In recent years, wearable sensors have enabled the unique mode of real-time and noninvasive monitoring to develop rapidly in medical care, sports, and other fields. Sweat contains a wide range of biomarkers such as metabolites, electrolytes, and various hormones. Combined with wearable technology, sweat can reflect human fatigue, disease, mental stress, dehydration, and so on. This paper comprehensively describes the analysis of sweat components such as glucose, lactic acid, electrolytes, pH, cortisol, vitamins, ethanol, and drugs by wearable sensing technology, and the application of sweat wearable devices in glasses, patches, fabrics, tattoos, and paper. The development trend of sweat wearable devices is prospected. It is believed that if the sweat collection, air permeability, biocompatibility, sensing array construction, continuous monitoring, self-healing technology, power consumption, real-time data transmission, specific recognition, and other problems of the wearable sweat sensor are solved, we can provide the wearer with important information about their health level in the true sense.

Abnormal protein phosphorylation may relate to diseases such as Alzheimer’s, schizophrenia, and Parkinson’s. Therefore, the real-time detection of phosphoproteins in sweat was of great significance for the early knowledge, detection, and treatment of neurological diseases. In this work, anatase/rutile TiO₂ was in situ grown on the MXene surface to constructing the intercalation structure MXene@anatase/rutile TiO₂ ternary heterostructure as a sensing platform for detecting phosphoprotein in sweat. Here, the intercalation structure of MXene acted as electron and diffusion channels for phosphoproteins. The in situ grown anatase/rutile TiO₂ with n-n-type heterostructure provided specific adsorption sites for the phosphoproteins. The determination of phosphoprotein covered concentrations in sweat, with linear range from 0.01 to 1 mg/mL, along with a low LOD of 1.52 μM. It is worth noting that, since the macromolecular phosphoprotein was adsorbed on the surface of the material, the electrochemical signal gradually decreased with the increase of phosphoprotein concentration. In addition, the active sites in the MXene@anatase/rutile TiO₂ ternary heterojunction and synergistic effect of the heterojunction were verified by first-principle calculations to further realize the response to phosphoproteins. Additionally, the effective diffusion capacity and mobility of phosphoprotein molecules in the ternary heterojunction structure were studied by molecular dynamics simulation. Furthermore, the constructed sensing platform showed high selectivity, repeatability, reproducibility, and stability, and this newly developed sensor can detect for phosphoprotein in actual sweat samples. This satisfactory sensing strategy could be promoted to realize the noninvasive and continuous detection of sweat.

Abnormal protein phosphorylation in sweat metabolites is closely related to cancer, cardiovascular disease, and other diseases. The real-time monitoring of phosphoproteins in sweat is significant for early monitoring of disease biomarkers. Here, a high-efficiency electrochemical sensor for phosphoprotein in sweat was realized by 2D@3D g-C₃N₄@Fe₃O₄ with intercalation structure. Common phosphoprotein β-Casein was selected to demonstrate the platform’s functionalities. The detection limit of g-C₃N₄@Fe₃O₄ could be as low as 9.7 μM, and the detection range was from 0.01 mg/mL to 1 mg/mL. In addition, the sensing platform showed good selectivity, reproducibility, and stability. We also investigated the effects of interface structure on adsorption properties and electronic properties of the g-C₃N₄ and Fe₃O₄ heterostructure using DFT. More electrons from Fe₃O₄ were transferred to g-C₃N₄, which increased the electrons in the energy band of N atoms and promoted the formation of stable N-H bonds with H atoms in phosphoproteins. We demonstrated phosphoprotein sensor functionality by measuring the phosphoprotein in human sweat during exercising. This work realizes a sensing platform for noninvasive and continuous detection of sweat phosphoproteins in wearable devices.

Introduction: Ectoine, a natural protective agent produced by halophilic bacteria, demonstrates remarkable skin-protective efficacy; however, the precise molecular mechanisms underlying its effects on skin cells remain poorly understood. This study investigated the differentially expressed genes (DEGs) in human keratinocytes (HaCaT) treated with ectoine to clarify its protective mechanisms. Methods: Cell viability was evaluated using the CCK-8 assay, identifying 0.2 mg/mL as the optimal ectoine concentration. A control group (C, 0 mg/mL) and an ectoine group (E, 0.2 mg/mL) were established. Flow cytometry quantified reactive oxygen species (ROS) and apoptosis. Illumina HiSeq RNA-seq identified DEGs; selected genes were validated by RT-qPCR. Results: Ectoine at 0.2 mg/mL increased cell viability to 134.0 % ± 3.2. Compared with the control group, ectoine significantly reduced the apoptosis rate and intracellular ROS levels (P ≤ 0.05). RNA-seq (n = 3) identified 292 DEGs (87 up-regulated, 205 down-regulated). Among them, MMP25, NOXO1, ANGPTL4, and FoXO6—genes involved in oxidative stress, apoptosis, and proliferation—were markedly down-regulated, suggesting enhanced proliferation and anti-oxidative, anti-apoptotic effects. The cell-adhesion gene CNFN was significantly up-regulated, potentially reducing mechanical damage. Conclusion: Ectoine serves as a potent stabilizer and protectant, safeguarding skin by modulating genes that regulate oxidative stress, proliferation, and apoptosis.