An electrochemical immunosensor employs antibodies as capture and detection means to produce electrical charges for the quantitative analysis of target molecules. This sensor type can be utilized as a miniaturized device for the detection of point-of-care testing (POCT). Achieving high-performance analysis regarding sensitivity has been one of the key issues with developing this type of biosensor system. Many modern nanotechnology efforts allowed for the development of innovative electrochemical biosensors with high sensitivity by employing various nanomaterials that facilitate the electron transfer and carrying capacity of signal tracers in combination with surface modification and bioconjugation techniques. In this review, we introduce novel nanomaterials (e.g., carbon nanotube, graphene, indium tin oxide, nanowire and metallic nanoparticles) in order to construct a high-performance electrode. Also, we describe how to increase the number of signal tracers by employing nanomaterials as carriers and making the polymeric enzyme complex associated with redox cycling for signal amplification. The pros and cons of each method are considered throughout this review. We expect that these reviewed strategies for signal enhancement will be applied to the next versions of lateral-flow paper chromatography and microfluidic immunosensor, which are considered the most practical POCT biosensor platforms.

Clustering is an important means of data mining based on separating data categories by similar features. Unlike the classification algorithm, clustering belongs to the unsupervised type of algorithms. Two representatives of the clustering algorithms are the K-means and the expectation maximization (EM) algorithm. Linear regression analysis was extended to the category-type dependent variable, while logistic regression was achieved using a linear combination of independent variables. To predict the possibility of occurrence of an event, a statistical approach is used. However, the classification of all data by means of logistic regression analysis cannot guarantee the accuracy of the results. In this paper, the logistic regression analysis is applied to EM clusters and the K-means clustering method for quality assessment of red wine, and a method is proposed for ensuring the accuracy of the classification results.

In this study, the development of a deep learning approach for distinguishing cardiomegaly in chest X-ray images and its validation process are presented. Typically, radiologists diagnose cardiomegaly by examining X-ray images. However, their interpretations can vary owing to subjective judgments, and mild cardiomegaly can be missed. For this reason, there is ongoing research into the use of AI-based deep learning algorithms as an adjunct to X-ray interpretation. In this study, radiologists collected 10,000 public images, from which 718 useful images were selected to create a dataset. A DenseNet121 algorithm was then used to develop an AI model for cardiomegaly detection. The results demonstrate an accuracy of 0.95, a recall of 0.91, and an F1 score of 0.94. Additional validation was performed to ensure the accuracy of the cardiomegaly detection model. The validation methods included saliency maps and guided backpropagation, which indicated the significance of the model. In conclusion, this study demonstrates the potential to develop a high-quality AI model with fewer data than previous studies, suggesting its applicability in the medical field.

Objective The primary objective of this study was to predict subgroups of autism spectrum disorder (ASD) based on the Diagnostic Statistical Manual for Mental Disorders-IV Text Revision (DSM-IV-TR) by machine learning (ML). The secondary objective was to set up a ranking of Autism Diagnostic Interview-Revised (ADI-R) diagnostic algorithm items based on ML, and to confirm whether ML can sufficiently predict the diagnosis with these minimum items.Methods In the first experiment, a multiclass decision forest algorithm was applied, and the diagnostic algorithm score value of 1,269 Korean ADI-R test data was used for prediction. In the second experiment, we used 539 Korean ADI-R case data (over 48 months with verbal language) to apply mutual information to rank items used in the ADI diagnostic algorithm.Results In the first experiment, the results of predicting in the case of pervasive developmental disorder not otherwise specified as “ASD” were almost three times higher than predicting it as “No diagnosis.” In the second experiment, the top 10 ranking items of ADI-R were mainly related to the quality abnormality of communication.Conclusion In conclusion, we verified the applicability of ML in diagnosis and found that the application of artificial intelligence for rapid diagnosis or screening of ASD patients may be useful.

Content-based image retrieval (CBIR) focuses on video searching with fine-tuning of pre-trained off-the-shelf features. CBIR is an intuitive method for image retrieval, although it still requires labeled datasets for fine-tuning due to the inefficiency caused by annotation. Therefore, we explored an unsupervised model for feature extraction of image contents. We used a variational auto-encoder (VAE) expanding channel of neural networks and studied the activation of layer outputs. In this study, the channel expansion method boosted the capability of image retrieval by exploring more kernels and selecting a layer of comparatively activated object region. The experiment included a comparison of channel expansion and visualization of each layer in the encoder network. The proposed model achieved (52.7%) mAP, which outperformed (36.5%) the existing VAE on the MNIST dataset.

Sleep apnea has emerged as a significant health issue in modern society, with self-diagnosis and effective management becoming increasingly important. Among the most renowned methods for self-diagnosis, the STOP-BANG questionnaire is widely recognized as a simple yet effective tool for diagnosing and assessing the risk of sleep apnea. However, its sensitivity and specificity have limitations, necessitating the need for tools with higher performance. Consequently, this study aimed to enhance the accuracy of sleep apnea diagnoses by integrating machine learning with the STOP-BANG questionnaire. Research through actual cases was conducted based on the data of 262 patients undergoing polysomnography, confirming sleep apnea with a STOP-BANG score of ≥3 and an Apnea–Hypopnea Index (AHI) of ≥5. The accuracy, sensitivity, and specificity were derived by comparing Apnea–Hypopnea Index scores with STOP-BANG scores. When applying machine learning models, four hyperparameter-tuned models were utilized: K-Nearest Neighbor (K-NN), Logistic Regression, Random Forest, and Support Vector Machine (SVM). Among them, the K-NN model with a K value of 11 demonstrated superior performance, achieving a sensitivity of 0.94, specificity of 0.85, and overall accuracy of 0.92. These results highlight the potential of combining traditional STOP-BANG diagnostic tools with machine learning technology, offering new directions for future research in self-diagnosis and the preliminary diagnosis of sleep-related disorders in clinical settings.