Spherical Janus particles are one of the most prominent examples for active Brownian objects. Here, we study the diffusiophoretic motion of such microswimmers in experiment and in theory. Three stages are found: simple Brownian motion at short times, superdiffusion at intermediate times, and finally diffusive behavior again at long times. These three regimes observed in the experiments are compared with a theoretical model for the Langevin dynamics of self-propelled particles with coupled translational and rotational motion. Besides the mean square displacement also higher displacement moments are addressed. In particular, theoretical predictions regarding the non-Gaussian behavior of self-propelled particles are verified in the experiments. Furthermore, the full displacement probability distribution is analyzed, where in agreement with Brownian dynamics simulations either an extremely broadened peak or a pronounced double-peak structure is found, depending on the experimental conditions.

Confined diffusion is ubiquitous in nature. Ever since the “anomalous yet Brownian” motion was observed, the non-Gaussianity in confined diffusion has been unveiled as an important issue. In this Letter, we experimentally investigate the characteristics and source of non-Gaussian behavior in confined diffusion of nanoparticles suspended in polymer solutions. A time-varied and size-dependent non-Gaussianity is reported based on the non-Gaussian parameter and displacement probability distribution, especially when the nanoparticle’s size is smaller than the typical polymer mesh size. This non-Gaussianity does not vanish even at the long-time Brownian stage. By inspecting the displacement autocorrelation, we observe that the nanoparticle–structure interaction, indicated by the anticorrelation, is limited in the short-time stage and makes little contribution to the non-Gaussianity in the long-time stage. The main source of the non-Gaussianity can therefore be attributed to hopping diffusion that results in an exponential probability distribution with the large displacements, which may also explain certain processes dominated by rare events in the biological environment.

We report the cross-interface emulsification (XiE), a simple method for generation of monodisperse droplets of controllable volumes from picoliter to nanoliter. A simple system is set up, in which a fused-silica capillary is vibrating across the surface of the continuous phase (mineral oil) in a reservoir, and the flow of the dispersed phase (aqueous solution) in the capillary is segmented into monodisperse droplets at the air-oil interface. We find that the volume of droplets is mainly dominated by the flow rate and vibrating frequency, and not significantly influenced by other factors, such as the viscosity of continuous phase and dispersed phase, inner diameter of the capillary (20 ~ 100 μm), and the shape of the tip (tapered or flat). These features reflect high robustness, flexibility and precision of the XiE method in on demand volume control of droplets. The droplets automatically assembled into planar monolayer droplet arrays (PMDA) in flat-bottomed microwells of 96-well plates, offering excellent convenience for imaging of droplets. As a representative application, we successfully performed digital loop-mediated isothermal amplification using PMDAs with multivolume droplets for the absolute quantification of nucleic acids. Our results demonstrate XiE is simple and inexpensive for the production of monodisperse size-tuneable droplets, and offers opportunities for common laboratories, even without microfabrication facilities, to perform digital quantification, singe cell analysis and other biochemical assays with high throughput.

The double-faced Janus micro-motor, which utilizes the heterogeneity between its two hemispheres to generate self-propulsion, has shown great potential in water cleaning, drug delivery in micro/nanofluidics, and provision of power for a novel micro-robot. In this paper, we focus on the self-propulsion of a platinum–silica (Pt–SiO₂) spherical Janus micro-motor (JM), which is one of the simplest micro-motors, suspended in a hydrogen peroxide solution (H₂O₂). Due to the catalytic decomposition of H₂O₂ on the Pt side, the JM is propelled by the established concentration gradient known as diffusoiphoretic motion. Furthermore, as the JM size increases to O (10 μm), oxygen molecules nucleate on the Pt surface, forming microbubbles. In this case, a fast bubble propulsion is realized by the microbubble cavitation-induced jet flow. We systematically review the results of the above two distinct mechanisms: self-diffusiophoresis and microbubble propulsion. Their typical behaviors are demonstrated, based mainly on experimental observations. The theoretical description and the numerical approach are also introduced. We show that this tiny motor, though it has a very simple structure, relies on sophisticated physical principles and can be used to fulfill many novel functions.