We present a novel technique for considerably decreasing the sidelobe height of the axial point-spread function of one-photon 4Pi-confocal microscopes. By means of a numerical example, in which the ratio between the excitation and the fluorescence wavelengths was set to $\mathit{ϵ}={\mathrm{\lambda }}_{\mathrm{exc}}/{\mathrm{\lambda }}_{\det }=0.8,$ we show that simply inserting a pair of properly designed two-ring binary masks in the illumination set allows the height of the axial sidelobes to be reduced from 20% to 5% of the height of the central peak. This allows one to receive the full benefit of the strong narrowness of the central lobe provided by the 4Pi-confocal technique.

To evaluate the intrasession repeatability of anterior chamber depth (ACD), central (CCT) and peripheral corneal thickness (PCT), white-to-white diameter (WTW), and irido-corneal angle (IA) measurements obtained with a multidiagnostic device in healthy eyes. A total of 107 eyes of 107 patients ranging in age from 23 to 65 years were examined with the VX120 system (Visionix-Luneau Technologies). Three consecutive measurements were obtained with this device to assess the intrasession repeatability of ACD, CCT, PCT at different nasal and temporal locations, WTW, and nasal and temporal IA. Data analysis included the calculation of within-subject standard deviation (S_w), intrasubject precision (1.96xS_w), coefficient of variation (CV) and intraclass correlation coefficient (ICC). The S_w and CV for ACD was 0.03 mm and 1.16%, respectively, with an ICC of 0.992. The S_w values for central and peripheral pachymetric measurements were below 9 μm, with CV of less than 1.6% and ICC of 0.976 or higher. For IA measurements, S_w values of 0.84 or lower were found, with a CV between 1 and 2%, and an ICC of more than 0.970. The S_w for WTW was 0.24 mm and the CV was 1.95%. No statistically significant correlations were found between any anatomical parameter evaluated and their S_w and CV values associated (−0.220 ≤ r ≤ 0.204, p ≥ 0.125). The VX120 system is able to provide repeatable measurements of anatomical parameters in healthy eyes. Inter-observer repeatability should be evaluated in future studies.

In this work we try to understand the real effect of increase in aberrations after Femto-LASIK surgery on the patient’s final visual quality, specifically when the visual acuity measurement is considered. A clinical study with 37 eyes of 20 patients that underwent myopic Femto-LASIK surgery and different personalized eye model simulations were carried out. In clinical study, correlations between pre- and postoperative parameters with visual acuity were analysed. Eye simulations (based on real data) provided simulations of vision quality before and after surgery. Our main results showed a significant increase in aberrations was obtained after surgery; however, no differences were found between the preoperative corrected distance visual acuity (CDVA) and the postoperative uncorrected distance visual acuity (UDVA). This absence of differences in visual quality could be explained by performing different simulations on three eyes that would cover most of the possible clinical situations. Simulations were implemented considering a pupil size of 2.5 mm and the personalized data of each patient. Results showed that final visual acuity (VA) change are determined by the final high-order aberrations (HOAS) and their increase after surgery but measured under photopic conditions. In conclusion, customized analysis of higher-order aberrations in scotopic pupils better predicts patient visual acuity after Lasik surgery.

Background/Objectives: To assess the optical performance of two refractive premium IOLs across pupil sizes and values of corneal spherical aberration (SA). Methods: Two refractive IOLs were evaluated in this study: Tecnis Eyhance and Mini Well. The surface profiles were obtained to calculate the through-object MTF (TO MTF) curves and simulate optotype images. Entrance pupil sizes ranging from 2 to 5.5 and three corneal models were analyzed in the simulation: an average population aberrated cornea, an aberration-free cornea and a post-Lasik myopic cornea. Results: For Model 1 and pupil sizes between 3.0 and 3.5 mm, Mini Well provided acceptable visual quality from far to near distances, whereas Eyhance struggled to maintain visual quality at distances closer than intermediate. For patients with lower-than-normal corneal SA (i.e., more prolate corneas, such as post-hyperopic LASIK) both IOLs exhibited a hyperopic shift in far focus. Conversely, for patients with higher-than-normal corneal SA (i.e., more oblate corneas, such as post-myopic LASIK), the shift occurred in the myopic direction. Despite the implementation of an optimized IOL power to circumvent any shift, the TO MTF nevertheless reflected the interaction between corneal and IOL SA. Furthermore, the Mini Well demonstrated increased tolerance to less negative SA values, while Eyhance exhibited behavior consistent with a monofocal lens for more positive SA values. Conclusions: Surgeons should consider each patient’s corneal asphericity and typical pupil diameter when selecting and calculating the power of the premium IOLs studied, particularly in patients with a history of refractive surgery.

We present a simple experiment, specifically designed for students of undergraduate optics courses, where the influence of an aperture stop position on the three-dimensional structure of the focal volume of focusing systems is studied. The experiment, which involves only simple optical elements, permits an undergraduate student to generate different focal structures by simply axially displacing the aperture stop.