Hydrolyzed collagen (HC) is a group of peptides with low molecular weight (3–6 KDa) that can be obtained by enzymatic action in acid or alkaline media at a specific incubation temperature. HC can be extracted from different sources such as bovine or porcine. These sources have presented health limitations in the last years. Recently research has shown good properties of the HC found in skin, scale, and bones from marine sources. Type and source of extraction are the main factors that affect HC properties, such as molecular weight of the peptide chain, solubility, and functional activity. HC is widely used in several industries including food, pharmaceutical, cosmetic, biomedical, and leather industries. The present review presents the different types of HC, sources of extraction, and their applications as a biomaterial.
SARS-CoV-2 is an emerging infectious disease of zoonotic origin that caused the coronavirus disease in late 2019 and triggered a pandemic that has severely affected human health and caused millions of deaths. Early and massive diagnosis of SARS-CoV-2 infected patients is the key to preventing the spread of the virus and controlling the outbreak. Lateral flow immunoassays (LFIA) are the simplest biosensors. These devices are clinical diagnostic tools that can detect various analytes, including viruses and antibodies, with high sensitivity and specificity. This review summarizes the advantages, limitations, and evolution of LFIA during the SARS-CoV-2 pandemic and the challenges of improving these diagnostic devices.
Due to the increase in contamination of aquatic niches by different heavy metals, different technologies have been studied to eliminate these pollutants from contaminated aquatic sources. So the objective of this work was to determine the removal of cobalt (II) in aqueous solution by the biomass of the aquatic lily or water hyacinth (Eichhornia crassipes) which, is one of the main weeds present in fresh water, due to its rapid reproduction, growth, and high competitiveness, by the colorimetric method of the methyl isobutyl ketone. The removal was evaluated at different pHs (4.0–8.0) for 28 h. The effect of temperature in the range from 20 °C to 50 °C and the removal at different initial concentrations of cobalt (II) of 100 to 500 mg/L was also studied. The highest bioadsorption (100 mg/L) was at 28 h, at pH 5.0 and 28 °C, with a removal capacity of 73.1%, which is like some reports in the literature. Regarding the temperature, the highest removal was at 50 °C, at 28 h, with a removal of 89%. At the metal and biomass concentrations analyzed, its removal was 82% with 400–500 mg/L, and 100% with 5 g of natural biomass at 20 h. In addition, this completely removes the metal in situ (100 mg/L in contaminated water, at 7 days of incubation, with 10 g of natural biomass in 100 mL). So, the natural biomass can be used to remove it from industrial wastewater, even if in vivo, only eliminate 17.3% in 4 weeks.
The objective of this work was to study the removal of chromium (VI) in aqueous solution by the fungus Penicillium sp. IA-01, isolated from polluted air with industrial vapors. To obtain the fungal biomass, pre-inoculums were performed in thioglycolate broth from a strain isolated from vapours contaminated with Cr (VI). The fungus was incubated for four weeks at ambient temperature, filtered, and washed three times with trideionized water. In preparing cellullar fractions, it was necessary to break the fungal cells with glass beads using a homogenizer being careful to keep the samples in frosty cold ice. To obtain the fungal biomass, the fungus was filtered and stored in an oven at 80°C, allowing it to dry for 48 h. Removal of Cr (VI) in vitro was evaluated using different cellular fractions and dead fungal biomass. We determine the optimal characteristics for metal removal in the reaction mixture. Concluding that the ideal conditions for the removal of Cr (VI) in the cell extracts were 37°C and pH 7.0, also we observ that the highest enzyme activity was in the mixed membrane fraction. In dead fungal biomass, the ideal conditions for removal of metal are 60°C and pH 1.0.
Wild boars (Sus scrofa) were introduced in Mexico for sport hunting and meat trading for human consumption, but the available data regarding their role in pathogen transmission are limited. This research and field work aimed to identify the helminths of the wild boar produced in three units of conservation management and sustainable use of wildlife placed in the eastern economic region of Mexico. Samples of feces and serum were collected from 90 animals that came from three different ranches. Stool examination and antibody determination to Fasciola hepatica, Taenia crassciceps, Ascaris suum, Toxocara canis (ELISA), and Trichinella spiralis (Western blot) were performed. In addition, 30 diaphragm samples from one ranch were obtained for artificial digestion. Eggs of Strongyloides sp. (72.2%), Metastrongylus sp. (57.7%), Oesophagostomum sp. (53.3%), and Trichuris sp. (37.7%) were found in addition to oocysts of Eimeria sp. (75.6%). Antibodies to Fasciola (8.9%), Taenia (4.4%), Ascaris (32.2%), Toxocara (20%), and Trichinella (5.5%) were found. The eggs of Strongyloides and Oesophagostomum were associated to female hosts. One nematode larva was found by artificial digestion. This is the first report to identify helminths from wild boars in Mexico. In addition, this study identifies the potential risk of the wild boar as a transmission channel of parasites that can have an impact on public health.
The genus Aeromonas is a group of bacteria that is widely distributed in water bodies and belongs to the normal intestinal microbiota of aquatic and terrestrial animals. In the present work, rainbow trout fingerlings were experimentally infected by an immersion bath with different Aeromonas species. Subsequently, the behavior of the infected groups was observed and recorded. Infected fingerlings were evaluated by histopathology. The highest percentages of hyperpigmentation (18.88%) and inappetence (47.7%) were observed in fish infected with A. salmonicida, while abnormal swimming (83.33%) was recorded in fish infected with A. bestiarum. In histopathological findings, the highest percentages were observed in the olfactory epithelium (50.0%) for A. lusitana and A. salmonicida (41.1%)-infected fish. While, in the nervous system, the cerebral hemispheres (31.1%) in A. media-infected fish and the oblongata medulla (40.0%) in the A. bestiarum-infected fish presented the highest percentages. Meanwhile, A. salmonicida and A. bestiarum have the highest pathogenicity and virulence based on the histopathological findings in the olfactory epithelium and nervous system. Due to the proximity of the olfactory epithelium with the nervous tissue, it is possible that the infection generated by the Aeromonas species and the histopathological findings in the nervous tissue are reflected in different behavioral changes that suggest differences in the pathogenicity and virulence of the bacteria.