Effie TSAKALIDOU

Dr. Effie TSAKALIDOU

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Research Focus & Metrics

Academic Career Length

27Years (2000-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 December 2002 in Applied and Environmental Microbiology
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Journal Article
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MGMarina D. Georgalaki
Marina D. Georgalaki
EBErika Van Den Berghe
Erika Van Den Berghe
DKDimitrios Kritikos
Dimitrios Kritikos
BDBart Devreese
Bart Devreese
JBJozef Van Beeumen
Jozef Van Beeumen
ETEffie TSAKALIDOU
Effie TSAKALIDOU
Streptococcus macedonicus ACA-DC 198, a strain isolated from Greek Kasseri cheese, produces a food-grade lantibiotic named macedocin. Macedocin has a molecular mass of 2,794.76 ± 0.42 Da, as determined by electrospray mass spectrometry. Partial N-terminal sequence analysis revealed 22 amino acid residues that correspond with the amino acid sequence of the lantibiotics SA-FF22 and SA-M49, both of which were isolated from the pathogen Streptococcus pyogenes . Macedocin inhibits a broad spectrum of lactic acid bacteria, as well as several food spoilage and pathogenic bacteria, including Clostridium tyrobutyricum . It displays a bactericidal effect towards the most sensitive indicator strain, Lactobacillus sakei subsp. sakei LMG 13558 T , while the producer strain itself displays autoinhibition when it is grown under conditions that do not favor bacteriocin production. Macedocin is active at pHs between 4.0 and 9.0, and it retains activity even after incubation for 20 min at 121°C with 1 atm of overpressure. Inhibition of macedocin by proteolytic enzymes is variable.

1 December 2001 in Applied and Environmental Microbiology
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Journal Article
Open access
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PSPanagiotis Sarantinopoulos
Panagiotis Sarantinopoulos
GKGeorge Kalantzopoulos
George Kalantzopoulos
ETEffie TSAKALIDOU
Effie TSAKALIDOU
Citrate metabolism by Enterococcus faecalis FAIR-E 229 was studied in various growth media containing citrate either in the presence of glucose or lactose or as the sole carbon source. In skim milk (130 mM lactose, 8 mM citrate), cometabolism of citrate and lactose was observed from the first stages of the growth phase. Lactose was stoichiometrically converted into lactate, while citrate was converted into acetate, formate, and ethanol. When de Man-Rogosa-Sharpe (MRS) broth containing lactose (28 mM) instead of glucose was used, E. faecalis FAIR-E 229 catabolized only the carbohydrate. Lactate was the major end product, and small amounts of ethanol were also detected. Increasing concentrations of citrate (10, 40, 70, and 100 mM) added to MRS broth enhanced both the maximum growth rate of E. faecalis FAIR-E 229 and glucose catabolism, although citrate itself was not catabolized. Glucose was converted stoichiometrically into lactate, while small amounts of ethanol were produced as well. Finally, when increasing initial concentrations of citrate (10, 40, 70, and 100 mM) were used as the sole carbon sources in MRS broth without glucose, the main end products were acetate and formate. Small amounts of lactate, ethanol, and acetoin were also detected. This work strongly supports the suggestion that enterococcal strains have the metabolic potential to metabolize citrate and therefore to actively contribute to the flavor development of fermented dairy products.

11 January 2022 in Foods
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Journal Article
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RARania Anastasiou
Rania Anastasiou
MKMaria Kazou
Maria Kazou
MGMarina Georgalaki
Marina Georgalaki
AAAnastasios Aktypis
Anastasios Aktypis
GZGeorgia Zoumpopoulou
Georgia Zoumpopoulou
ETEffie TSAKALIDOU
Effie TSAKALIDOU
Cheese is characterized by a rich and complex microbiota that plays a vital role during both production and ripening, contributing significantly to the safety, quality, and sensory characteristics of the final product. In this context, it is vital to explore the microbiota composition and understand its dynamics and evolution during cheese manufacturing and ripening. Application of high-throughput DNA sequencing technologies have facilitated the more accurate identification of the cheese microbiome, detailed study of its potential functionality, and its contribution to the development of specific organoleptic properties. These technologies include amplicon sequencing, whole-metagenome shotgun sequencing, metatranscriptomics, and, most recently, metabolomics. In recent years, however, the application of multiple meta-omics approaches along with data integration analysis, which was enabled by advanced computational and bioinformatics tools, paved the way to better comprehension of the cheese ripening process, revealing significant associations between the cheese microbiota and metabolites, as well as their impact on cheese flavor and quality.

1 October 2006 in International Journal of Food Microbiology
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Journal Article
Closed access
KPKonstantinos Papadimitriou
Konstantinos Papadimitriou
Harris PratsinisHarris Pratsinis
Harris Pratsinis
GNGerhard Nebe-Von-Caron
Gerhard Nebe-Von-Caron
Dimitris KletsasDimitris Kletsas
Dimitris Kletsas
ETEffie TSAKALIDOU
Effie TSAKALIDOU

1 January 2014 in BMC Genomics
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Journal Article
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Konstantinos PapadimitriouKonstantinos Papadimitriou
Konstantinos Papadimitriou
RARania Anastasiou
Rania Anastasiou
EMEleni Mavrogonatou
Eleni Mavrogonatou
JBJochen Blom
Jochen Blom
NPNikos Papandreou
Nikos Papandreou
ETEffie TSAKALIDOU
Effie TSAKALIDOU
Within the genus Streptococcus, only Streptococcus thermophilus is used as a starter culture in food fermentations. Streptococcus macedonicus though, which belongs to the Streptococcus bovis/Streptococcus equinus complex (SBSEC), is also frequently isolated from fermented foods mainly of dairy origin. Members of the SBSEC have been implicated in human endocarditis and colon cancer. Here we compare the genome sequence of the dairy isolate S. macedonicus ACA-DC 198 to the other SBSEC genomes in order to assess in silico its potential adaptation to milk and its pathogenicity status.

1 April 2015 in Current Opinion in Food Science
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Journal Article
Closed access
KPKonstantinos Papadimitriou
Konstantinos Papadimitriou
BPBruno Pot
Bruno Pot
ETEffie TSAKALIDOU
Effie TSAKALIDOU