Photobiomodulation, showing positive effects on wound healing processes, has been performed mainly with lasers in the red/infrared spectrum. Light of shorter wavelengths can significantly influence biological systems. This study aimed to evaluate and compare the therapeutic effects of pulsed LED light of different wavelengths on wound healing in a diabetic (db/db) mouse excision wound model. LED therapy by Repuls was applied at either 470 nm (blue), 540 nm (green) or 635 nm (red), at 40 mW/cm² each. Wound size and wound perfusion were assessed and correlated to wound temperature and light absorption in the tissue. Red and trend-wise green light positively stimulated wound healing, while blue light was ineffective. Light absorption was wavelength-dependent and was associated with significantly increased wound perfusion as measured by laser Doppler imaging. Shorter wavelengths ranging from green to blue significantly increased wound surface temperature, while red light, which penetrates deeper into tissue, led to a significant increase in core body temperature. In summary, wound treatment with pulsed red or green light resulted in improved wound healing in diabetic mice. Since impeded wound healing in diabetic patients poses an ever-increasing socio-economic problem, LED therapy may be an effective, easily applied and cost-efficient supportive treatment for diabetic wound therapy.

Objective: This randomized, double-blind, placebo-controlled clinical trial investigated the therapeutic efficacy of pulsed electromagnetic field (PEMF) therapy in dogs diagnosed with chronic osteoarthritis (OA). Methods: Twenty-one dogs with radiographically confirmed OA in at least one limb were randomized into treatment (n = 10) and placebo (n = 11) groups. PEMF therapy was administered over six weeks. Gait symmetry indices for peak vertical force and vertical impulse were assessed using kinetic analysis at baseline (Day 0), mid-treatment (Day 21), and post-treatment (Day 42). Lameness and pain scores, additional treatments, and dropout rates were recorded. Owners completed the Liverpool Osteoarthritis in Dogs (LOAD) questionnaire at each time point. An Overall Treatment Effectiveness (OTE) score was computed based on objective and subjective outcomes. Results: PEMF-treated dogs exhibited significant improvements in gait symmetry by Day 42 (p = 0.030). LOAD scores declined steadily in the treatment group, although no statistically significant differences were observed between groups. This trend may suggest a potential reduction in pain and improvement in mobility. No significant between-group differences were found for the OTE score. Conclusion: PEMF therapy appears to be a safe, non-invasive, and potentially effective adjunctive or stand-alone modality for the management of pain and mobility impairment associated with osteoarthritis in dogs. Further research is warranted to confirm long-term efficacy and optimize treatment protocols.