This page has only limited features, please log in for full access.
Honeybee and general pollinator decline is extensively reported in many countries, adding new concern to the general biodiversity loss. Many studies were addressed to assess the causes of pollinator decline, concluding that in most cases multi-stress effects were the most probable ones. In this research, the combined effects of two possible stress sources for bees, pesticides and electromagnetic fields (multi-stress conditions), were analyzed in the field. Three experimental sites were chosen: a control one far from direct anthropogenic stress sources, a pesticide-stress site and multi-stress one, adding to the same exposure to pesticides the presence of an electromagnetic field, coming from a high-voltage electric line. Experimental apiaries were monitored weekly for one year (from April 2017 to April 2018) by means of colony survival, queen activity, storage and brood amount, parasites and pathogens, and several biomarkers in young workers and pupae. Both exposure and effect biomarkers were analysed: among the first, acetylcholinesterase (AChE), catalase (CAT), glutathione S-transferase (GST) and alkaline phosphatase (ALP) and Reactive Oxygen Species (ROS); and among the last, DNA fragmentation (DNAFRAGM) and lipid peroxidation (LPO). Results showed that bee health conditions were the worst in the multi-stress site with only one colony alive out of the four ones present at the beginning. In this site, a complex picture of adverse effects was observed, such as disease appearance (American foulbrood), higher mortality in the underbaskets (common to pesticide-stress site), behavioral alterations (queen changes, excess of honey storage) and biochemical anomalies (higher ALP activity at the end of the season). The overall results clearly indicate that the multi-stress conditions were able to induce biochemical, physiological and behavioral alterations which severely threatened bee colony survival.
Daniela Lupi; Marco Palamara Mesiano; Agnese Adani; Roberto Benocci; Roberto Giacchini; Paolo Parenti; Giovanni Zambon; Antonio Lavazza; Maria Boniotti; Stefano Bassi; Mario Colombo; Paolo Tremolada. Combined Effects of Pesticides and Electromagnetic-Fields on Honeybees: Multi-Stress Exposure. Insects 2021, 12, 716 .
AMA StyleDaniela Lupi, Marco Palamara Mesiano, Agnese Adani, Roberto Benocci, Roberto Giacchini, Paolo Parenti, Giovanni Zambon, Antonio Lavazza, Maria Boniotti, Stefano Bassi, Mario Colombo, Paolo Tremolada. Combined Effects of Pesticides and Electromagnetic-Fields on Honeybees: Multi-Stress Exposure. Insects. 2021; 12 (8):716.
Chicago/Turabian StyleDaniela Lupi; Marco Palamara Mesiano; Agnese Adani; Roberto Benocci; Roberto Giacchini; Paolo Parenti; Giovanni Zambon; Antonio Lavazza; Maria Boniotti; Stefano Bassi; Mario Colombo; Paolo Tremolada. 2021. "Combined Effects of Pesticides and Electromagnetic-Fields on Honeybees: Multi-Stress Exposure." Insects 12, no. 8: 716.
The aim of this work was to develop processing methods that safeguard the quality and antimicrobial properties of H. illucens and B. mori oils. We adopted a vegetable diet for both insects: leftover vegetables and fruit for H. illucens and mulberry leaves for B. mori. First, alternative techniques to obtain a good oil extraction yield from the dried biomass of H. illucens larvae were tested. Traditional pressing resulted to be the best system to maximize the oil yield and it was successfully applied to B. mori pupae. Oil quality resulted comparable to that obtained with other extraction methods described in the literature. In the case of B. mori pupae, different treatments and preservation periods were investigated to evaluate their influence on the oil composition and quality. Interestingly, agar diffusion assays demonstrated the sensitivity of Gram-positive Bacillus subtilis and Staphylococcus aureus to H. illucens and B. mori derived oils, whereas the growth of Gram-negative Pseudomonas aeruginosa and Escherichia coli was not affected. This study confirms that fat and other active compounds of the oil extracted by hot pressing could represent effective antimicrobials against bacteria, a relevant result if we consider that they are by-products of the protein extraction process in the feed industry.
Alessio Saviane; Luca Tassoni; Daniele Naviglio; Daniela Lupi; Sara Savoldelli; Giulia Bianchi; Giovanna Cortellino; Paolo Bondioli; Liliana Folegatti; Morena Casartelli; Viviana Orlandi; Gianluca Tettamanti; Silvia Cappellozza. Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity. Animals 2021, 11, 783 .
AMA StyleAlessio Saviane, Luca Tassoni, Daniele Naviglio, Daniela Lupi, Sara Savoldelli, Giulia Bianchi, Giovanna Cortellino, Paolo Bondioli, Liliana Folegatti, Morena Casartelli, Viviana Orlandi, Gianluca Tettamanti, Silvia Cappellozza. Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity. Animals. 2021; 11 (3):783.
Chicago/Turabian StyleAlessio Saviane; Luca Tassoni; Daniele Naviglio; Daniela Lupi; Sara Savoldelli; Giulia Bianchi; Giovanna Cortellino; Paolo Bondioli; Liliana Folegatti; Morena Casartelli; Viviana Orlandi; Gianluca Tettamanti; Silvia Cappellozza. 2021. "Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity." Animals 11, no. 3: 783.
