Aluminum (Al) toxicity is the main factor limiting plant growth and the yield of cereal crops in acidic soils. Al-induced oxidative stress could lead to the excessive accumulation of reactive oxygen species (ROS) and aldehydes in plants. Aldehyde dehydrogenase (ALDH) genes, which play an important role in detoxification of aldehydes when exposed to abiotic stress, have been identified in most species. However, little is known about the function of this gene family in the response to Al stress. Here, we identified an ALDH gene in maize, ZmALDH, involved in protection against Al-induced oxidative stress. Al stress up-regulated ZmALDH expression in both the roots and leaves. The expression of ZmALDH only responded to Al toxicity but not to other stresses including low pH and other metals. The heterologous overexpression of ZmALDH in Arabidopsis increased Al tolerance by promoting the ascorbate-glutathione cycle, increasing the transcript levels of antioxidant enzyme genes as well as the activities of their products, reducing MDA, and increasing free proline synthesis. The overexpression of ZmALDH also reduced Al accumulation in roots. Taken together, these findings suggest that ZmALDH participates in Al-induced oxidative stress and Al accumulation in roots, conferring Al tolerance in transgenic Arabidopsis.

Cadmium (Cd) can adversely damage plant growth. Therefore, understanding the control molecular mechanisms of Cd accumulation will benefit the development of strategies to reduce Cd accumulation in plants. This study performed transcriptomic and metabolomic analyses on the roots of a Cd-tolerant Tartary buckwheat cultivar following 0 h (CK), 6 h (T1), and 48 h (T2) of Cd treatment. The fresh weight and root length were not significantly inhibited under the T1 treatment but they were in the T2 treatment. The root’s ultrastructure was seriously damaged in T2 but not in T1 treatment. This was evidenced by deformed cell walls, altered shape and number of organelles. A total of 449, 999 differentially expressed genes (DEGs) and eight, 37 differentially expressed metabolites (DEMs) were identified in the CK versus T1 and CK versus T2 comparison, respectively. DEGs analysis found that the expression of genes related to cell wall function, glutathione (GSH) metabolism, and phenylpropanoid biosynthesis changed significantly during Cd stress. Several WRKY, MYB, ERF, and bHLH transcription factors and transporters also responded to Cd treatment. Our results indicate that Cd stress affects cell wall function and GSH metabolism and that changes in these pathways might contribute to mechanisms of Cd tolerance in Tartary buckwheat.

Cadmium (Cd) is a naturally occurring toxic heavy metal that adversely affects plant germination, growth, and development. While the effects of Cd have been described on many crop species including rice, maize, wheat and barley, few studies are available on cadmium’s effect on Tartary buckwheat which is a traditional grain in China. We examined nine genotypes and found that 30 µM of Cd reduced the root length in seedlings by between 4 and 44% and decreased the total biomass by 7 to 31%, compared with Cd-free controls. We identified a significant genotypic variation in sensitivity to Cd stress. Cd treatment decreased the total root length and the emergence and growth of lateral roots, and these changes were significantly greater in the Cd-sensitive genotypes than in tolerant genotypes. Cd resulted in greater wilting and discoloration in sensitive genotypes than in tolerant genotypes and caused more damage to the structure of root and leaf cells. Cd accumulated in the roots and shoots, but the concentrations in the sensitive genotypes were significantly greater than in the more tolerant genotypes. Cd treatment affected nutrient uptake, and the changes in the sensitive genotypes were greater than those in the tolerant genotypes, which could maintain their concentrations closer to the control levels. The induction of SOD, POD, and CAT activities in the roots and shoots was significantly greater in the tolerant genotypes than in the sensitive genotypes. We demonstrated that Cd stress reduced root and shoot growth, decreased plant biomass, disrupted nutrient uptake, altered cell structure, and managed Cd-induced oxidative stress differently in the sensitive and tolerant genotypes of Tartary buckwheat.

Tartary buckwheat (Fagopyrum tataricum Gaertn.) is a coarse grain crop rich in flavonoids that are beneficial to human health because they function as anti-inflammatories and provide protection against cardiovascular disease and diabetes. Flavonoid biosynthesis is a complex process, and relatively little is known about the regulatory pathways involved in Tartary buckwheat. Here, we cloned and characterized the FtMYB163 gene from Tartary buckwheat, which encodes a member of the R2R3-MYB transcription factor family. Amino acid sequence and phylogenetic analysis indicate that FtMYB163 is a member of subgroup 7 (SG7) and closely related to FeMYBF1, which regulates flavonol synthesis in common buckwheat (F. esculentum). We demonstrated that FtMYB163 localizes to the nucleus and has transcriptional activity. Expression levels of FtMYB163 in the roots, stems, leaves, flowers, and seeds of F. tataricum were positively correlated with the total flavonoid contents of these tissues. Overexpression of FtMYB163 in transgenic Arabidopsis enhanced the expression of several genes involved in early flavonoid biosynthesis (AtCHS, AtCHI, AtF3H, and AtFLS) and significantly increased the accumulation of several flavonoids, including naringenin chalcone, naringenin-7-O-glucoside, eriodictyol, and eight flavonol compounds. Our findings demonstrate that FtMYB163 positively regulates flavonol biosynthesis by changing the expression of several key genes in flavonoid biosynthetic pathways.

Background: Chalcone synthase (CHS) functions as a pivotal and initiating enzyme in the flavonoid biosynthesis pathway within plants, playing a crucial role in the accumulation and metabolic processes of flavonoids. Despite its importance, there has been no comprehensive analysis or detailed description of the CHS gene family members specifically in Tartary buckwheat. Methods: Based on a comprehensive analysis using multiple bioinformatics approaches and quantitative real−time PCR (qRT−PCR) technology, this study systematically identified and characterized the CHS gene family members from the complete genome sequence of Tartary buckwheat. Results: In this study, we identified a total of 14 FtCHS genes (FtCHS1−FtCHS14) in Tartary buckwheat. Analysis of gene structure and protein motifs showed that most FtCHS genes consist of two exons and a single intron, featuring conserved Chal−sti−synt_N and Chal−sti−synt_C domains. Phylogenetic studies suggested that FtCHS genes can be categorized into four primary groups: Groups I, II, III, and IV. Further analysis of the promoter regions revealed that the FtCHS family genes contain multiple cis−acting elements that respond to light, plant hormones, stress, and developmental cues. By combining phylogenetic analysis with gene expression data, we found that the genes in Group II (FtCHS3, FtCHS4, FtCHS5, and FtCHS6) exhibit significantly elevated expression levels specifically in flowers. Conclusions: Our study indicated that FtCHS is a gene superfamily comprising at least four functional members. The expression patterns of these FtCHS genes suggest their probable involvement in flower−related biological processes in Tartary buckwheat. This work provides fundamental insights into the comprehensive understanding of the functional roles of the CHS gene family in Tartary buckwheat.