Alexis Pereira

Mr. Alexis Pereira

Centro de Investigação de Montanha, Bragança, Portugal
orcid

Research Focus & Metrics

Academic Career Length

4Years (2023-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

View All Publications
30 October 2023 in Horticulturae
Verified
Journal Article
Open access
PDF file
VLVasiliki Liava
Vasiliki Liava
CCChristina Chaski
Christina Chaski
Mikel Añibarro-OrtegaMikel Añibarro-Ortega
Mikel Añibarro-Ortega
Alexis PereiraAlexis Pereira
Alexis Pereira
José PinelaJosé Pinela
José Pinela
Spyridon A PetropoulosSpyridon A Petropoulos
Spyridon A Petropoulos
Water shortage can be a restrictive factor for the growth and quality of vegetable crops. Considering the alleviating effects of biostimulant application against water stress, this study aimed to investigate the effect of four biostimulant products (protein and amino acids with carboxylic acids (Tr1); protein and amino acids with seaweed extracts (Tr2); humic and fulvic acids with seaweed extracts (Tr3); SiO2 (Tr4); and control (no biostimulants added)) and two irrigation systems (regulated deficit irrigation (RDI)—65% of field capacity and regular irrigation (RI)—100% of field capacity) on quality parameters of processing tomato fruit. Regulated deficit irrigation and biostimulant application increased the energetic value, carbohydrates, and free sugars content, while organic acids showed a variable response to biostimulant use. In terms of tocopherols (α-, β-, γ-, δ-) and carotenoids (lycopene and β-carotene), regular irrigation and biostimulant application negatively affected their content, while Tr3 treatment had a beneficial impact on these lipophilic compounds under RDI conditions. The main fatty acids were palmitic (C16:0) and linoleic (C18:2n6) acids, which increased when plants were treated with Tr3 and Tr1 biostimulants under a deficit regime. Antioxidant activity (assessed by TBARS and OxHLIA assays) and total phenolic and flavonoids content also showed a variable response to the studied factors. In particular, the application of Tr3 and the control treatment under RDI increased the total phenolic content, while the control and Tr3 treatments under the same irrigation regime recorded the highest antioxidant activity. In conclusion, our results indicate that the adoption of eco-friendly strategies such as regulated deficit irrigation and biostimulant application can beneficially affect the quality traits of processing tomatoes.

23 January 2023 in Antioxidants
Verified
Journal Article
Open access
PDF file
Alexis PereiraAlexis Pereira
Alexis Pereira
Mikel Añibarro-OrtegaMikel Añibarro-Ortega
Mikel Añibarro-Ortega
MKMarina Kostić
Marina Kostić
António NogueiraAntónio Nogueira
António Nogueira
MSMarina Soković
Marina Soković
Lillian BarrosLillian Barros
Lillian Barros
This study aimed to promote the total upcycling of quince (Cydonia oblonga Mill.) peel into bioactive extracts (BEs) and fiber concentrates (FCs). The multicomponent extraction processes were optimized using response surface methodology (RSM) coupled with a 20-run experimental design, where the effects of time (1–120 min), temperature (25–95 °C), and EtOH percentage (0–100%) were combined. In addition to the extraction yields, BEs were analyzed for phenolic compounds, organic acids, and other water-soluble constituents, while FCs were characterized for their color and dietary fiber content. Statistically valid theoretical models were obtained by fitting these dependent variables to a quadratic equation and used to predict optimal extraction conditions. Those obtained for phenolic compounds and malic acid were experimentally validated, yielding 9.3 mg/g and 7.6 g/100 g of these bioactive constituents, respectively, and about 51% (w/w) FC. These BEs showed in vitro antioxidant activity and antimicrobial effects against foodborne fungi and bacteria, standing out in some aspects in relation to synthetic food additives, mainly the malic acid-enriched BE. Overall, the developed extraction processes allowed valorizing of quince peel in FCs and BEs that could be used as natural fortifiers or preservatives in the formulation of foods, beverages and dietary supplements.
1

