One of the most vital environmental factors that restricts plant production in arid and semi-arid environments is the lack of fresh water and drought stress. Common bean (Phaseolus vulgaris L.) productivity is severely limited by abiotic stress, especially climate-related constraints. Therefore, a field experiment in split-plot design was carried out to examine the potential function of ascorbic acid (AsA) in mitigating the adverse effects of water stress on common bean. The experiment included two irrigation regimes (100% or 50% of crop evapotranspiration) and three AsA doses (0, 200, or 400 mg L−1 AsA). The results revealed that water stress reduced common bean photosynthetic pigments (chlorophyll and carotenoids), carbonic anhydrase activity, antioxidant activities (2,2-diphenyl-1-picrylhydrazyl free radical activity scavenging activity and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay), growth and seed yield, while increased enzymatic antioxidants (peroxidase), secondary metabolites (phenolic, flavonoids, and tannins), malondialdehyde (MDA), and crop water productivity. In contrast, the AsA foliar spray enhanced all studied traits and the enhancement was gradual with the increasing AsA dose. The linear regression model predicted that when the AsA dose increase by 1.0 mg L−1, the seed yield is expected to increase by 0.06 g m−2. Enhanced water stress tolerance through adequate ascorbic acid application is a promising strategy to increase the tolerance and productivity of common bean under water stress. Moreover, the response of common bean to water deficit appears to be dependent on AsA dose.
1 June 2012 in Journal of the Saudi Society of Agricultural Sciences
A field experiment was carried out to study the effect of organic and bioorganic fertilizers on growth parameters, yield and the quality of two broccoli cultivars (Calabrese and Southern star). Bio-organic fertilizers gave better results for all vegetative growth parameters for Southern star cultivar compared to Calabrese cultivar. Total phenolic content (TPC), total flavonoid content (TFC) and total glucosinolates content (TGsC) were almost higher in Calabrese cultivar as a result of organic fertilizer treatment. The antioxidant activities of both cultivars were evaluated and Calabrese cultivar showed the higher1, 1-diphenyl-2-picrylhydrazyl DPPH. scavenging activity expressed as IC50 (its concentrations for 50% inhibition) (16.56μg/ml) compared to Southern star (19.42μg/ml). In addition, Calabrese cultivar showed the higher chelating power (75.36μg/ml) than Southern star (72.43μg/ml) at (30μg/ml) when the organic fertilizer was applied. The results indicated that there is a good margin for enhancing antioxidant compounds of broccoli for economic production using organic fertilization. This study indicated the potential application of broccoli as a potent natural source of antioxidants as nutraceuticals
1 January 2015 in Journal of the Saudi Society of Agricultural Sciences
Field experiment was conducted to study the effect of organic and bio-organic fertilizers on dry weight; yield, total phenolics (TPC), total flavonoids (TFC), vitamin C and on their antioxidant activities of two sweet fennel cultivars Dolce and Zefa fino. Results strongly showed that there were significant differences between sweet fennel cultivars. Generally the highest values of all parameters were obtained when fennel plants were supplemented with 50% NPK+50% organic fertilizer and bio fertilizer when compared with control treatment. The highest values of TPC, TFC and Vit. C were recorded by Zefa fino cultivar when received 50% NPK+50% organic treatment. The antioxidant activities of both cultivars were evaluated and Dolce cultivar showed the highest DPPH scavenging activity expressed as IC50 compared with Zefa fino cultivar. In addition, Dolce cultivars exhibited the highest value for Fe2+-chelating activities for organic and bio-organic fertilizers followed by Zefa fino when compared to control treatment. Dolce cultivar generally showed superiority than Zefa fino in all measured parameters
Thirty-two male Wistar albino rats were chosen to test the possible protective role of antioxidants of the edible seaweed Sargassum vulgare as a functional food additive to alleviate oxidative stress and toxicity associated with consumption of the artificial sweetener ‘aspartame (ASP)’. Biochemical and spleen histopathological analyses of the orally ASP-administrated rats, at a dose of 500 mg/kg for one week daily, showed different apoptotic and inflammatory patterns. Rats treated with ASP and then supplemented orally with the S. vulgare-MeOH extract, at a dose of 150 mg/kg for three consecutive weeks daily, showed significant positive reactions in all investigated assays related to ASP consumption. The protective and immune-stimulant efficacy of S. vulgare-MeOH extract, inferred from combating oxidative stress-induced lipid peroxidation, modulating the low levels of the endogenous antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and of the thyroid hormones T3 and T4, attenuating the elevated levels of apoptotic CASP-3 and inflammatory biomarkers TNF-α and IL-6, as well as heat shock proteins (Hsp70), can be most likely ascribed to the synergistic effect of its potent antioxidant phenolics (mainly gallic, ferulic, salicylic, and chlorogenic, and p-coumaric acids) and flavonoids (rutin, kaempferol, and hesperidin). Mechanism of action of these natural antioxidants was discussed.