Important role of alfalfa in development of animal husbandry, is based on high potential for production of biomass. Reaching of high green (over 80 t ha-1) and dry matter yield (approx. 20 t ha-1) with low investment during all period of utilization makes alfalfa production very economical. Divergent selection materials were used to create high productive domestic alfalfa cultivars with excellent field persistence, which is important factor for high and stable yield during entire exploitation period. On the other hand, alfalfa is a rich source of crude protein with excellent digestibility. Proteins from alfalfa herbage are the cheapest source of protein in animal feed. Content of crude protein strongly depend on development stage of plant material and can be 200 to 240 g kg-1 DM, while crude protein yield can be over 3.5 t ha-1. Besides that, alfalfa herbage had a high content of macro and micro element, which are so important for animal health. It is usually grown in pure stand, although it's a good component of grass-legumes mixtures. Alfalfa biomass can be used in several ways, usually as hay, but also as high quality silages, haylages, dehydrated in form of briquettes or by grazing. Symbiotic N2 fixation gives alfalfa not just economical, but very high ecological importance, too. It's a reason why alfalfa, besides important role in conventional husbandry is getting great role in sustainable agriculture and organic production. All those characteristics confirm, that alfalfa is a really 'queen' of forages.

Content of crude protein and cell wall constituents in leaves and stems of alfalfa (Medicago sativa L.) cv K-22 with advancing maturity was investigated. Sampling was done in 7-day intervals in second cut. During growth and development, crude protein content decreased in leaves and stems, from 332,6 to 328,8 gkg-1DM and from 138,6 to 122,0 gkg-1DM, respectively. From first to third development stage, content of ADF in leaves and stems increased, from 352,0 to 476,7 gkg-1DM and from 592,8 to 867,1 gkg-1DM, respectively. Highest contents of ADF and hemicellulose was stated in plant parts in third development stage, in leaves 187,4 and 289,3 gkg-1DM, and in stems 499,4 and 367,7 gkg-1DM. The highest changes of lignin contents are in stems, while content of lignin in leaves increased from 42,0 to 49,0 gkg-1DM.

Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC₅₀=0.39 µg mL⁻¹) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC₅₀=0.61 µg mL⁻¹), followed by azoxystrobin (mean EC₅₀=2.83 µg mL⁻¹) and flutriafol (mean EC₅₀=2.11 µg mL⁻¹). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC₅₀=35.31 and 62.83 µg mL⁻¹, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC₅₀=0.49 and 0.19 µg mL⁻¹, respectively), while the rest of isolates were highly resistant.

Efficient germination of fescue seeds is essential for successful establishment of meadows and pastures. This research was conducted to ascertain the effects of various acid and temperature treatments on seed germination in three fescue species: Festuca rubra, F. ovina, and F. pratensis. Seeds from different cultivars, populations, or lots were exposed either to four concentrations of sulfuric acid at three different time intervals (12 treatments) or six different temperatures at three different time intervals (18 treatments). Despite all belonging to the genus Festuca, the seed from different species responded differently to the treatments. The three optimum treatments for F. rubra seed involved soaking in a 75% solution of sulfuric acid for 20 minutes (improved the germination rate by 19%), soaking in a 50% solution of sulfuric acid for 30 minutes (improved the germination rate by 18%) and exposure to either 60°C or 70°C for 90 minutes (improved the germination rate by 17%). For F. ovina seed, optimal treatments included soaking seeds for either 10 or 20 minutes in a 50% sulfuric acid solution (both treatments improved germination rates by 13%) or exposing seeds for 30 minutes in a 25% sulfuric acid solution and 80°C for 60 minutes (improved germination rate by 12%). Two optimal treatments were identified for F. pratensis seed. Whereas the first involved soaking the seeds in a 75% sulfuric acid solution for 30 minutes (improved germination rates by 22%), the second involved either exposing the seeds to 90°C for 90 or 60 minutes, or exposing the seeds to 80°C for 90 minutes (improved germination rate by 21%). Our findings indicate that if fescue seed is to be sown during the autumn (two to three months after seed collecting), treating it with acid and temperature can significantly enhance its germination.