A plant can combine physical and chemical tools to interact with other organisms. Some are designed for pollinator attraction (i.e., colors and volatile organic compounds-VOCs); others can act to discourage herbivores (i.e., non-glandular trichomes). Few studies fully address available tools in a single species; notwithstanding, this information can be pivotal in understanding new interactions out of the home range. We characterized flower traits, emission profiles of constitutive compounds from flowers and leaves, micro-morphology of the glandular trichomes, and listed flower visitors of two Mexican bird-pollinated Salvia species (S. blepharophylla and S. greggii), growing in an Italian botanical garden. Flowers were highly variable in their morphometric characteristics. In both species, four trichome morphotypes with similar histochemistry and distribution were documented for leaves and flowers except the calyx abaxial side. The vegetative emission profiles were qualitatively more complex than the floral ones; however, common compounds occurring in high relative percentages were β-caryophyllene and germacrene D. Floral bouquets were dominated by limonene and β-pinene in S. greggii and by 1,8-cineole in S. blepharophylla. Two potential (non-bird) pollinators were especially abundant: small bees belonging to the genus Lasioglossum and large bees belonging to the species Xylocopa violacea. Our study highlights the plasticity of these plants, as well as tools that can be conveniently used to establish novel interactions.
Claudia Giuliani; Manuela Giovanetti; Daniela Lupi; Marco Palamara Mesiano; Renata Barilli; Roberta Ascrizzi; Guido Flamini; Gelsomina Fico. Tools to Tie: Flower Characteristics, VOC Emission Profile, and Glandular Trichomes of Two Mexican Salvia Species to Attract Bees. Plants 2020, 9, 1645 .
AMA StyleClaudia Giuliani, Manuela Giovanetti, Daniela Lupi, Marco Palamara Mesiano, Renata Barilli, Roberta Ascrizzi, Guido Flamini, Gelsomina Fico. Tools to Tie: Flower Characteristics, VOC Emission Profile, and Glandular Trichomes of Two Mexican Salvia Species to Attract Bees. Plants. 2020; 9 (12):1645.
Chicago/Turabian StyleClaudia Giuliani; Manuela Giovanetti; Daniela Lupi; Marco Palamara Mesiano; Renata Barilli; Roberta Ascrizzi; Guido Flamini; Gelsomina Fico. 2020. "Tools to Tie: Flower Characteristics, VOC Emission Profile, and Glandular Trichomes of Two Mexican Salvia Species to Attract Bees." Plants 9, no. 12: 1645.
Many species of long-horned beetles are invasive pests causing significant economic damage in agro-forestry systems. They spend the majority of their life-cycle concealed inside natural wood or wooden packaging materials and are largely protected from adverse environmental conditions and pesticide sprays. Biological control via parasitoid natural enemies including members of the bethylid genus Sclerodermus, has proven effective against some long-horned beetles that are invasive in China. In Europe, the biocontrol potential of native Sclerodermus species is being evaluated with a view to developing efficient mass-rearing techniques and then actively deploying them against invasive Asian beetles. Here, we continue evaluations of S. brevicornis by establishing that groups of females that have already reared offspring to emergence are capable of reproducing subsequent hosts and by evaluating the lifetime reproductive capacity of individual females provided with successive hosts. Additionally, we assess the laboratory shelf-life of adult females stored for different times at different temperatures including cold storage, and then assess the post-storage reproductive performance of groups of females provided with a single host. We found that adult female longevity declines with increasing storage temperature and that most aspects of subsequent performance are negatively affected by high temperatures. The adaptability to low temperature storage enhances the suitability of S. brevicornis to mass-rearing programs and thus biocontrol deployment.
Costanza Jucker; Ian C.W. Hardy; Serena Malabusini; Silvia De Milato; Giacomo Zen; Sara Savoldelli; Daniela Lupi. Factors Affecting the Reproduction and Mass-Rearing of Sclerodermus brevicornis (Hymenoptera: Bethylidae), a Natural Enemy of Exotic Flat-Faced Longhorn Beetles (Coleoptera: Cerambycidae: Lamiinae). Insects 2020, 11, 657 .
AMA StyleCostanza Jucker, Ian C.W. Hardy, Serena Malabusini, Silvia De Milato, Giacomo Zen, Sara Savoldelli, Daniela Lupi. Factors Affecting the Reproduction and Mass-Rearing of Sclerodermus brevicornis (Hymenoptera: Bethylidae), a Natural Enemy of Exotic Flat-Faced Longhorn Beetles (Coleoptera: Cerambycidae: Lamiinae). Insects. 2020; 11 (10):657.
Chicago/Turabian StyleCostanza Jucker; Ian C.W. Hardy; Serena Malabusini; Silvia De Milato; Giacomo Zen; Sara Savoldelli; Daniela Lupi. 2020. "Factors Affecting the Reproduction and Mass-Rearing of Sclerodermus brevicornis (Hymenoptera: Bethylidae), a Natural Enemy of Exotic Flat-Faced Longhorn Beetles (Coleoptera: Cerambycidae: Lamiinae)." Insects 11, no. 10: 657.
Environmental quality (e.g., diversity of resource availability, nesting sites, environmental display) plays an important role in an animal’s life. While homogeneous environments can restrict organisms from developing activities such as food seeking (behavioral impairment), more complex environments allow animals to perform activities with learning and behavioral perfecting outcomes. Pesticides are known to affect the learning and foraging behaviors of bees; however, little is known about the counterbalance displayed by the environment. Herein, we conducted two experiments that simulated distinct environmental displays, in which the effects of a fungicide (IndarTM 5EW-febunconazole) on solitary bee foraging activities were tested. We found that the fungicide only impaired the activities of bees in one of the studied environments. The difference in visitation rates and flower exploitation of bees between the two different environmental displays led to changes in metrics of bee–flower networks across environments. Linkage density, a metric associated with pollination efficiency that is known to be impacted by different environments, differed across environments. Our results showed that ecological interaction network metrics can differ regarding the different environmental displays. This study indicates that environmental complexity helps balance the negative effects of pesticides on solitary bees and highlights the potential use of solitary bees as model organisms for experimental simulations of environmental change.
