Post-cholecystectomy syndrome (PCS) is a medical condition that can develop after cholecystectomy. It is characterized by several gastrointestinal symptoms, including abdominal pain, dyspepsia, bloating, and diarrhea, which can limit patients' quality of life. Understanding the underlying cause, risk factors, and effective intervention is essential to improve patient safety in clinical practice. This study aims to evaluate the frequency of post-cholecystectomy syndrome (PCS), analyze its symptoms and demographic features, and identify likely risk factors. This observational longitudinal study included 200 patients who underwent laparoscopic cholecystectomy in the surgical department of Shaikh Zayed Hospital Lahore between Jan 1st, 2021, and Jan 1st, 2022. Data were retrieved from electronic medical records, and patients were followed for PCS. The data was analyzed using SPSS software, version 25. Of 200 patients, 45 (22.5%) were diagnosed with Pcs. The ratio of males to females was 1:1.64. Age ranges from 19-78, and most belong to the 5th decade of life. Common symptoms are right upper quadrant abdominal pain in 33 (73.3%), nausea in 23 (51.1%), dyspepsia, vomiting in 16 (35.6%), and presenting signs included right hypochondriac region tenderness in 18 (40%), jaundice and epigastric tenderness in 4 (8.9%) cases. Common causes included Psychiatric disease in 8 (17.8%), helicobacter pylori infection in 7 (15.6%), muscle pathology in 6 (13.3%), and gastritis in 5 (11.11%), respectively. Based on the results it can be concluded that to provide effective treatments for individuals with PCS, it is essential to take signs seriously, conduct a comprehensive evaluation to reach an accurate diagnosis and treat the underlying cause appropriately.