12 March 2025 in Agronomy
Verified
Journal Article
Open access
PDF file
Mikel Añibarro-OrtegaMikel Añibarro-Ortega
Mikel Añibarro-Ortega
Alexis PereiraAlexis Pereira
Alexis Pereira
José PinelaJosé Pinela
José Pinela
VLVasiliki Liava
Vasiliki Liava
CCChristina Chaski
Christina Chaski
Spyridon A PetropoulosSpyridon A Petropoulos
Spyridon A Petropoulos
This study aimed to evaluate the effect of two irrigation systems (deficit irrigation (DI)—70% of field capacity—and full irrigation (FI)—100% of field capacity) and a biostimulant formulation (silicon (Si) and calcium (Ca) at four different rates) on the chemical composition and fruit quality of greenhouse-grown tomatoes. Deficit irrigation and biostimulant application influenced the proximate composition of tomato fruits. Fructose and glucose were the main soluble sugars, while malic and citric acids were the predominant organic acids. Free sugar and organic acid content increased under DI and biostimulant applications. In contrast, deficit irrigation combined with biostimulant application decreased α-tocopherol levels. In terms of carotenoids, lycopene and β-carotene concentrations were higher under full irrigation. The main fatty acids were palmitic (C16:0) and linoleic (C18:2n6) acids, with saturated (SFA) and polyunsaturated (PUFA) fatty acids being the main classes. Moreover, biostimulant applications reduced the total phenolic content regardless of the irrigation regime, whereas the flavonoid content increased when biostimulants were applied under FI conditions. Regarding antioxidant activity (assessed by TBARS and OxHLIA assays), a variable response to irrigation and biostimulant application was observed. In conclusion, the application of Si and Ca under DI showed promising results in terms of yield and quality of tomato fruit and it could be considered a sustainable strategy to mitigate adverse effects of climate change on horticultural crops.

13 June 2025 in Foods
Verified
Journal Article
Open access
PDF file
Mikel Añibarro-OrtegaMikel Añibarro-Ortega
Mikel Añibarro-Ortega
Maria Inês DiasMaria Inês Dias
Maria Inês Dias
JPJovana Petrovic
Jovana Petrovic
Alexis PereiraAlexis Pereira
Alexis Pereira
MSMarina Soković
Marina Soković
José PinelaJosé Pinela
José Pinela
Lulo or naranjilla (Solanum quitoense Lam.) is an Andean fruit with a sour and refreshing flavor, widely used in the preparation of juices and sweets. Despite its potential for international markets, it remains largely unknown outside its native regions, and most existing studies have focused on the whole fruit or its juice. This study investigated the nutritional and phenolic profiles of the peel, pulp, and seeds of S. quitoense using official food analysis methods and chromatographic techniques. In addition, the in vitro antioxidant activity and antimicrobial effects against foodborne fungi and bacteria were assessed. The peel was rich in ascorbic acid (25.2 mg/100 g fw), α-tocopherol (7.9 mg/100 g fw), dietary fiber (16.5 g/100 g fw), macrominerals (Na, Ca, K), and flavonoids (14.2 mg/g extract); the pulp contained high levels of citric acid (4.22 g/100 g fw) and sucrose (2.7 g/100 g fw); and the seeds stood out for their contents of trace elements (Zn, Cu, Mn, Fe), oleic acid, and spermidine-derived phenolamides (37.8 mg/g extract). Hydroethanolic extracts showed antioxidant activity by inhibiting lipid peroxidation and oxidative hemolysis, with the seed extract exhibiting the strongest antifungal effect against Aspergillus versicolor, likely due to its high spermidine derivative content. These findings shed light on the potential of S. quitoense fruit for the development of functional foods, antioxidant-rich beverages, and nutraceutical products.

17 May 2023 in Engineering Proceedings
Verified
Conference Paper
Open access
PDF file
Alexis PereiraAlexis Pereira
Alexis Pereira
Mikel Añibarro-OrtegaMikel Añibarro-Ortega
Mikel Añibarro-Ortega
António NogueiraAntónio Nogueira
António Nogueira
Lillian BarrosLillian Barros
Lillian Barros
José PinelaJosé Pinela
José Pinela
The agri-food industry generates tons of waste rich in dietary fiber, a nutrient that can be recovered to be reused in the development of fiber-enriched foods and beverages. This could be a strategy to achieve resource-use efficiency and to promote adequate intakes of this nutrient, since a large part of the world population does not get the recommended daily amount. In this sense, this work was carried out to optimize the extraction of dietary fiber from quince (Cydonia oblonga Mill.) peel, using the response surface methodology. A 20-run experimental design was implemented, combining the factors time, temperature, and ethanol percentage at five levels. The yield of fibrous residue (FR) and its dietary fiber content and color parameters were used as dependent variables. The developed predictive models were statistically validated and used to determine optimal extraction conditions. The process was significantly affected by temperature and ethanol percentage, and the highest dietary fiber content (67% of FR) was obtained using 36% ethanol at 92 °C. Overall, these results showed that C. oblonga fruit peel could be upcycled into dietary fiber-rich food ingredients.