Samuel Boff; Josué Raizer; Daniela Lupi. Environmental Display can Buffer the Effect of Pesticides on Solitary Bees. Insects 2020, 11, 417 .
AMA StyleSamuel Boff, Josué Raizer, Daniela Lupi. Environmental Display can Buffer the Effect of Pesticides on Solitary Bees. Insects. 2020; 11 (7):417.
Chicago/Turabian StyleSamuel Boff; Josué Raizer; Daniela Lupi. 2020. "Environmental Display can Buffer the Effect of Pesticides on Solitary Bees." Insects 11, no. 7: 417.
Sclerodermus brevicornis is a parasitoid that exhibits cooperative multi-foundress brood production. Prior work showed that the time lag to paralysis of small-sized hosts is shorter when co-foundress relatedness is higher and predicted that the greater risks and greater benefits of attacking larger hosts would combine with co-foundress relatedness to determine the limits to the size of a host that a female is selected to attack as a public good. It was also predicted that the time to host attack would be affected by an interaction between host size and relatedness. Here, we show empirically that both host size and kinship affect S. brevicornis reproduction and that they interact to influence the timing of host attack. We also find effects of co-foundress relatedness after hosts have been suppressed successfully. A public goods model using parameters estimated for S. brevicornis again suggests that selection for individual foundresses to attack and, if successful, to share hosts will be dependent on both the size of the host and the relatedness of the foundresses to any co-foundresses present. Females will not be selected to bear the individual cost of a public good when hosts are large and dangerous or when their relatedness to the co-foundress is low. We conclude that although reproductive behaviours exhibited by Sclerodermus females can be cooperative, they are unlikely to be exhibited without reference to kinship or to the risks involved in attempting to suppress and share large and dangerous hosts.
Mohamed Khadar Abdi; Ian C W Hardy; Costanza Jucker; Daniela Lupi. Kinship effects in quasi-social parasitoids II: co-foundress relatedness and host dangerousness interactively affect host exploitation. Biological Journal of the Linnean Society 2020, 130, 642 -660.
AMA StyleMohamed Khadar Abdi, Ian C W Hardy, Costanza Jucker, Daniela Lupi. Kinship effects in quasi-social parasitoids II: co-foundress relatedness and host dangerousness interactively affect host exploitation. Biological Journal of the Linnean Society. 2020; 130 (4):642-660.
Chicago/Turabian StyleMohamed Khadar Abdi; Ian C W Hardy; Costanza Jucker; Daniela Lupi. 2020. "Kinship effects in quasi-social parasitoids II: co-foundress relatedness and host dangerousness interactively affect host exploitation." Biological Journal of the Linnean Society 130, no. 4: 642-660.
Explanations for the highest levels of sociality typically invoke the concept of inclusive fitness. Sclerodermus, a genus of parasitoid hymenopterans, is quasi-social, exhibiting cooperative brood care without generational overlap or apparent division of labour. Foundress females successfully co-exploit hosts that are too large to suppress when acting alone and the direct fitness benefits of collective action may explain their cooperation, irrespective of kinship. However, cooperation in animal societies is seldom free of conflicts of interest between social partners, especially when their relatedness, and thus their degree of shared evolutionary interests, is low. We screened components of the life-history of Sclerodermus brevicornis for effects of varying co-foundress number and relatedness on cooperative reproduction. We found that the time taken to paralyse standard-sized hosts is shorter when co-foundress number and/or relatedness is higher. This suggests that, while females must access a paralysed host in order to reproduce, individuals are reluctant to take the risk of host attack unless the benefits will be shared with their kin. We used Hamilton’s rule and prior data from studies that experimentally varied the sizes of hosts presented to congeners to explore how the greater risks and greater benefits of attacking larger hosts could combine with relatedness to determine the sizes of hosts that individuals are selected to attack as a public good. From this, we predict that host size and relatedness will interact to affect the timing of host paralysis; we test this prediction in the accompanying study.
Mohamed Khadar Abdi; Daniela Lupi; Costanza Jucker; Ian C W Hardy. Kinship effects in quasi-social parasitoids I: co-foundress number and relatedness affect suppression of dangerous hosts. Biological Journal of the Linnean Society 2020, 130, 627 -641.
AMA StyleMohamed Khadar Abdi, Daniela Lupi, Costanza Jucker, Ian C W Hardy. Kinship effects in quasi-social parasitoids I: co-foundress number and relatedness affect suppression of dangerous hosts. Biological Journal of the Linnean Society. 2020; 130 (4):627-641.
Chicago/Turabian StyleMohamed Khadar Abdi; Daniela Lupi; Costanza Jucker; Ian C W Hardy. 2020. "Kinship effects in quasi-social parasitoids I: co-foundress number and relatedness affect suppression of dangerous hosts." Biological Journal of the Linnean Society 130, no. 4: 627-641.
