Oral papilloma lesions may appear as a result of HPV infection, or not, and only special molecular methods could differentiate them. Low-risk and high-risk HPV types could induce oral HPV papillomatosis with different natural evolution, clearance and persistence mechanisms. The pathogenic mechanisms are based on the crosstalk between the oral epithelial and immune cells and this very efficient virus. HPV acts as a direct inducer in the process of transforming a benign lesion into a malignant one, the cancerization process being also debated in this paper. According to the degree of malignity, three types of papillomatous lesions can be described in the oral cavity: benign lesions, potential malign disorders and malignant lesions. The precise molecular diagnostic is important to identify the presence of various virus types and also the virus products responsible for its oncogenicity. An accurate diagnostic of oral papilloma can be established through a good knowledge of etiological and epidemiological factors, clinical examination and laboratory tests. This review intends to update the pathogenic mechanisms driving the macroscopic and histological features of oral papillomatosis having HPV infection as the main etiological factor, focusing on its interreference in the local immunity. In the absence of an accurate molecular diagnostic and knowledge of local immunological conditions, the therapeutic strategy could be difficult to decide.

Background. Orthodontic therapy is a complex process involving a series of specialists from the dental field: the oral-maxillo-facial surgeon, the periodontologist, sometimes even the prosthetist, implantologist or general doctors. The injuries of the oral mucosa induced by orthodontic therapy include gingival overrgrowths, traumatic lesions of the oral mucosa, different degrees of periodontal damage manifested by gingival retraction, alveolar bone resorption. Materials and methods. From a total of 327 subjects who presented at the Dental Clinic in Craiova, 74 subjects were selected who presented with gingival overgrowth associated with fixed orthodontic therapy. Subjects ranged in age from 14 to 56 years and experienced bleeding and gingival discomfort as well as alterations in physiognomic function. None of the patients included in the study have systemic diseases and are not under medical treatment. The clinical and statistical study took place between May 2022 and December 2023. Each patient was given a personal record containing personal data as well as oral and systemic health status. The examination of the periodontal status aimed at the evaluation of the following indices: assessment of oral hygiene using the OHI-S index and the O’Leary plaque index, assessment of superficial periodontal status using the Löe/Silness gingival inflammation index, periodontometry was performed in order to determine the depth of periodontal pockets, the level of gingival insertion, and the McGaw gingival overgrowth index. OHI-S index comprises two elements: the Debris Index and the Calculus Index. The purpose of our study is to present the incidence of cases of gingival overgrowth induced by fixed orthodontic therapy and to highlight how certain irritating factors can exacerbate the symptoms of gingival overgrowth of orthodontic etiology. Results. The majority of patients are female, aged between 30 and 55 years old. Most clinically examined patients have presented with Grade II gingival hyperplasia. Factors that have exacerbated the symptoms of orthodontically induced gingival overgrowth include: incorrectly adapted prosthetic restorations, unpolished massive coronal fillings, root remnants, bacterial plaque, and tartar.Clinical examination of the oral cavity revealed the presence of gingival inflammation (localized or generalized), simple or complicated, treated and untreated odontal lesions, and coronal fillings made of light-curing composite material of significant size, being unfinished and unpolished, sometimes with sharp edges directly injuring the adjacent gingival mucosa, marginally incorrectly adapted prosthetic works. In the case of child and adolescent patients, significant amounts of bacterial plaque and tartar deposits were observed. In most of the cases examined, it was observed that the gingival overgrowth had a firm consistency, pinkish-reddish colour and gingival bleeding was evident during probing. Conclusion. Gingival overgrowth caused by orthodontics induces a number of important periodontal changes. It is worth noting that gingival overgrowth induced by fixed orthodontic therapy, in most of the cases examined, co-exists with favouring factors that amplify its severity. In our study, the favouring factors were bacterial plaque and calculus accumulation, sharp-edged odontal lesions, marginally ill-fitting prosthetic restorations or massive unfinished crown fillings. Therefore, removing the contributing factors can help to improve the symptoms but also to reverse the inflammatory phenomena.

