The occurrence of adventitious roots and somatic embryos is a crucial step in micropropagation that frequently limits the application of this technique in woody plants. Recent studies demonstrated that they can be negatively or positively regulated with γ-aminobutyric acid (GABA), which is a four-carbon non-proteinous amino acid that not only acts as a main inhibitory neurotransmitter in mammals. It has been reported that GABA affects plant growth and their response to stress although its mode of action is still unclear. This review dealt with the effects of GABA on adventitious root formation and growth as well as on somatic embryogenesis. Furthermore, we focused on discussing the interaction of GABA with phytohormones, such as auxin, ethylene, abscisic acid, and gibberellin, as well as with the carbon and nitrogen metabolism during adventitious root development. We suggested that research on GABA will contribute to the application of micropropagation in the recalcitrant fruit and forest species.

Populus deltoides holds significant ecological and economic importance and is a crucial gene donor for the world’s staple poplar varieties. To select and breed P. deltoides with improved agronomic traits, nine growth and leaf traits were examined in 375 different genotypes, assessing their genetic diversity and performing correlation and comprehensive ranking analyses. Phenotyping results were then utilized to screen a total of 2,009,263 SNP (single nucleotide polymorphism) loci significantly associated with the nine phenotypic traits. A total of 45 SNP loci exhibited significant associations with growth traits based on a general linear model (GLM) analysis. By analyzing the Linkage disequilibrium (LD) block of five SNP loci with significant leaf area and height, we identified five candidate genes related to leaf area and height. Three of the five SNP loci were successfully validated using KASP (kompetitive allele-specific PCR) assays. One loci Chr08_16007979 was closely linked with leaf area, and two loci Chr05_12148738, and Chr05_17106547 were closely linked with height. The developed functional KASP markers offer valuable insights for subsequent further marker-assisted breeding and genetic improvement studies in southern-type poplars.

Chinese chestnut (Castanea mollissima) is a multipurpose tree providing nuts and timbers, which holds an important position in the mountainous villages in China. However, leaf scorch disease is becoming more and more serious in the chestnut orchards of Yanshan Mountain areas, but the cause of occurrence is still unclear. In this study, the nutrient elements were analyzed from the leaves, roots, and surrounding soils of roots as well as the nut qualities in the healthy and scorched trees from two adjacent chestnut orchards. The results showed that the elements of nitrogen (N), iron (Fe), boron (B), and zinc (Zn) in leaves significantly increased in the scorched trees as well as N and B in roots, and potassium (K), and available potassium (AP) in soils, but leaf magnesium (Mg), root manganese (Mn), and soil Mg, copper (Cu), Fe, and B significantly decreased. Correlation analysis demonstrated that B, Zn, Mg, and Fe had a greater influence on the status of leaf health, and soil AK, K, Fe, B, and Cu had an impact on leaf B concentration. In addition, the occurrence of leaf scorch affected the nut sizes, contents of total soluble proteins and ascorbic acid as well as the catalase activity in the nuts. These results indicated that the disruption of soil-element balance may be one of the main causes resulting in the occurrence of leaf scorch, which would provide a theoretical basis and practical guidance for the prevention of chestnut leaf scorch disease.