Biological control efficiency can be improved by developing effective mass‐rearing systems to produce large numbers of high‐quality parasitoids. This study explored an alternative host for rearing Sclerodermus brevicornis (Kieffer) (Hymenoptera: Bethylidae), a potential biocontrol agent for the suppression of exotic and invasive wood‐boring longhorn beetle (Coleoptera: Cerambycidae) populations in the European agroforestry ecosystems. We tested larvae of the rice moth, Corcyra cephalonica Stainton (Lepidoptera: Pyralidae), as host for the parasitoid. We quantified the probability and timing of host attack and parasitism as well as reproductive success, offspring production, and the characteristics of adult offspring. As S. brevicornis is a quasi‐social species (multiple females, communally produced offspring broods), we also explored the effects of varying the number of females to which individual hosts were presented, with the aim of determining the optimal female‐to‐host ratio. As time to host attack can be a limiting factor in S. brevicornis rearing protocols, we tested the use of adult females of another bethylid species, Goniozus legneri Gordh, to paralyse C. cephalonica larvae prior to presentation. We identified the conditions within our experiment that maximised offspring production per host and offspring production per adult female parasitoid. We found that C. cephalonica is suitable as a factitious host and, as it is considerably more straightforward for laboratory rearing than cerambycid species, it is a good candidate for adoption by future S. brevicornis mass‐rearing and release programmes.
Mohamed Khadar Abdi; Costanza Jucker; Beatrice De Marchi; Ian C.W. Hardy; Daniela Lupi. Performance of Sclerodermus brevicornis, a parasitoid of invasive longhorn beetles, when reared on rice moth larvae. Entomologia Experimentalis et Applicata 2020, 169, 64 -78.
AMA StyleMohamed Khadar Abdi, Costanza Jucker, Beatrice De Marchi, Ian C.W. Hardy, Daniela Lupi. Performance of Sclerodermus brevicornis, a parasitoid of invasive longhorn beetles, when reared on rice moth larvae. Entomologia Experimentalis et Applicata. 2020; 169 (1):64-78.
Chicago/Turabian StyleMohamed Khadar Abdi; Costanza Jucker; Beatrice De Marchi; Ian C.W. Hardy; Daniela Lupi. 2020. "Performance of Sclerodermus brevicornis, a parasitoid of invasive longhorn beetles, when reared on rice moth larvae." Entomologia Experimentalis et Applicata 169, no. 1: 64-78.
Kinship among interacting individuals is often associated with sociality and also with sex ratio effects. Parasitoids in the bethylid genus Goniozus are sub‐social, with single foundress females exhibiting post‐ovipositional maternal care via short‐term aggressive host and brood defence against conspecific females. Due to local mate competition (LMC) and broods normally being produced by a single foundress, sex ratios are female biased. Contests between adult females are, however, not normally fatal and aggression is reduced when competing females are kin, raising the possibility of multi‐foundress reproduction on some hosts. Here we screen for further life‐history effects of kinship by varying the numbers and relatedness of foundresses confined together with a host resource and also by varying the size of host. We confined groups of 1 to 8 Goniozus nephantidis females together with a host for 5+ days. Multi‐foundress groups were either all siblings or all non‐siblings. Our chief expectations included that that competition for resources would be more intense among larger foundress groups but diminished by both larger host size and closer foundress relatedness, affecting both foundress mortality and reproductive output. From classical LMC theory, we expected that offspring group sex ratios would be less female biased when there were more foundresses and from extended LMC theory we expected that sex ratios would be more female biased when foundresses were close kin. We found that confinement led to the death of some females (11% overall) but only when host resources were most limiting. Mortality of foundresses was less common when foundresses were siblings. Developmental mortality among offspring was considerably higher in multi‐foundress clutches but was unaffected by foundress relatedness. Groups of sibling foundresses collectively produced similar numbers of offspring to non‐sibling groups. There was little advantage for individual females to reproduce in multi‐foundress groups: single foundresses suppressed even the largest hosts presented and had the highest per capita production of adult offspring. Despite single‐foundress reproduction being the norm, G. nephantidis females in multi‐foundress groups appear to attune sex allocation according to both foundress number and foundress relatedness: broods produced by sibling foundresses had sex ratios similar to broods produced by single foundresses (ca. 11% males) whereas the sex ratios of broods produced by non‐sibling females were approximately 20% higher and broadly increased with foundress number. We conclude that relatedness and host size may combine to reduce selection against communal reproduction on hosts and that, unlike other studied parasitoids, G. nephantidis sex ratios conform to predictions of both classical and extended LMC theory.
Mohamed Khadar Abdi; Daniela Lupi; Ian C. W. Hardy. Co‐foundress confinement elicits kinship effects in a naturally sub‐social parasitoid. Journal of Evolutionary Biology 2020, 33, 1068 -1085.
AMA StyleMohamed Khadar Abdi, Daniela Lupi, Ian C. W. Hardy. Co‐foundress confinement elicits kinship effects in a naturally sub‐social parasitoid. Journal of Evolutionary Biology. 2020; 33 (8):1068-1085.
Chicago/Turabian StyleMohamed Khadar Abdi; Daniela Lupi; Ian C. W. Hardy. 2020. "Co‐foundress confinement elicits kinship effects in a naturally sub‐social parasitoid." Journal of Evolutionary Biology 33, no. 8: 1068-1085.
