In nanotechnology, the synthesis of carbon quantum dots (CQDs) by mixed doping with metals and non-metals has emerged as an appealing path of investigation. This review offers comprehensive insights into the synthesis, properties, and emerging applications of mixed-doped CQDs, underlining their potential for revolutionary advancements in chemical sensing, biosensing, bioimaging, and, thereby, contributing to advancements in diagnostics, therapeutics, and the under standing of complex biological processes. This synergistic combination enhances their sensitivity and selectivity towards specific chemical analytes. The resulting CQDs exhibit remarkable fluorescence properties that can be involved in precise chemical sensing applications. These metal-modified CQDs show their ability in the selective and sensitive detection from Hg to Fe and Mn ions. By influencing their exceptional fluorescence properties, they enable precise detection and monitoring of biomolecules, such as uric acid, cholesterol, and many antibiotics. Moreover, when it comes to bioimaging, these doped CQDs show unique behavior towards detecting cell lines. Their ability to emit light across a wide spectrum enables high-resolution imaging with minimal background noise. We uncover their potential in visualizing different cancer cell lines, offering valuable insights into cancer research and diagnostics. In conclusion, the synthesis of mixed-doped CQDs opens the way for revolutionary advancements in chemical sensing, biosensing, and bioimaging. As we investigate deeper into this field, we unlock new possibilities for diagnostics, therapeutics, and understanding complex biological processes.

Aims: Disseminated intravascular coagulation (DIC) is a common complication of heat stroke (HS) patients, and it is one of the important reasons leading to multiple organ failure and even death. The association between neutrophil extracellular traps (NETs) and DIC is unclear in HS mice. Methods and results: Here, HS was induced by the combination of hyperthermia (HT) and lipopolysaccharide (LPS). The DIC was evaluated by measuring prothrombin time (PT), D-dimer, thrombomodulin (TM), fibrinogen (FIB), and platelet (PLT). The expression of citrullinated-histone (CitH3) was analyzed by Western blotting. The formation of NETs was observed by immunofluorescence microscopy. The risk of HS-induced DIC was increased when HT was combined with LPS. The markers of NETs were significantly higher than those in the control group, and the NETs derived from HS promoted the development of DIC. DNase I improved coagulation dysfunction via the clearance of NETs caused by neutrophil aggregation. Conclusions: Degradation of NETs reduced the risk of developing DIC, and thus the survival rate of mice was improved. These results indicate that NETs may hold potential alternative therapeutic strategies for the treatment of DIC in HS patients.

The widespread presence of antibiotic residues, particularly norfloxacin (NFX), in food products and the environment has raised concern, underscoring the need for sensitive and selective detection methods. In this study, a novel broccoli-derived nitrogen-doped carbon quantum dots (N-CQDs) was synthesized via a green hydrothermal approach, 4-dimethylaminopyridine (DMAP) as both a nitrogen dopant and a functionalizing agent. The synthesized N-CQDs exhibit an average diameter of approximately ~4.2 nm and emit bright blue fluorescence, with a maximum emission at 445 nm upon excitation at 360 nm. A “Turn-ON” response toward NFX was achieved with a detection limit of 0.30 nM, attributed to hydrogen bonding and π–π stacking interactions that suppressed non-radiative decay. Moreover, the sensor demonstrates high selectivity for NFX, effectively distinguishing it from common interfering substances, including other antibiotics, organic acids, and biomolecules. The N-CQDs also exhibit excellent stability under diverse conditions, such as varying pH levels, high ionic strength, and prolonged irradiation. Finally, the practical applicability of the developed sensor was validated by detecting NFX in spiked broccoli extract and milk samples, with recovery rates ranging from 98.2% to 100.1% and relative standard deviations of less than 2.0%. This work presents a sustainable and efficient N-CQD-based fluorescent sensing platform, offering significant potential for rapid and reliable detection of NFX in food safety and environmental monitoring.

Euphorbia uralensis belongs to the family Euphorbiaceae and is widely distributed in northern Xinjiang, making it a characteristic plant of the region in Xinjiang, China. The chemical composition and biological activity of Euphorbia uralensis have not yet been reported, although certain compounds isolated from Euphorbia plants in Xinjiang, China, have demonstrated exceptional multidrug resistance (MDR) reversal. This study aims to investigate the chemical components present in Euphorbia uralensis with the potential to reverse MDR. The aerial parts of Euphorbia uralensis were extracted using organic solvents of varying polarities, resulting in dichloromethane (Fr-E) and petroleum ether (Fr-S) fractions, which exhibited greater MDR reversal activity than the other fractions. The chemical constituents of the Fr-S fraction were analyzed using GC-MS. The chemical components of the Fr-E fraction were isolated and purified using column chromatography. The most effective compounds with MDR reversal activity were screened out, and the mechanism was investigated using molecular docking, molecular dynamics simulations, Western blotting, and rhodamine 123 staining. GC-MS analysis showed that the Fr-S fraction was rich in triterpenes, fatty acids, phenols, and long-chain alkanes, all of which were identified for the first time in Euphorbia uralensis. Among these, palmitic acid was present at a content level of 15.86%. This study notably unveils the discovery of a new compound and 16 previously recorded compounds for the first time in this plant, with the main types identified as steroids, sesquiterpenes, and flavonoids. The isolated compounds were tested for cytotoxicity and MDR reversal activity. The new compounds Euphouralosides A, pubinernoid A, naringenin, and punigratine showed good MDR reversal activity against MCF-7 and MCF-7/ADR cell lines. Punigratine was the most active compound. Moreover, punigratine could stably bind to the ABCB1 protein. Western blot analysis revealed that punigratine did not affect the expression of the ABCB1 protein in cells (p > 0.05). However, following treatment with punigratine (0.16 μM), there was a significant increase the intracellular accumulation of Rh123 in MCF-7/ADR cells (p < 0.05). These findings suggest that punigratine can inhibit the efflux of the ABCB1 protein, thereby overcoming MDR in tumors. This study provides a foundation for further research on the biological activity and medicinal potential of Euphorbia uralensis.

Glioblastoma multiforme (GBM) is an incurable primary brain malignancy characterized by prominent chemoresistance and poor prognosis. Herein, we report an in vitro proof-of-concept study of a green, ligand-functionalized carbon quantum dot (CQD) for combined drug delivery with pH-responsive release in glioblastoma cells. Nitrogen-doped red-emissive N-CQDs were synthesized via a hydrothermal method using locust biomass as a renewable carbon precursor, functionalized with glucuronic acid for active tumor targeting, and loaded with piperlongumine (PL) and silibinin (SIL) drugs at an optimized mass ratio. The nanosystem was characterized by XRD, FTIR, SEM, TEM, XPS, and optical spectroscopy, and evaluated in vitro for drug release, cellular uptake, apoptosis, ROS levels, and caspase-3 activity. The optimized nanoplatform exhibited uniform morphology (average size 2.76 nm), strong red fluorescence (Ex490nm/Em620nm), high dual-drug loading efficiency (46.0%), and pH-responsive release behavior with rapid drug liberation in the acidic tumor microenvironment (pH 5.5) and minimal leakage at physiological pH (pH 7.4). The nanosystem demonstrated efficient cellular internalization, dual mitochondrial/nuclear targeting, and enhanced combined antitumor effect via ROS overproduction and activation of the caspase-3 pathway. This work establishes a green and multifunctional nanotherapeutic platform with promising potential for future glioblastoma-directed drug delivery applications.