The polymerase chain reaction (PCR) technology is a mainstream detection method used in medical diagnoses, environmental monitoring, food hygiene, and safety. However, the systematic analysis of a compact structure with fast temperature changes for an ultra-fast PCR device that is convenient for on-site detection still lacks investigation. To overcome the problems of low heating efficiency and non-portability of PCR devices currently used, a miniaturized PCR system based on a microfluidic chip, i.e., lab-on-chip technology, has been proposed. The main objective of this paper is to explore the feasibility of using a heat resistor that can reach a fast heating rate and temperature uniformity combined with air cooling technology for rapid cooling and to investigate the influences of various pattern designs and thicknesses of the resistor on heating rates and temperature uniformity. Additionally, a PCR chip made of various materials with different thermal properties, such as surface emissivity, thermal conductivity, mass density, and heat capacity at constant pressure is analyzed. In addition to the heat loss caused by the natural convection of air, the radiation loss of the simulation object is also considered, which makes the model much closer to the practical situation. Our research results provide a considerable reference for the design of the heating and cooling modules used in the ultra-fast PCR protocol, which has great potential in In Vitro Diagnosis (IVD) and the PCR detection of foodborne pathogens and bacteria.

The color reflector with multiple-layer thin film scheme has attracted much attention because of the potential for massive production by wafer-scale deposition and the possibility to integrate with photonics (semiconductor) devices. Here, an angle-insensitive green reflector with a simple multilayer dielectric thin film structure was reported, with predicted chromatic coordinates based on CIE 1931 standard. The SiN/SiO₂ multilayer thin film stack, including a special silicon-rich nitride material with ultrahigh refractive index, was grown alternatively by an inductively coupled plasma chemical vapor deposition (ICPCVD) system at a low stage temperature of 80 °C. The green reflector showed a maximum reflectivity of 73% around 561 nm with a full width at half maximum (FWHM) of 87 nm in the visible wavelength range, which contributed significantly to its color appearance. The measurement by an angle-resolved spectrometer under the illumination of p/s-polarized light wave with a variable angle of incidence indicated that the reflectance spectrum blue-shifted slightly with the increasing of incident angle such that the green color could be kept.

Industrial-grade optical semiconductor films have attracted considerable research interest because of their potential for wafer-scale mass deposition and direct integration with other optoelectronic wafers. The development of optical thin-film processes that are compatible with complementary metal-oxide-semiconductor (CMOS) processes will be beneficial for the improvement of chip integration. In this study, a multilayer periodically structured optical film containing Fabry–Perot cavity was designed, utilizing nine pairs of SiN/SiO₂ dielectrics. Subsequently, the multilayer films were deposited on Si substrates through the inductively coupled plasma chemical vapor deposition (ICPCVD) technique, maintaining a low temperature of 80 °C. The prepared films exhibit narrow bandpass characteristics with a maximum peak transmittance of 76% at 690 nm. Scanning electron microscopy (SEM) shows that the film structure has good periodicity. In addition, when the optical films are exposed to p/s polarized light at different angles of incidence, the cavity mode of the film undergoes a blueshift, which greatly affects the color appearance of the film. As the temperature rises, the cavity mode undergoes a gradual redshift, while the full width at half maximum (FWHM) and quality factor remain relatively constant.

The increasing spread of infectious diseases caused by pathogenic microorganisms underscores the urgent need for highly sensitive, portable, and rapid nucleic acid detection technologies to facilitate early diagnosis and effective prevention. In this study, we developed a fluorescence-based nucleic acid detection platform that integrates a microfluidic chip with an all-inorganic perovskite photodetector. The system enables integrated operation of nucleic acid extraction, purification, and amplification on a microfluidic chip, combined with real-time electrical signal readout via a CsPbBr3 perovskite photodetector. Experimental results indicate that the photodetector exhibits high responsivity at 530 nm, aligning well with the primary emission peak of FAM. The system demonstrates a strong linear correlation between photocurrent and FAM concentration over the range of 0.01–0.4 μM (R2 = 0.928), with a low detection limit of 0.01 μM and excellent reproducibility across multiple measurements. Validation using FAM standard solutions and Escherichia coli samples confirmed the system’s reliable linearity and signal stability. This platform demonstrates strong potential for rapid pathogen screening and point-of-care diagnostic applications.