Life-sciences are pointing towards an alarming worldwide pollinator decline. This decline proceeds along with overall biodiversity losses, even in the context of urban landscapes and human welfare. At the same time, social-sciences are arguing an increased distance from nature, experienced by citizens. The strong connection between the public good and pollinator sustainability, even in urban areas, is well-documented. However, usually basic and applied life-sciences tend to underestimate public perception of nature, which is better tackled by the fields of social-sciences. Therefore, more efforts are needed to link scientific questions and public ‘perception’ of nature. We designed a transversal project where research questions directly confront public concerns: i.e., even while addressing scientific knowledge gaps, our questions directly arise from public concerns. Social studies highlighted that appreciation of (exotic) plants is related to the impact they may have on the surrounding natural environment: therefore, we investigated links of native and exotic flowers to local pollinators. Other studies highlighted that scientific results need to link to everyday individual experience: therefore, we investigated pollination modes of the renown Salvia, native and exotic, largely used in cuisine and gardening. The botanic garden was the promoter of scientific questions addressed by the public, and also collated the results in a travelling exhibition. The exhibition, together with a dedicated catalogue, were especially designed to enlighten the wide public on the relationships that plants, native and exotic alike, establish with the surrounding world.
Manuela Giovanetti; Claudia Giuliani; Samuel Boff; Gelsomina Fico; Daniela Lupi. A botanic garden as a tool to combine public perception of nature and life-science investigations on native/exotic plants interactions with local pollinators. PLOS ONE 2020, 15, e0228965 .
AMA StyleManuela Giovanetti, Claudia Giuliani, Samuel Boff, Gelsomina Fico, Daniela Lupi. A botanic garden as a tool to combine public perception of nature and life-science investigations on native/exotic plants interactions with local pollinators. PLOS ONE. 2020; 15 (2):e0228965.
Chicago/Turabian StyleManuela Giovanetti; Claudia Giuliani; Samuel Boff; Gelsomina Fico; Daniela Lupi. 2020. "A botanic garden as a tool to combine public perception of nature and life-science investigations on native/exotic plants interactions with local pollinators." PLOS ONE 15, no. 2: e0228965.
The effects of pesticide mixtures and electric and magnetic fields on honeybees were evaluated in three experimental sites located in northern Italy: a control site far from anthropogenic-stress sources, a semi-natural site close to a high-voltage electric line and an agricultural site with intensive pesticide use. From each experimental site, young workers and foraging bees were taken monthly from May to October and analyzed for four enzymatic biomarkers: acetylcholinesterase (AChE), catalase (CAT), glutathione S-transferase (GST) and alkaline phosphatase (ALP). The results revealed time- and site-specific effects in respect to control site, confirming the role of biomarkers as diagnostic and early-warning tools for multi-stress sources on honeybees. In the electromagnetic-stress site, the effect of an over-activation of all analyzed biomarkers was observed at the end of the season. According to other literature findings, this event was related to a behavioral over-activation in a period in which bees should prepare themselves for overwintering. This finding poses potential problems for winter survival. In the pesticide-stress site, different pesticide-induced responses were identified. We demonstrated in the field that pesticide mixtures currently used in agriculture could greatly affect the biochemical parameters of bees (with both enzymatic under- and over-activations).
Daniela Lupi; Paolo Tremolada; Mario Colombo; Roberto Giacchini; Roberto Benocci; Paolo Parenti; Marco Parolini; Giovanni Zambon; Marco Vighi. Effects of Pesticides and Electromagnetic Fields on Honeybees: A Field Study Using Biomarkers. International Journal of Environmental Research 2020, 14, 107 -122.
AMA StyleDaniela Lupi, Paolo Tremolada, Mario Colombo, Roberto Giacchini, Roberto Benocci, Paolo Parenti, Marco Parolini, Giovanni Zambon, Marco Vighi. Effects of Pesticides and Electromagnetic Fields on Honeybees: A Field Study Using Biomarkers. International Journal of Environmental Research. 2020; 14 (1):107-122.
Chicago/Turabian StyleDaniela Lupi; Paolo Tremolada; Mario Colombo; Roberto Giacchini; Roberto Benocci; Paolo Parenti; Marco Parolini; Giovanni Zambon; Marco Vighi. 2020. "Effects of Pesticides and Electromagnetic Fields on Honeybees: A Field Study Using Biomarkers." International Journal of Environmental Research 14, no. 1: 107-122.
Hermetia illucens is an efficient bioconverter able to grow on various different organic materials, producing larvae, which are a good source of protein and fat with applications in the animal feed and biochemical industries. This fly’s capacity to reduce huge amounts of waste presents an interesting opportunity to establish a circular food economy. In this study, we assessed the suitability of using organic wastes from cricket and locust farming to rear H. illucens. Larvae developed until adult emergence on all the wastes, with a mean survival of over 94%. Cricket waste allowed faster development of heavier larvae than locust waste. Substrate reduction was particularly interesting on cricket waste (
Costanza Jucker; Daniela Lupi; Christopher Douglas Moore; Maria Giovanna Leonardi; Sara Savoldelli. Nutrient Recapture from Insect Farm Waste: Bioconversion with Hermetia illucens (L.) (Diptera: Stratiomyidae). Sustainability 2020, 12, 362 .
AMA StyleCostanza Jucker, Daniela Lupi, Christopher Douglas Moore, Maria Giovanna Leonardi, Sara Savoldelli. Nutrient Recapture from Insect Farm Waste: Bioconversion with Hermetia illucens (L.) (Diptera: Stratiomyidae). Sustainability. 2020; 12 (1):362.
Chicago/Turabian StyleCostanza Jucker; Daniela Lupi; Christopher Douglas Moore; Maria Giovanna Leonardi; Sara Savoldelli. 2020. "Nutrient Recapture from Insect Farm Waste: Bioconversion with Hermetia illucens (L.) (Diptera: Stratiomyidae)." Sustainability 12, no. 1: 362.
