The control of antimicrobial resistance (AMR) seems to have come to a dead end. The major consequences of the use and abuse of antibacterial drugs are the development of resistant strains due to genetic mutability of both pathogenic and nonpathogenic microorganisms. We, herein, report the synthesis, characterization and biological activities of coumarin-thiazole-pyrazole (CTP) molecular hybrids with an effort to explore and overcome the increasing antimicrobial resistance. The compounds were characterized by analyzing their IR, Mass, ¹H and¹³C NMR spectral data and elemental analysis. The in vitro antimicrobial activity of the synthesized compounds was investigated against various pathogenic strains; the results obtained were further explained with the help of DFT and molecular orbital calculations. Compound 1b and 1f displayed good antimicrobial activity and synergistic effects when used with kanamycin and amphotericin B. Furthermore, in vitro cytotoxicity of compounds 1b and 1f were studied against HeLa cells (cervical cancer cell) and Hek-293 cells. The results of molecular docking study were used to better rationalize the action and prediction of the binding modes of these compounds.

A luminescent 3D metal–organic framework [Zn(NDA)(AMP)] = PUC1 (where, NDA = naphthalene-2,6-dicarboxylic acid and AMP = 4-aminomethyl pyridine) was synthesized under solvothermal conditions. The synthesized 3D framework was fully characterized with the help of different analytical techniques such as SCXRD, FTIR, TGA, PXRD, SEM, BET, etc. PUC1 exhibited a strong emission peak at 371 nm when excited at 290 nm and the resulting emission was efficiently quenched in the presence of various organic explosive substances like pentaerythritol tetranitrate (PETN), 2,4,6-trinitrophenyl-N-methylnitramine (Tetryl), trinitrotoluene (TNT), 1,3,5-trinitroperhydro-1,3,5-triazine (RDX), and 1,3,5,7-tetranitro-1,3,5,7-tetrazoctane (HMX). PUC1 revealed highly sensitive and selective detection of PETN and Tetryl with high quenching constant values of 0.1 × 10⁶ and 0.12 × 10⁵ M⁻¹ and low detection limits of 0.315 and 0.404 μM respectively. The strong luminescent properties of PUC1 lead to its successful application in the development of latent fingermarks on different non-porous surfaces using the powder dusting method. The accuracy and applicability of the synthesized material were determined by developing fingerprints by using secretions from eccrine and apocrine glands on a glass slide and various other surfaces, followed by dusting the surfaces. The results so obtained were found to be very accurate and promising.