Soil organic carbon (SOC) is related to the sustainability of the agricultural systems affecting soil properties linked to crop yield. SOC affects the amount and availability of soil nutrients, contributing elements as N, usually deficient. In addition, SOC modify the acidity and the alkalinity towards values near the neutrality, and then it increases the solubility to several nutrients. SOC associated to the soil organic matter provides colloids having high cation exchange capacity. Its effect on the physical properties of the soil is reflected in the soil structure and pore size distribution. SOC concentration depends on the local environmental conditions, but it is also affected strongly by the soil management practices. There are managements that decrease SOC and viceverse other practices that favor the accumulation. The present review deals with the influence of SOC on the chemical, physical, biological properties of the soil and how it is affected by soil management. We also describes methodologies to study C0(2) fluxes from soil to atmosphere

The success of using active restoration in Mediterranean-type climate zones mostly depends on an appropriate matching of plant species and specific management prescriptions upon establishment. In this study, we assessed the early growth and short-term physiological acclimation of seven common species found in the sclerophyllous forests in central Chile to water restriction and shading. We established a nursery experiment that included three treatments (T0: sun-exposed and water-restricted, T1: sun-exposed and fully irrigated, and T2: shaded and fully irrigated) and seven tree species differing in their shade and drought tolerance (Quillaja saponaria Molina, Aristotelia chilensis (Mol.) Stuntz, Peumus boldus Molina, Lithraea caustica (Mol.) Hook. and Arn, Luma apiculata (DC.) Burret, Colliguaja odorifera Molina, and Escallonia pulverulenta (Ruiz and Prav.) Pers). We measured the increment in seedling height and different leaf morpho-physiological traits during two months in the dry season. Based on the measured traits, none of the species took advantage of the higher water availability in T1 relative to T0, but most of the species responded to the shade in T2, regardless of their shade or drought tolerance. Height increments due to shade varied from 0% in P. boldus to 203% in L. apiculata. Overall, all the species responded similarly to the treatments in specific leaf area, chlorophyll content index, photosynthetic rate, stomatal conductance, and intrinsic water use efficiency. This suggests that the species exhibited similar acclimation patterns of these parameters to shade and drought, even regarding the variation in midday xylem water potential found in the water-restricted treatment T0 (from −1.5 MPa in P. boldus to −3.1 MPa in E. pulverulenta). In this study, shading had a higher positive effect on the seedling performance of sclerophyllous species than watering, which at operational level highlights the need for investing in tree shelters when using these species in restoration programs.

Natural regeneration of recalcitrant-seeded tree species is strongly limited in Mediterranean-type climate zones due to increasing droughts imposed by climate change. Direct seeding can be a low-cost alternative to seedling establishment, but there is still limited information for some species. This study aimed to assess the effects of the seed source and forest cover on the germination and survival of the endemic Cryptocarya alba Mol. established through direct seeding. Three habitat types differing in forest cover were identified within the natural park Reserva Natural Altos de Cantillana, Metropolitan Region, Chile. The forest cover corresponded to open (canopy density 75%). All forest cover had C. alba as one of the dominant species. At each habitat type, 38 families from four seed sources (Cuesta La Dormida (CD), Antumapu (AN), Cantillana (CA, local seed source) and Cayumanque (CY)) were directly seeded. Germination (Germin) and survival (Surv) were evaluated weekly during one growing season. There were significant differences between seed sources in Germin and Surv, with means values varying from 7.8% to 37% for Germin and 0% to 20% for Surv. The local seed source CA had the highest values in both traits. A significant variation was also observed between families within seed sources only for Germin. The dense forest cover had the highest Germin (22%) and Surv (55%) results compared to the other forest cover types, which was partially associated with differences in soil moisture, temperature, and bulk density. Due to the most frequent droughts in these Mediterranean-type climate zones, the use of local seeds on dense forest cover is recommended for the direct seeding of the species in the initial recruitment.