The aim of the study was to evaluate the use of three by-products as growing substrates for Hermetia illucens (Black Soldier Fly (BSF)) larvae: okara, maize distiller, brewer's grains, and a control hen diet. The study focused on larval growth and bioconversion performance, production of methane by larvae and environmental burden of larvae production, using Life Cycle Assessment (LCA) on a lab scale. Chemical composition of substrates differed: okara had the highest crude protein and ether extract contents, while brewer's grains showed the highest fiber content. Larvae fed on a hen diet and maize distiller exhibited the highest final weights (2.29 and 1.97 g, respectively). Larvae grown on okara showed the highest indexes for waste reduction and efficiency of conversion of the ingested feed. The BSF larvae did not produce any detectable traces of CH4. LCA evaluation showed that larvae production on a hen diet resulted in the most impact for most of environmental categories, for the inclusion of soybean meal in the diet (for climate change, 5.79 kg CO2 eq/kg dry larvae). Feed production activities resulted in the main contributions to environmental impact. In order to compare the larvae production obtained on all substrates, an environmental impact was attributed to okara and brewer's grain through a substitution method, and, by this approach, the best sustainable product resulted from the larvae grown on the maize distiller.
Luciana Bava; Costanza Jucker; Giulia Gislon; Daniela Lupi; Sara Savoldelli; Maddalena Zucali; Stefania Colombini. Rearing of Hermetia Illucens on Different Organic By-Products: Influence on Growth, Waste Reduction, and Environmental Impact. Animals 2019, 9, 289 .
AMA StyleLuciana Bava, Costanza Jucker, Giulia Gislon, Daniela Lupi, Sara Savoldelli, Maddalena Zucali, Stefania Colombini. Rearing of Hermetia Illucens on Different Organic By-Products: Influence on Growth, Waste Reduction, and Environmental Impact. Animals. 2019; 9 (6):289.
Chicago/Turabian StyleLuciana Bava; Costanza Jucker; Giulia Gislon; Daniela Lupi; Sara Savoldelli; Maddalena Zucali; Stefania Colombini. 2019. "Rearing of Hermetia Illucens on Different Organic By-Products: Influence on Growth, Waste Reduction, and Environmental Impact." Animals 9, no. 6: 289.
The presence of the four exotic drosophilids Chymomyza amoena (Loew), Drosophila suzukii (Matsumura), Zaprionus indianus (Gupta) and Zaprionus tuberculatus Malloch has been investigated in different orchards in Northern Italy for two consecutive years. The presence and the abundance of the population of the drosophilid flies were surveyed with apple cider vinegar traps, fruit baited traps, and fruit collection. Chymomyza amoena, Z. tuberculatus and D. suzukii have been identified in the Apple Cider Vinegar traps in both years. Only D. suzukii and Z. tuberculatus emerged from fruit baited traps. Except for D. suzukii, no other exotic drosofilid was captured from the fruit collection. Z. indianus was never observed. Analyses of the presence of the different species, seasonal occurrence and sex ratio are provided.
N. Amiresmaeili; C. Jucker; S. Savoldelli; D. Lupi. Can exotic drosophilids share the same niche of the invasive Drosophila suzukii? Journal of Entomological and Acarological Research 2019, 51, 1 .
AMA StyleN. Amiresmaeili, C. Jucker, S. Savoldelli, D. Lupi. Can exotic drosophilids share the same niche of the invasive Drosophila suzukii? Journal of Entomological and Acarological Research. 2019; 51 (1):1.
Chicago/Turabian StyleN. Amiresmaeili; C. Jucker; S. Savoldelli; D. Lupi. 2019. "Can exotic drosophilids share the same niche of the invasive Drosophila suzukii?" Journal of Entomological and Acarological Research 51, no. 1: 1.
Costanza Jucker; Augusto Loni; Mattia Calzolari; Sergey Belokobylskij; Daniela Lupi. ACCIDENTAL INTRODUCTION IN ITALY OF THE PARASITOID SPATHIUS VULNIFICUS WILKINSON (HYMENOPTERA BRACONIDAE DORYCTINAE). Redia 2018, 189 -191.
AMA StyleCostanza Jucker, Augusto Loni, Mattia Calzolari, Sergey Belokobylskij, Daniela Lupi. ACCIDENTAL INTRODUCTION IN ITALY OF THE PARASITOID SPATHIUS VULNIFICUS WILKINSON (HYMENOPTERA BRACONIDAE DORYCTINAE). Redia. 2018; ():189-191.
Chicago/Turabian StyleCostanza Jucker; Augusto Loni; Mattia Calzolari; Sergey Belokobylskij; Daniela Lupi. 2018. "ACCIDENTAL INTRODUCTION IN ITALY OF THE PARASITOID SPATHIUS VULNIFICUS WILKINSON (HYMENOPTERA BRACONIDAE DORYCTINAE)." Redia , no. : 189-191.
