The aim of the study was to evaluate the use of three by-products as growing substrates for Hermetia illucens (Black Soldier Fly (BSF)) larvae: okara, maize distiller, brewer’s grains, and a control hen diet. The study focused on larval growth and bioconversion performance, production of methane by larvae and environmental burden of larvae production, using Life Cycle Assessment (LCA) on a lab scale. Chemical composition of substrates differed: okara had the highest crude protein and ether extract contents, while brewer’s grains showed the highest fiber content. Larvae fed on a hen diet and maize distiller exhibited the highest final weights (2.29 and 1.97 g, respectively). Larvae grown on okara showed the highest indexes for waste reduction and efficiency of conversion of the ingested feed. The BSF larvae did not produce any detectable traces of CH₄. LCA evaluation showed that larvae production on a hen diet resulted in the most impact for most of environmental categories, for the inclusion of soybean meal in the diet (for climate change, 5.79 kg CO₂ eq/kg dry larvae). Feed production activities resulted in the main contributions to environmental impact. In order to compare the larvae production obtained on all substrates, an environmental impact was attributed to okara and brewer’s grain through a substitution method, and, by this approach, the best sustainable product resulted from the larvae grown on the maize distiller.

A large-scale survey was conducted on 173 dairy goat farms in Northern Italy to provide an updated view on farm management practices and to investigate relations among management factors, herd traits and milk yield and quality with particular focus on milk somatic cell count (SCC) and milk fat/protein reversion syndrome. Monthly individual milk analyses (fat, protein and lactose percentages, SCC) and bulk tank milk analyses (fat, protein and lactose percentages, standard plate count (SPC), urea and casein) from 91 farms out of the 173 farms were collected for a year. Farming systems showed wide variability and were characterised by the coexistence of intensive farms adopting advanced technologies and extensive traditional ones. Average milk production was 1.25 kg/milking with high variability, significantly influenced by days in milk, month of kidding, herd size and parity. Out-of-season breeding was predicted to cause a slight reduction of milk production. The reversion syndrome between milk fat and protein contents affected more than half of samples. It worsened after 100 d of lactation and occurred earlier during lactation when kidding was in the late season (April–June) compared to kidding in the first months of the year. The defect was associated with high SCC in milk. The yearly average milk SCC was 5.8 log₁₀ cells/mL; it was higher at the end of lactation and in goats kidding in the late season. A negative relationship between SCC and milk yield was found with an estimated loss of 0.23 kg per milking for each additional point of log₁₀ SCC.

Eight lactating Italian Friesian cows were housed in individual respiration chambers in a repeated Latin square design to determine their dry matter intake (DMI) and their milk and methane production, as well as to collect the total feces and urine to determine the N and energy balances. Four diets, based on the following forages (% of dry matter, DM), were tested: corn silage (CS, 49.3), alfalfa silage (AS, 26.8), wheat silage (WS, 20.0), and a typical hay-based Parmigiano Reggiano cheese production diet (PR, 25.3 of both alfalfa and Italian ryegrass hay). The greatest DMI was observed for cows fed PR (23.4 vs. 20.7 kg/d, the average of the other 3 diets). The DM digestibility was lower for PR (64.5 vs. 71.7%, the average of the other diets). The highest ash-free neutral detergent fiber digestibility values were obtained for CS (50.7%) and AS (47.4%). In the present study, no differences in milk production were observed between diets, although PR showed a higher milk yield trend. The highest milk urea N concentration (mg/dL) was found for the cows fed the WS diet (13.8), and the lowest was observed for the cows fed AS (9.24). The highest milk urea N concentration for the cows fed WS was also correlated with the highest urinary N excretion (g/d), which was found for the cows fed that same diet (189 vs. 147 on average for the other diets). The protein digestibility was higher for the cows fed the CS and WS diets (on average 68.5%) than for the cows fed AS and PR (on average 57.0%); dietary soybean inclusion was higher for CS and WS than for AS and PR. The rumen fermentation pattern was affected by the diet; the cows fed the PR diet showed a higher rumen pH and decreased propionate production than those fed CS, due to the lower nonfiber carbohydrate content and higher ash-free neutral detergent fiber content of the PR diet than the CS diet. Feeding cows with PR diet increased the acetate:propionate ratio in comparison with the CS diet (3.30 vs. 2.44 for PR and CS, respectively). Cows fed the PR diet produced a greater daily amount of methane and had a greater methane energy loss (% of digestible energy intake) than those fed the CS diet (413 vs. 378 g/d and 8.67 vs. 7.70%), but no differences were observed when methane was expressed as grams per kilogram of DMI or grams per kilogram of milk. The PR diet resulted in a smaller net energy for lactation content than the CS diet (1.36 vs. 1.70 Mcal/kg of DM for the PR and CS diets, respectively). Overall, our research suggests that a satisfactory milk production can be attained by including different high-quality forages in balanced diets without any negative effect on milk production or on the methane emissions per kilogram of milk.

One popular methodology for assessing the environmental impact of livestock sector is Life Cycle Assessment (LCA), that quantifies the environmental impact of a product. Ecological Footprint (EF) performs an environmental sustainability assessment, by comparing the demand for natural capital by an economic activity with the offer of such capital within a certain territory. The aim of the study was the comparison between LCA and EF in assessing the environmental performances of milk production, assuming as case study three cattle farms with increasing levels of production intensity. Different metrics and functional units (FU) (i.e., fat and protein corrected milk, FPCM and hectare) were adopted for LCA analysis, considering some of the major impact categories. For greenhouse gases emissions, the Global Warming Potential (GWP) and the Global Temperature Potential (GTP) were considered. Both metrics were calculated assuming or not the distinction between biogenic and fossil methane. Adopting GWP as a metric, the results per kg of FPCM provided by the LCA highlighted a different trade off compared to the EF method: the farm with the highest productive intensity produced the least impactful milk in terms of GWP but had the most negative Ecological Balance (EB). The same occurred for the other impact categories. When GTP was adopted, or the hectare was considered as FU, the least intensive farm, characterized by greater feed self-sufficiency, became the one that produced the least impactful milk and had the least negative EB. The study highlighted the scientific significance of the integration between the two approaches for creating a comprehensive representation of the effects of human activities on the environment. The LCA method evaluates impacts intensity referred to a specific functional unit and its results are strongly influenced by productive efficiency; the EF method evaluates environmental sustainability of productions in relation to the territory that supports them.