As a consequence of the megadrought in Central Chile, it is expected that most of the distribution of woody species will be narrowed in the northern limits because of restrictions imposed by soil matric potential on seed germination. In this study, we analyzed the effect of the soil matric potential on seed germination and initial recruitment of the sclerophyllous species Prosopis chilensis, Quillaja saponaria and Cryptocarya alba from contrasting geographic origins (i.e., seed sources). We evaluated the germination capacity (%) under different matric potentials (i.e., 0, −6, −33, −750 and −1250 kPa) for 100 days. Soil matric potential of −1250 kPa negatively affected the germination capacity of the three species. P. chilensis seeds stopped germinating under soil matric potential close to −1200 kPa, whereas in Q. saponaria and C. alba the complete inhibition of germination was under −1000 kPa. Seed sources also differed in their germination capacity by soil matric potential: northern seed sources of P. chilensis germinated with the lowest soil matric potential. There was no clear trend in Q. saponaria and C. alba, but in general, southern seed sources performed better than the northern ones. The results showed that Ѱ_m in the soil played an important role in the germinative capacity against different seed source origins, but not in soils with a north–south gradient.

We used chlorophyll fluorescence techniques to investigate seasonal variations in photosystem II (PSII) quantum yield in five-year-old saplings of the sclerophyllous Peumus boldus Molina (evergreen) and Colliguaja odorifera Molina (semideciduous) planted in a semiarid site with a Mediterranean-type climate. Chlorophyll fluorescence rise kinetics (OJIP) were monitored monthly for one year (September 2024 to September 2025). With this information, we estimated the relative deviation of the performance index (PI_ABS) of each species from the average PI_ABS in each season (denoted as ∆PI_ABS). P. boldus was associated with destruction of PSII reaction centers and incapacity for electron transport, i.e., higher values of parameters ABS/RC (effective antenna size of an active reaction center) and F₀ (minimal fluorescence), whereas C. odorifera was associated with higher photosynthetic performance i.e., higher values of PI_ABS, PI_TOT (total performance index), F_V/F₀ (ratio between variable and minimal fluorescence), and F_V/F_M (maximum quantum yield of primary PSII photochemistry). P_IABS exhibited a 52 and 38% reduction (i.e., −∆PI_ABS) during spring and winter in P. boldus, but an increase (i.e., +∆PI_ABS) of 52 and 37% in the same seasons for C. odorifera. P. boldus was considerably more depressed during the winter–spring season than the summer months. This suggests that PSII function in P. boldus is more sensitive to low temperatures in winter and spring than the lack of water and high temperatures during summer.

Climate change is threatening the restoration efforts in Mediterranean ecosystems, and there is still little knowledge about the responses of some sclerophyllous species to plant management techniques. This study assessed the effects of the planting date, use of mulch, and controlling light levels on the early survival and growth of sclerophyllous species established in island plots. A factorial design was installed in the Metropolitan Region of central Chile, with a treatment combination that included three planting dates (January: midsummer, April: autumn, and June: winter), three light levels (full sun exposed (T0), shaded at 35% (T35), and shaded at 70% (T70)), and two mulch levels (no mulch versus mulch application). Additionally, we tested the species effects within the island plot. We measured survival, as well as plant diameter and height increments, 1 year after establishment. Each island plot contained three seedlings of Acacia caven Mol., two of Quillaja saponaria Mol., two of Maytenus boaria Mol., one of Schinus polygamus (Cav.) Cabrera, and one of Cryptocarya alba (Mol.) Losser. We found a significant main effect for all the factors assessed. Despite the high mortality in the trial, survival was increased around fourfold by planting in winter, using mulch, or using either of the shading levels. At the species level, average survival ranged from 7% for C. alba to 27% for S. polygamous. To increase plant survival in the restoration of this ecosystem, it is necessary to intensify the management techniques and the use of these types of eco-technologies; if not, the restoration may fail.