Plants have developed a plethora of signals to interact with other organisms, finally building up a sophisticate language for communication. In this context, we investigated Salvia verticillata L. (Lamiaceae), with the primary goal to link secondary metabolites and actual biotic relationships. We specifically analysed the volatile organic compounds (VOC) spontaneously emitted by leaves and flowers and determined the composition of the essential oils obtained from the aerial parts across 2015 and 2016. We merged information of chemical analyses to a micromorphological investigation on the glandular indumentum and to focal observations on the pollinator assemblage. The VOC profiles were highly variable, with the floral bouquet being the most complex. Flowers and leaves showed 37 and 20 exclusive compounds, dominated by 1,8-cineole (10.4%) and germacrene D (38.4%), respectively. Sesquiterpene hydrocarbons prevailed (83.3% leaves; 73.7% flowers) and 19 common compounds were detected. The oil profiles proved to be consistent across the two years: sesquiterpene hydrocarbons invariably dominated, with germacrene D, bicyclogermacrene and β-caryophyllene as main compounds. The whole plant epidermis is thickly covered by two types of glandular hairs: peltates and small capitates, both responsible for the synthesis of terpenes, finally resulting in the VOC emission and in the essential oil production. S. verticillata attracted mainly bees belonging to two functional groups: medium-sized and large bees, notwithstanding the small size of its flowers. At the site, Apis mellifera and different Bombus species were recorded, mainly interested in feeding on nectar. The literature survey on the isolated volatile compounds confirmed the hypotheses on the seduction strategies towards Apoidea.
Claudia Giuliani; Roberta Ascrizzi; Daniela Lupi; Giacomo Tassera; Laura Santagostini; Manuela Giovanetti; Guido Flamini; Gelsomina Fico. Salvia verticillata: Linking glandular trichomes, volatiles and pollinators. Phytochemistry 2018, 155, 53 -60.
AMA StyleClaudia Giuliani, Roberta Ascrizzi, Daniela Lupi, Giacomo Tassera, Laura Santagostini, Manuela Giovanetti, Guido Flamini, Gelsomina Fico. Salvia verticillata: Linking glandular trichomes, volatiles and pollinators. Phytochemistry. 2018; 155 ():53-60.
Chicago/Turabian StyleClaudia Giuliani; Roberta Ascrizzi; Daniela Lupi; Giacomo Tassera; Laura Santagostini; Manuela Giovanetti; Guido Flamini; Gelsomina Fico. 2018. "Salvia verticillata: Linking glandular trichomes, volatiles and pollinators." Phytochemistry 155, no. : 53-60.
The Asian chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu, is one of the most serious pests of Castanea spp. worldwide. Since local parasitoids did not effectively control this exotic pest, the Chinese parasitoid Torymus sinensis Kamijo was released in Japan and Europe resulting in a successful control of the invader. A survey of native parasitoids usually infesting oak gall wasps but associated with D. kuriphilus was carried out, sampling chestnut galls in Italy in 2013. The effect of predictive variables (years since T. sinensis release, oak occurrence, type of chestnut formation, latitude) on composition and density of native parasitoid populations was modelled with Generalized Linear Models. In total, 4,410 chalcid parasitoids were recorded, and the most abundant species were T. flavipes Walker, Eupelmus spp., and Bootanomyia (= Megastigmus) dorsalis (F.). Introduction of T. sinensis and oak occurrence influenced native parasitoid richness, conversely to the latitude of the study area. In particular, every additional year of presence of T. sinensis was proved to determine the loss of about 14% of native species, and 32% of their population density. A positive correlation between the oak density occurring in the infested chestnut forests and the richness and abundance of native parasitoid species was recorded as well, highlighting as generalist parasitoids may switch hosts easily. A positive effect of increasing densities of oak trees was recorded on most of the taxa, except for T. geranii (Walker), Eupelmus spp., and Eurytoma pistaciae Rondani. Our results provide insight that both T. sinensis and oak trees affect native parasitoids, shedding light on the possible implications related to the release of exotic biological control agents on the recruitment and accumulation of native natural enemies.
Chiara Ferracini; Sandro Bertolino; Umberto Bernardo; Carmelo P. Bonsignore; Massimo Faccoli; Ester Ferrari; Daniela Lupi; Stefano Maini; Luca Mazzon; Francesco Nugnes; Anna Rocco; Fabrizio Santi; Luciana Tavella. Do Torymus sinensis (Hymenoptera: Torymidae) and agroforestry system affect native parasitoids associated with the Asian chestnut gall wasp? Biological Control 2018, 121, 36 -43.
AMA StyleChiara Ferracini, Sandro Bertolino, Umberto Bernardo, Carmelo P. Bonsignore, Massimo Faccoli, Ester Ferrari, Daniela Lupi, Stefano Maini, Luca Mazzon, Francesco Nugnes, Anna Rocco, Fabrizio Santi, Luciana Tavella. Do Torymus sinensis (Hymenoptera: Torymidae) and agroforestry system affect native parasitoids associated with the Asian chestnut gall wasp? Biological Control. 2018; 121 ():36-43.
Chicago/Turabian StyleChiara Ferracini; Sandro Bertolino; Umberto Bernardo; Carmelo P. Bonsignore; Massimo Faccoli; Ester Ferrari; Daniela Lupi; Stefano Maini; Luca Mazzon; Francesco Nugnes; Anna Rocco; Fabrizio Santi; Luciana Tavella. 2018. "Do Torymus sinensis (Hymenoptera: Torymidae) and agroforestry system affect native parasitoids associated with the Asian chestnut gall wasp?" Biological Control 121, no. : 36-43.
