Magnetic hyperthermia (MHT) is in the spotlight of nanomedical research for the treatment of cancer employing magnetic iron oxide nanoparticles and their intrinsic capability for heat dissipation under an alternating magnetic field (AMF). Herein we focus on the synthesis of iron oxide nanoflowers (Nfs) of different sizes (15 and 35 nm) and coatings (bare, citrate, and Rhodamine B) while comparing their physicochemical and magnetothermal properties. We encapsulated colloidally stable citrate coated Nfs, of both sizes, in thermosensitive liposomes via extrusion, and RhB was loaded in the lipid bilayer. All formulations proved hemocompatible and cytocompatible. We found that 35 nm Nfs, at lower concentrations than 15 nm Nfs, served better as nanoheaters for magnetic hyperthermia applications. In vitro, magnetic hyperthermia results showed promising therapeutic and imaging potential for RhB loaded magnetoliposomes containing 35 nm Nfs against LLC and CULA cell lines of lung adenocarcinoma.

Herein, thermo-responsive liposomes (TLs) loaded with Asp (Asp/TLs) were produced by self-assembling DPPC, DSPE-PEG2000, and cholesterol. The preparation variables were optimized using the Box–Behnken design (BBD). The optimized Asp/TLs exhibited an average particle size of 114.05 ± 1.56 nm, PDI of 0.15 ± 0.015, zeta potential of −15.24 ± 0.65 mV, and entrapment efficiency (EE%) of 84.08 ± 2.75%. In addition, under physiological conditions, Asp/TLs showed spherical shape, outstanding stability and thermo-triggered the release of Asp at 38 °C, reaching the maximum Asp release at 40 °C. The MTT assay showed that the optimal Asp/TLs exhibited the highest cytotoxic activity upon exposure to mild hyperthermia (40 °C) against the invasive triple-negative breast cancer cell line (MDA-MB-231) when compared to other preparations. The IC₅₀ of Asp/TLs (40 °C) was estimated at 0.9 μg mL⁻¹, while that of free Asp (40 °C) was 3.83 μg mL⁻¹. As such, the optimal Asp/TLs were shown to increase the cytotoxic activity of Asp by 4-fold upon exposure to mild hyperthermia. The IC₅₀ values of Asp and Asp/TLs without exposure to 40 °C were 6.6 μg mL⁻¹ and 186 μg mL⁻¹, respectively. This indicated that Asp was released only when placed at 40 °C. The apoptosis assay revealed that Asp/TLs (40 °C) caused a remarkable increase in the percentage of cell population among both the late apoptosis and necrosis quartiles, as well as a significant decline in the viable cell quartile (P ≤ 0.001) when compared to Asp (40 °C). Asp/TLs (40 °C) and Asp (40 °C) could stimulate the intrinsic apoptosis pathway by upregulating the apoptotic genes Bak and Bax, while downregulating the anti-apoptotic genes, BCL-xL and BCL-2. The free Asp (40 °C) increased the gene expression of Bak and Bax by 4.4- and 5.2-folds, while reducing the expression of BCL-xL and BCL-2 by 50% and 73%, respectively. The optimal Asp TLs (40 °C) manifested more potent effects as demonstrated by the upregulation of Bak, Bax, and P53 by 5.6-, 7.2-, and 1.3-folds, as well as the downregulation of BCL-xL and BCL-2 by 70% and 85%, respectively. As such, the optimal Asp TLs (40 °C) treatment displayed the most potent cytotoxic profile and induced both apoptosis and necrosis in MDA-MB-231.

Optical and photocatalytic restrictions of anatase TiO₂ nanoparticles (Nps) limit their potential applications, as antipollutant and antibacterial agents for sanitary applications, to the UV spectral region. While modification with transition metals extends the absorption capacity to the visible light spectrum, often undermines the photocatalysts' biocompatibility due to toxic ion leaching. In this study, we synthesized Cu-doped and Ag-decorated TiO₂ photocatalysts by employing solvothermal (_ATiO₂:Cu) and sol-gel synthetic procedures (_BTiO₂:Ag), respectively. We acquired TiO₂ Nps modified with three percentages of either Cu or Ag content, to examine the potential differentiation of their structural, photocatalytic, and biological impact. Comprehensive structural characterization supports the prevailing anatase crystalline structure of bare and modified titania nanostructures, while morphological differences are demonstrated among the different samples. Optical response in the visible region of _ATiO₂:Cu Nps stems from band gap narrowing and lattice-defect generation, while plasmonic effects are at play for _BTiO₂:Ag Nps. Their photocatalytic potential under visible light irradiation, originated from low-energy LED lamps commonly found in indoor spaces, was verified after monitoring the successful enhancement of methylene blue (MB) degradation rate. Safety assessment on immortalized healthy human keratinocyte cell line (HaCaT) revealed their biocompatibility up to a certain concentration, while reactive oxygen species (ROS) production was intensified after light irradiation. The visible-light-induced photocatalytic-driven antibacterial activity was confirmed against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli.