even after aging, turn out to be hydroxycinnamic acids, relative amounts of acylated forms of anthocyanins, and shikimic acid, together with some grape glycosidic precursors from terpenes and C₁₃− norisoprenoids. Principal Component Analysis was used as a valuable tool to highlight the results.