This review article summarises and discusses the recent developments in different biomedical applications of electrospun chitosan nanofibers.

ESC (Escitalopram) and ETZ (Etizolam) are essential anti-anxiety medications. This research suggests employing a Green Analytical Quality by Design (AQbD) based HPLC technique to determine ESC and ETZ are environmentally friendly. According to literature, there is currently no published article by this method. Using Phenomenex column C₁₈ containing ethanol and phosphate buffer (60:40 percent v/v) and the flow rate is fixed to be 1 mL/ min, and the detection was carried out at 254 nm the proposed approach can separate ESC and ETZ and their degradation products. Retention times of ESC and ETZ were found to be 3.5 and 6.5 min respectively. This approach was verified and demonstrated linearity ranges from 5–30 μg/mL for ESC, 2–12 μg/mL for ETZ, accuracy, repeatability, and selectivity, with R² values were 0.9975 and 0.9987 for ESC and ETZ, respectively. The novel method was also evaluated using four different approaches,including other assessment methods: NEMI, GAPI, AGMS, AGREE and it was found to been environment friendly. Based on our results, HPLC techniques with the Green Analytical Performances by the QbD technique for evaluating stability of the drug might be benefit in the development of novel pharmaceutical drugs such as ESC and ETZ. GRAPHICAL ABSTRACT

Wound healing is an unresolved therapeutic challenge for the medical community, as wound assessment and management is a complex procedure involving numerous factors that play a significant role in the healing process. Several factors, including bacterial infections, underlying conditions, malnutrition, obesity, aging, and smoking are the most frequent causes of a delayed wound-healing process. The shortcomings related to the currently used wound dressings include poor antimicrobial properties, weak mechanical features, poor biodegradability, biocompatibility, etc. Modern medicine has expanded the use of phytoconstituents based on nanotechnology to regenerate and repair soft and hard tissues. Electrospun nanofiber platforms are the most recent and promising among many types of conventional wound dressings due to their distinct characteristics. Many plant extracts and their phytoconstituents are well-known as adequate substitutes for wound healing agents because of their wide range of active ingredients, accessibility, and limited side effects. Incorporating these phytoconstituents into electrospun nanofibers combines the structural properties of the nanofibers with the antibacterial and therapeutic properties of the plants, making the nanofibers ideal for use as wound dressings. This review focuses on the antibacterial and therapeutic applications of nanofiber wound dressings containing phytoconstituents and their potential to revolutionize wound healing.