Marco Valerio Rossi Stacconi; Nasim Amiresmaeili; Antonio Biondi; Cristiano Carli; Stefano Caruso; Maria Luisa Dindo; Santolo Francati; Angela Gottardello; Alberto Grassi; Daniela Lupi; Elisa Marchetti; Fabio Mazzetto; Nicola Mori; Tommaso Pantezzi; Luciana Tavella; Giovanna Tropea Garzia; Lorenzo Tonina; Giacomo Vaccari; Gianfranco Anfora; Claudio Ioriatti. Host location and dispersal ability of the cosmopolitan parasitoid Trichopria drosophilae released to control the invasive spotted wing Drosophila. Biological Control 2018, 117, 188 -196.
AMA StyleMarco Valerio Rossi Stacconi, Nasim Amiresmaeili, Antonio Biondi, Cristiano Carli, Stefano Caruso, Maria Luisa Dindo, Santolo Francati, Angela Gottardello, Alberto Grassi, Daniela Lupi, Elisa Marchetti, Fabio Mazzetto, Nicola Mori, Tommaso Pantezzi, Luciana Tavella, Giovanna Tropea Garzia, Lorenzo Tonina, Giacomo Vaccari, Gianfranco Anfora, Claudio Ioriatti. Host location and dispersal ability of the cosmopolitan parasitoid Trichopria drosophilae released to control the invasive spotted wing Drosophila. Biological Control. 2018; 117 ():188-196.
Chicago/Turabian StyleMarco Valerio Rossi Stacconi; Nasim Amiresmaeili; Antonio Biondi; Cristiano Carli; Stefano Caruso; Maria Luisa Dindo; Santolo Francati; Angela Gottardello; Alberto Grassi; Daniela Lupi; Elisa Marchetti; Fabio Mazzetto; Nicola Mori; Tommaso Pantezzi; Luciana Tavella; Giovanna Tropea Garzia; Lorenzo Tonina; Giacomo Vaccari; Gianfranco Anfora; Claudio Ioriatti. 2018. "Host location and dispersal ability of the cosmopolitan parasitoid Trichopria drosophilae released to control the invasive spotted wing Drosophila." Biological Control 117, no. : 188-196.
Hermetia illucens (L.) (Diptera: Stratiomyidae) is able to consume a wide range of organic matter and is of particular interest for waste management. The nutritional value of preimaginal stages, in particular the protein content, makes this species a valid candidate for use as feed for other organisms. Vegetables and fruits are promising rearing substrates for insects produced for this purpose according to the EU regulation. In order to examine the effects of diets on insect performance and chemical composition, larvae were reared on the following substrates: 1) fruit (apple, pear, and orange); 2) vegetable (lettuce, green beans, and cabbage); and 3) mixed fruits and vegetables. High percentages of survival were observed on all diets, but there were differences among weights of larvae, pupae, and adults, with weights of larvae reared on mixed fruits and vegetables lower than on other diets. Pupae reared on the mixed diet were heaviest, and also morphometric measurements of adults were highest. Larvae reared on fruit diets had the highest fat content, comprising mostly saturated fatty acids; the highest content of essential n-3 fatty acids was found in vegetable reared larvae and that of n-6 in mixed reared larvae. Larvae reared on the mixed diet had the highest protein content. Calcium contents were high and moderate amounts of iron and zinc were found. H. illucens showed the capability to develop on vegetable and fruits diets displaying different nutrient profiles and biological performances. The best-performing rearing strategy should vary in relation to the final use of H. illucens.
Costanza Jucker; Daniela Erba; Maria Giovanna Leonardi; Daniela Lupi; Sara Savoldelli. Assessment of Vegetable and Fruit Substrates as Potential Rearing Media for Hermetia illucens (Diptera: Stratiomyidae) Larvae. Environmental Entomology 2017, 46, 1415 -1423.
AMA StyleCostanza Jucker, Daniela Erba, Maria Giovanna Leonardi, Daniela Lupi, Sara Savoldelli. Assessment of Vegetable and Fruit Substrates as Potential Rearing Media for Hermetia illucens (Diptera: Stratiomyidae) Larvae. Environmental Entomology. 2017; 46 (6):1415-1423.
Chicago/Turabian StyleCostanza Jucker; Daniela Erba; Maria Giovanna Leonardi; Daniela Lupi; Sara Savoldelli. 2017. "Assessment of Vegetable and Fruit Substrates as Potential Rearing Media for Hermetia illucens (Diptera: Stratiomyidae) Larvae." Environmental Entomology 46, no. 6: 1415-1423.
The brown marmorated stink bug, Halyomorpha halys (Stål) is a pest of numerous annual and perennial crops. Additional distribution records for H. halys are provided from northern Italy where rice is cultivated, and the presence of adults feeding on panicles gives the first evidence of an association between this pest and rice (Oryza sativa L.), a crop not previously recorded as a host plant.
Daniela Lupi; Paride Dioli; L. Limonta. First evidence of Halyomorpha halys (Stål) (Hemiptera Heteroptera, Pentatomidae) feeding on rice (Oryza sativa L.). Journal of Entomological and Acarological Research 2017, 49, 1 .
AMA StyleDaniela Lupi, Paride Dioli, L. Limonta. First evidence of Halyomorpha halys (Stål) (Hemiptera Heteroptera, Pentatomidae) feeding on rice (Oryza sativa L.). Journal of Entomological and Acarological Research. 2017; 49 (1):1.
Chicago/Turabian StyleDaniela Lupi; Paride Dioli; L. Limonta. 2017. "First evidence of Halyomorpha halys (Stål) (Hemiptera Heteroptera, Pentatomidae) feeding on rice (Oryza sativa L.)." Journal of Entomological and Acarological Research 49, no. 1: 1.