Globally, lung cancer is the primary cause of cancer-related mortality. Squamous cell carcinoma (SCC) of the lung, a subtype of non-small cell lung cancer (NSCLC), accounts for a significant portion of new cases and is primarily associated with smoking and environmental pollutants. This study investigates the histopathological and immunohistochemical (IHC) characteristics of the tumor microenvironment (TME) in SCC using tissue samples from 19 patients who underwent surgery for lung cancer. IHC markers, including cluster of differentiation (CD)68, CD3 and CD34 were used to assess various components of the TME, including immune cell infiltration, and angiogenesis. The results revealed significant presence of macrophages, T-lymphocytes, and myofibroblasts, as well as increased vascularization in the tumor stroma. These findings highlight the complex interaction between tumor cells and the surrounding stroma, contributing to tumor progression. Understanding these mechanisms is crucial for improving early diagnosis and therapeutic strategies for SCC.

Vaccination against Human Papilloma Virus continues to remain a highly complex public health issue, as there are many countries or regions that show increasing reluctance. Romania is among the countries that do not provide HPV vaccine in the national vaccination scheme, but offers the opportunity to young girls between 11 and 18 years of age to benefit from free vaccination. It is the responsibility of health professionals in all health sectors to inform themselves correctly, but also to instruct patients in a prophylactic approach against infection with both HPV and other types of microorganisms. Accurate information and a thorough knowledge of the prophylactic means are achieved from the years of study. Our study focuses on the following aspects: the degree of vaccination among dental students, the willingness of students to get vaccinated if they have not done it so far, the degree of information about Human Papilloma Virus infection, including ways of transmission and methods of diagnosing the infection, the perception of students on how this topic has been promoted, the sources of information they access in order to obtain information about Human Papilloma Virus.

As a science, facial and dental aesthetics is highly complex, the dentist being compelled to effectively exploit his abilities of reconstructing affected tissues, but also cultivate his aesthetic sense. Nowadays, dentists frequently face the patients high expectations, regarding dental structure and facial reconstruction. Most young patients tend to choose their model from the media, while patients over the age of 50 desire to have teeth like those of youth. It should also be noted that there is no concept of „cosmetic dentistry” as the teeth can only be adjusted in corellation to the face and body appearance. Thus, between physiognomy and the general appearance of the body and of the teeth a state of interdependence is in existence. One of the major errors that leads to endangering the quality of dental treatment and to patient dissatisfaction is the way in which the doctor-patient relationship is managed. If a person opts for an aesthetic treatment it is clear that it is facing a low self-esteem, in this case the dentist needing to address the disorder on two coordinates: an objective interpretation involves the sequencing of reconstructing a morphological and functional dentition and and establishing the issues related to patients notion of „beautiful”. At the same time, aesthetic dental treatment is based on the personality of the individual, the age, sex and patient's systemic diseases. The notion of „divine proportion” makes possible to perform „perfect” dental works that integrate the facial and somatic features, whose basic characteristics are symmetry, balance, continuity.

The papillomatous lesion has a number of implications that destabilize the normal functioning of the stomatognathic system, but also it has psychological implications. A patient with a papilloma located in an area of maximum visibility tends to be less exposed in public, to reduce socialization, to feel embarrassed. Oral papilloma affects the masticatory function, phonation, but also aesthetics. Understanding how local immunity works in patients diagnosed with oral papilloma is essential. Local immunity influences the evolution of the papillomatous oral lesion. Toll-like receptors are extremely important in the immune process of oral papilloma. Toll-like receptors can be used as indicators of lesion progression. A reduced expression of these receptors in the focus of the lesion is suggestive of pathological progress. It is also emphasized that in the oral cavity we find a well-represented local immune system which cells have an immune role and whose expression influences the evolution of the disease. The most incriminating factor in the occurrence of oral papilloma is HPV infection. The persistent inflammatory process produced by the HPV virus stimulates the development of oral, pharyngeal, laryngeal neoplasms. The HPV virus acts as a direct inducer in the process of transforming the benign lesion into a malignant lesion. Macrophages play an important role in the malignancy potential induced by the HPV virus. Macrophages are activated by increasing the metabolic rate and fighting tumor cells.